Restriction map for
pFN21AE4532
(Linear) (Six-base) MAPSORT of: KIEE4532.seq Check: 2480 from: 1 to: 1782
KIEE4532.orf
With 182 enzymes: SgfI *
March 22, 2012 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1049 1782
Size: 1049 733
AccI GT'mk_AC
Cuts at: 0 788 1085 1782
Size: 788 297 697
Fragments arranged by size:
788 697 297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1012 1512 1765 1782
Size: 568 444 500 253 17
Fragments arranged by size:
568 500 444 253 17
AflIII A'CryG_T
Cuts at: 0 306 1782
Size: 306 1476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 133 1782
Size: 133 1649
AlwNI CAG_nnn'CTG
Cuts at: 0 690 990 1113 1132 1731 1782
Size: 690 300 123 19 599 51
Fragments arranged by size:
690 599 300 123 51 19
ApaI G_GGCC'C
Cuts at: 0 667 1782
Size: 667 1115
AvaI C'yCGr_G
Cuts at: 0 362 1782
Size: 362 1420
BamHI G'GATC_C
Cuts at: 0 1702 1782
Size: 1702 80
BanI G'GyrC_C
Cuts at: 0 1078 1782
Size: 1078 704
BanII G_rGCy'C
Cuts at: 0 667 1022 1782
Size: 667 355 760
Fragments arranged by size:
760 667 355
BbsI GAAGACnn'nnnn_
Cuts at: 0 303 967 1782
Size: 303 664 815
Fragments arranged by size:
815 664 303
BbvCI CC'TCA_GC
Cuts at: 0 1052 1133 1782
Size: 1052 81 649
Fragments arranged by size:
1052 649 81
BfrBI ATG'CAT
Cuts at: 0 45 1782
Size: 45 1737
BglI GCCn_nnn'nGGC
Cuts at: 0 601 1782
Size: 601 1181
BlpI GC'TnA_GC
Cuts at: 0 605 1021 1782
Size: 605 416 761
Fragments arranged by size:
761 605 416
Bme1580I G_kGCm'C
Cuts at: 0 667 1126 1346 1562 1782
Size: 667 459 220 216 220
Fragments arranged by size:
667 459 220 220 216
BmgBI CAC'GTC
Cuts at: 0 309 1782
Size: 309 1473
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 217 1782
Size: 217 1565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 526 974 1782
Size: 232 294 448 808
Fragments arranged by size:
808 448 294 232
Bpu10I CC'TnA_GC
Cuts at: 0 1052 1133 1782
Size: 1052 81 649
Fragments arranged by size:
1052 649 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 793 920 1782
Size: 793 127 862
Fragments arranged by size:
862 793 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 703 733 1782
Size: 205 30 468 30 1049
Fragments arranged by size:
1049 468 205 30 30
BseYI C'CCAG_C
Cuts at: 0 205 683 715 1782
Size: 205 478 32 1067
Fragments arranged by size:
1067 478 205 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1027 1782
Size: 287 740 755
Fragments arranged by size:
755 740 287
BsiHKAI G_wGCw'C
Cuts at: 0 731 839 1022 1782
Size: 731 108 183 760
Fragments arranged by size:
760 731 183 108
Bsp1286I G_dGCh'C
Cuts at: 0 667 731 839 1022 1126 1346 1562 1782
Size: 667 64 108 183 104 220 216 220
Fragments arranged by size:
667 220 220 216 183 108 104 64
BspHI T'CATG_A
Cuts at: 0 149 1782
Size: 149 1633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1049 1782
Size: 1049 733
BsrBI CCG'CTC
Cuts at: 0 201 1389 1782
Size: 201 1188 393
Fragments arranged by size:
1188 393 201
BstEII G'GTnAC_C
Cuts at: 0 1665 1782
Size: 1665 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1182 1782
Size: 1182 600
BstYI r'GATC_y
Cuts at: 0 1702 1782
Size: 1702 80
BstZ17I GTA'TAC
Cuts at: 0 789 1782
Size: 789 993
BtgI C'CryG_G
Cuts at: 0 1118 1782
Size: 1118 664
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 870 1782
Size: 870 912
BtsI GCAGTG_nn'
Cuts at: 0 868 1602 1782
Size: 868 734 180
Fragments arranged by size:
868 734 180
EaeI y'GGCC_r
Cuts at: 0 1115 1782
Size: 1115 667
EcoICRI GAG'CTC
Cuts at: 0 1020 1782
Size: 1020 762
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 526 568 974 1012 1512 1765 1782
Size: 232 294 42 406 38 500 253 17
Fragments arranged by size:
500 406 294 253 232 42 38 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 844 1051 1782
Size: 844 207 731
Fragments arranged by size:
844 731 207
EcoO109I rG'GnC_Cy
Cuts at: 0 276 663 975 1110 1152 1578 1782
Size: 276 387 312 135 42 426 204
Fragments arranged by size:
426 387 312 276 204 135 42
EcoRV GAT'ATC
Cuts at: 0 180 1782
Size: 180 1602
HaeII r_GCGC'y
Cuts at: 0 513 1550 1782
Size: 513 1037 232
Fragments arranged by size:
1037 513 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 480 512 684 703 716 735 747 779
Size: 480 32 172 19 13 19 12 32
Cuts at: 779 1130 1154 1162 1186 1782
Size: 351 24 8 24 596
Fragments arranged by size:
596 480 351 172 32 32 24 24
19 19 13 12 8
HindIII A'AGCT_T
Cuts at: 0 462 1782
Size: 462 1320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 673 1782
Size: 9 664 1109
Fragments arranged by size:
1109 664 9
MscI TGG'CCA
Cuts at: 0 1117 1782
Size: 1117 665
MslI CAynn'nnrTG
Cuts at: 0 115 1324 1782
Size: 115 1209 458
Fragments arranged by size:
1209 458 115
MspA1I CmG'CkG
Cuts at: 0 806 1782
Size: 806 976
NcoI C'CATG_G
Cuts at: 0 1118 1782
Size: 1118 664
NruI TCG'CGA
Cuts at: 0 529 1782
Size: 529 1253
NsiI A_TGCA'T
Cuts at: 0 47 1782
Size: 47 1735
PfoI T'CCnGG_A
Cuts at: 0 614 695 839 1782
Size: 614 81 144 943
Fragments arranged by size:
943 614 144 81
PpuMI rG'GwC_Cy
Cuts at: 0 276 975 1110 1152 1782
Size: 276 699 135 42 630
Fragments arranged by size:
699 630 276 135 42
PshAI GACnn'nnGTC
Cuts at: 0 326 1782
Size: 326 1456
PspOMI G'GGCC_C
Cuts at: 0 663 1782
Size: 663 1119
SacI G_AGCT'C
Cuts at: 0 1022 1782
Size: 1022 760
ScaI AGT'ACT
Cuts at: 0 1030 1782
Size: 1030 752
SfcI C'TryA_G
Cuts at: 0 468 1782
Size: 468 1314
SspI AAT'ATT
Cuts at: 0 402 1782
Size: 402 1380
StyI C'CwwG_G
Cuts at: 0 437 1074 1118 1623 1782
Size: 437 637 44 505 159
Fragments arranged by size:
637 505 437 159 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 169 1051 1782
Size: 169 882 731
Fragments arranged by size:
882 731 169
TatI w'GTAC_w
Cuts at: 0 1028 1782
Size: 1028 754
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbsI BbvCI BfrBI BglI BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI EaeI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV HaeII Hin4I HindIII MmeI MscI
MslI MspA1I NcoI NruI NsiI PfoI PpuMI PshAI
PspOMI SacI ScaI SfcI SspI StyI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BclI BglII BmrI BmtI BpuEI
BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoRI FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI