Restriction map for
pFN21AE4534
(Linear) (Six-base) MAPSORT of: KIEE4534.seq Check: 5734 from: 1 to: 1797
KIEE4534.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AccI GT'mk_AC
Cuts at: 0 719 947 1797
Size: 719 228 850
Fragments arranged by size:
850 719 228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1708 1797
Size: 1708 89
AfeI AGC'GCT
Cuts at: 0 561 1797
Size: 561 1236
AflIII A'CryG_T
Cuts at: 0 134 1661 1797
Size: 134 1527 136
Fragments arranged by size:
1527 136 134
AleI CACnn'nnGTG
Cuts at: 0 477 1797
Size: 477 1320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1393 1797
Size: 1393 404
AlwNI CAG_nnn'CTG
Cuts at: 0 1475 1797
Size: 1475 322
ApaLI G'TGCA_C
Cuts at: 0 984 1797
Size: 984 813
AvaI C'yCGr_G
Cuts at: 0 784 916 1081 1645 1797
Size: 784 132 165 564 152
Fragments arranged by size:
784 564 165 152 132
BamHI G'GATC_C
Cuts at: 0 665 1465 1797
Size: 665 800 332
Fragments arranged by size:
800 665 332
BanII G_rGCy'C
Cuts at: 0 1265 1684 1797
Size: 1265 419 113
Fragments arranged by size:
1265 419 113
BbsI GAAGACnn'nnnn_
Cuts at: 0 1329 1619 1730 1797
Size: 1329 290 111 67
Fragments arranged by size:
1329 290 111 67
BclI T'GATC_A
Cuts at: 0 752 1797
Size: 752 1045
BfrBI ATG'CAT
Cuts at: 0 591 1797
Size: 591 1206
BglI GCCn_nnn'nGGC
Cuts at: 0 64 1797
Size: 64 1733
BglII A'GATC_T
Cuts at: 0 35 872 1797
Size: 35 837 925
Fragments arranged by size:
925 837 35
Bme1580I G_kGCm'C
Cuts at: 0 988 1797
Size: 988 809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 319 1797
Size: 222 97 1478
Fragments arranged by size:
1478 222 97
Bpu10I CC'TnA_GC
Cuts at: 0 1751 1797
Size: 1751 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 1309 1797
Size: 1309 488
BsaAI yAC'GTr
Cuts at: 0 137 1797
Size: 137 1660
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 940 970 1797
Size: 940 30 827
Fragments arranged by size:
940 827 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1234 1797
Size: 1234 563
BseYI C'CCAG_C
Cuts at: 0 891 1118 1173 1717 1797
Size: 891 227 55 544 80
Fragments arranged by size:
891 544 227 80 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1179 1570 1797
Size: 121 1058 391 227
Fragments arranged by size:
1058 391 227 121
BsiEI CG_ry'CG
Cuts at: 0 129 1797
Size: 129 1668
BsiHKAI G_wGCw'C
Cuts at: 0 307 988 1265 1797
Size: 307 681 277 532
Fragments arranged by size:
681 532 307 277
Bsp1286I G_dGCh'C
Cuts at: 0 307 988 1265 1684 1797
Size: 307 681 277 419 113
Fragments arranged by size:
681 419 307 277 113
BsrDI GCAATG_nn'
Cuts at: 0 1055 1268 1797
Size: 1055 213 529
Fragments arranged by size:
1055 529 213
BsrGI T'GTAC_A
Cuts at: 0 452 1797
Size: 452 1345
BssSI C'ACGA_G
Cuts at: 0 1387 1797
Size: 1387 410
BstEII G'GTnAC_C
Cuts at: 0 1113 1797
Size: 1113 684
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 189 564 651 1797
Size: 87 102 375 87 1146
Fragments arranged by size:
1146 375 102 87 87
BstYI r'GATC_y
Cuts at: 0 35 626 665 872 1465 1797
Size: 35 591 39 207 593 332
Fragments arranged by size:
593 591 332 207 39 35
Bsu36I CC'TnA_GG
Cuts at: 0 1074 1797
Size: 1074 723
BtgI C'CryG_G
Cuts at: 0 478 768 1568 1631 1797
Size: 478 290 800 63 166
Fragments arranged by size:
800 478 290 166 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 642 1797
Size: 642 1155
BtsI GCAGTG_nn'
Cuts at: 0 472 1797
Size: 472 1325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1502 1537 1797
Size: 1502 35 260
Fragments arranged by size:
1502 260 35
EaeI y'GGCC_r
Cuts at: 0 23 65 634 641 653 1797
Size: 23 42 569 7 12 1144
Fragments arranged by size:
1144 569 42 23 12 7
EarI CTCTTCn'nnn_
Cuts at: 0 295 811 1042 1156 1270 1797
Size: 295 516 231 114 114 527
Fragments arranged by size:
527 516 295 231 114 114
EcoICRI GAG'CTC
Cuts at: 0 1263 1797
Size: 1263 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 319 1708 1797
Size: 222 97 1389 89
Fragments arranged by size:
1389 222 97 89
EcoO109I rG'GnC_Cy
Cuts at: 0 57 282 902 1078 1472 1797
Size: 57 225 620 176 394 325
Fragments arranged by size:
620 394 325 225 176 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 165 1797
Size: 165 1632
HaeII r_GCGC'y
Cuts at: 0 563 1797
Size: 563 1234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 995 1027 1342 1374 1797
Size: 995 32 315 32 423
Fragments arranged by size:
995 423 315 32 32
HincII GTy'rAC
Cuts at: 0 540 1431 1797
Size: 540 891 366
Fragments arranged by size:
891 540 366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1443 1797
Size: 548 895 354
Fragments arranged by size:
895 548 354
MscI TGG'CCA
Cuts at: 0 67 636 643 655 1797
Size: 67 569 7 12 1142
Fragments arranged by size:
1142 569 67 12 7
MslI CAynn'nnrTG
Cuts at: 0 477 941 991 1797
Size: 477 464 50 806
Fragments arranged by size:
806 477 464 50
MspA1I CmG'CkG
Cuts at: 0 515 632 1122 1797
Size: 515 117 490 675
Fragments arranged by size:
675 515 490 117
NcoI C'CATG_G
Cuts at: 0 1568 1631 1797
Size: 1568 63 166
Fragments arranged by size:
1568 166 63
NsiI A_TGCA'T
Cuts at: 0 593 1797
Size: 593 1204
NspI r_CATG'y
Cuts at: 0 591 996 1665 1797
Size: 591 405 669 132
Fragments arranged by size:
669 591 405 132
PasI CC'CwG_GG
Cuts at: 0 309 1797
Size: 309 1488
PciI A'CATG_T
Cuts at: 0 1661 1797
Size: 1661 136
PflMI CCAn_nnn'nTGG
Cuts at: 0 662 1458 1605 1707 1744 1797
Size: 662 796 147 102 37 53
Fragments arranged by size:
796 662 147 102 53 37
PmlI CAC'GTG
Cuts at: 0 137 1797
Size: 137 1660
PpuMI rG'GwC_Cy
Cuts at: 0 282 902 1797
Size: 282 620 895
Fragments arranged by size:
895 620 282
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 400 432 1797
Size: 400 32 1365
Fragments arranged by size:
1365 400 32
PvuII CAG'CTG
Cuts at: 0 1122 1797
Size: 1122 675
SacI G_AGCT'C
Cuts at: 0 1265 1797
Size: 1265 532
SanDI GG'GwC_CC
Cuts at: 0 282 902 1797
Size: 282 620 895
Fragments arranged by size:
895 620 282
SapI GCTCTTCn'nnn_
Cuts at: 0 1270 1797
Size: 1270 527
SfcI C'TryA_G
Cuts at: 0 528 948 1797
Size: 528 420 849
Fragments arranged by size:
849 528 420
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 64 1797
Size: 64 1733
SmaI CCC'GGG
Cuts at: 0 786 1083 1647 1797
Size: 786 297 564 150
Fragments arranged by size:
786 564 297 150
StuI AGG'CCT
Cuts at: 0 1789 1797
Size: 1789 8
StyI C'CwwG_G
Cuts at: 0 86 791 1568 1631 1797
Size: 86 705 777 63 166
Fragments arranged by size:
777 705 166 86 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 834 1291 1797
Size: 834 457 506
Fragments arranged by size:
834 506 457
TatI w'GTAC_w
Cuts at: 0 317 452 1797
Size: 317 135 1345
Fragments arranged by size:
1345 317 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 809 841 1797
Size: 216 32 561 32 956
Fragments arranged by size:
956 561 216 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 87 531 564 1062 1459 1797
Size: 87 444 33 498 397 338
Fragments arranged by size:
498 444 397 338 87 33
XmaI C'CCGG_G
Cuts at: 0 784 1081 1645 1797
Size: 784 297 564 152
Fragments arranged by size:
784 564 297 152
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI
AvaI BamHI BanII BbsI BclI BfrBI BglI BglII
Bme1580I BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BsrDI BsrGI BssSI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NcoI NsiI NspI PasI
PciI PflMI PmlI PpuMI PsrI PvuII SacI SanDI
SapI SfcI SfiI SmaI StuI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbvCI BcgI BciVI BlpI BmgBI BmrI BmtI
BplI BpuEI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BssHII BstAPI BstBI
BstZ17I ClaI DraI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI
ZraI