Restriction map for
pFN21AE4529
(Linear) (Six-base) MAPSORT of: KIEE4529.seq Check: 6014 from: 1 to: 1740
KIEE4529.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 931 1740
Size: 931 809
AatII G_ACGT'C
Cuts at: 0 1154 1740
Size: 1154 586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 750 1740
Size: 730 20 990
Fragments arranged by size:
990 730 20
AflIII A'CryG_T
Cuts at: 0 422 1415 1740
Size: 422 993 325
Fragments arranged by size:
993 422 325
AleI CACnn'nnGTG
Cuts at: 0 860 1740
Size: 860 880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 214 1740
Size: 214 1526
AlwNI CAG_nnn'CTG
Cuts at: 0 408 1740
Size: 408 1332
ApaI G_GGCC'C
Cuts at: 0 1346 1740
Size: 1346 394
ApoI r'AATT_y
Cuts at: 0 670 1045 1740
Size: 670 375 695
Fragments arranged by size:
695 670 375
AvaI C'yCGr_G
Cuts at: 0 58 592 1098 1740
Size: 58 534 506 642
Fragments arranged by size:
642 534 506 58
BamHI G'GATC_C
Cuts at: 0 585 809 1740
Size: 585 224 931
Fragments arranged by size:
931 585 224
BanI G'GyrC_C
Cuts at: 0 1102 1171 1555 1740
Size: 1102 69 384 185
Fragments arranged by size:
1102 384 185 69
BanII G_rGCy'C
Cuts at: 0 254 1346 1740
Size: 254 1092 394
Fragments arranged by size:
1092 394 254
BbeI G_GCGC'C
Cuts at: 0 1559 1740
Size: 1559 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 122 718 858 1740
Size: 122 596 140 882
Fragments arranged by size:
882 596 140 122
BclI T'GATC_A
Cuts at: 0 776 1740
Size: 776 964
BglI GCCn_nnn'nGGC
Cuts at: 0 1441 1740
Size: 1441 299
BlpI GC'TnA_GC
Cuts at: 0 1528 1740
Size: 1528 212
Bme1580I G_kGCm'C
Cuts at: 0 1105 1174 1346 1740
Size: 1105 69 172 394
Fragments arranged by size:
1105 394 172 69
BmgBI CAC'GTC
Cuts at: 0 423 906 1740
Size: 423 483 834
Fragments arranged by size:
834 483 423
BmrI ACTGGGnnnn_n'
Cuts at: 0 439 854 1740
Size: 439 415 886
Fragments arranged by size:
886 439 415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 630 709 844 1740
Size: 630 79 135 896
Fragments arranged by size:
896 630 135 79
Bpu10I CC'TnA_GC
Cuts at: 0 480 1740
Size: 480 1260
BsaI GGTCTCn'nnnn_
Cuts at: 0 25 820 1740
Size: 25 795 920
Fragments arranged by size:
920 795 25
BsaAI yAC'GTr
Cuts at: 0 522 1740
Size: 522 1218
BsaBI GATnn'nnATC
Cuts at: 0 814 1120 1740
Size: 814 306 620
Fragments arranged by size:
814 620 306
BsaHI Gr'CG_yC
Cuts at: 0 1151 1556 1740
Size: 1151 405 184
Fragments arranged by size:
1151 405 184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 1480 1510 1740
Size: 358 30 1092 30 230
Fragments arranged by size:
1092 358 230 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 931 1612 1732 1740
Size: 931 681 120 8
Fragments arranged by size:
931 681 120 8
BseYI C'CCAG_C
Cuts at: 0 318 613 1267 1338 1481 1740
Size: 318 295 654 71 143 259
Fragments arranged by size:
654 318 295 259 143 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1740
Size: 253 1487
BsiHKAI G_wGCw'C
Cuts at: 0 1091 1529 1740
Size: 1091 438 211
Fragments arranged by size:
1091 438 211
BsmI GAATG_Cn'
Cuts at: 0 1722 1740
Size: 1722 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 136 1740
Size: 136 1604
Bsp1286I G_dGCh'C
Cuts at: 0 254 1091 1105 1174 1346 1529 1740
Size: 254 837 14 69 172 183 211
Fragments arranged by size:
837 254 211 183 172 69 14
BspHI T'CATG_A
Cuts at: 0 146 1109 1740
Size: 146 963 631
Fragments arranged by size:
963 631 146
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 931 1740
Size: 931 809
BsrBI CCG'CTC
Cuts at: 0 39 337 597 953 1038 1364 1670 1740
Size: 39 298 260 356 85 326 306 70
Fragments arranged by size:
356 326 306 298 260 85 70 39
BsrFI r'CCGG_y
Cuts at: 0 87 434 1552 1740
Size: 87 347 1118 188
Fragments arranged by size:
1118 347 188 87
BstAPI GCAn_nnn'nTGC
Cuts at: 0 188 1728 1740
Size: 188 1540 12
Fragments arranged by size:
1540 188 12
BstEII G'GTnAC_C
Cuts at: 0 460 1740
Size: 460 1280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 822 1137 1740
Size: 822 315 603
Fragments arranged by size:
822 603 315
BstYI r'GATC_y
Cuts at: 0 215 585 746 809 1740
Size: 215 370 161 63 931
Fragments arranged by size:
931 370 215 161 63
BtgI C'CryG_G
Cuts at: 0 450 1373 1662 1740
Size: 450 923 289 78
Fragments arranged by size:
923 450 289 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 279 1097 1227 1524 1605 1740
Size: 279 818 130 297 81 135
Fragments arranged by size:
818 297 279 135 130 81
BtsI GCAGTG_nn'
Cuts at: 0 1249 1740
Size: 1249 491
DraIII CAC_nnn'GTG
Cuts at: 0 802 1740
Size: 802 938
DrdI GACnn_nn'nnGTC
Cuts at: 0 910 1740
Size: 910 830
EaeI y'GGCC_r
Cuts at: 0 530 1740
Size: 530 1210
EarI CTCTTCn'nnn_
Cuts at: 0 104 278 316 1480 1516 1540 1740
Size: 104 174 38 1164 36 24 200
Fragments arranged by size:
1164 200 174 104 38 36 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 596 822 1648 1740
Size: 596 226 826 92
Fragments arranged by size:
826 596 226 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 630 709 730 750 844 1740
Size: 630 79 21 20 94 896
Fragments arranged by size:
896 630 94 79 21 20
EcoO109I rG'GnC_Cy
Cuts at: 0 68 79 1342 1343 1707 1740
Size: 68 11 1263 1 364 33
Fragments arranged by size:
1263 364 68 33 11 1
HaeII r_GCGC'y
Cuts at: 0 1559 1740
Size: 1559 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1007 1039 1740
Size: 1007 32 701
Fragments arranged by size:
1007 701 32
KasI G'GCGC_C
Cuts at: 0 1555 1740
Size: 1555 185
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 327 1740
Size: 121 206 1413
Fragments arranged by size:
1413 206 121
MslI CAynn'nnrTG
Cuts at: 0 580 860 1108 1420 1519 1740
Size: 580 280 248 312 99 221
Fragments arranged by size:
580 312 280 248 221 99
MspA1I CmG'CkG
Cuts at: 0 264 372 501 1011 1664 1740
Size: 264 108 129 510 653 76
Fragments arranged by size:
653 510 264 129 108 76
NarI GG'CG_CC
Cuts at: 0 1556 1740
Size: 1556 184
NcoI C'CATG_G
Cuts at: 0 1373 1740
Size: 1373 367
NspI r_CATG'y
Cuts at: 0 1419 1740
Size: 1419 321
PciI A'CATG_T
Cuts at: 0 1415 1740
Size: 1415 325
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 408 681 1740
Size: 396 12 273 1059
Fragments arranged by size:
1059 396 273 12
PfoI T'CCnGG_A
Cuts at: 0 278 749 842 1740
Size: 278 471 93 898
Fragments arranged by size:
898 471 278 93
PmlI CAC'GTG
Cuts at: 0 522 1740
Size: 522 1218
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1375 1407 1740
Size: 1375 32 333
Fragments arranged by size:
1375 333 32
PpuMI rG'GwC_Cy
Cuts at: 0 68 1707 1740
Size: 68 1639 33
Fragments arranged by size:
1639 68 33
PspOMI G'GGCC_C
Cuts at: 0 1342 1740
Size: 1342 398
PstI C_TGCA'G
Cuts at: 0 980 1159 1613 1684 1740
Size: 980 179 454 71 56
Fragments arranged by size:
980 454 179 71 56
PvuII CAG'CTG
Cuts at: 0 264 372 1740
Size: 264 108 1368
Fragments arranged by size:
1368 264 108
SacII CC_GC'GG
Cuts at: 0 1665 1740
Size: 1665 75
SanDI GG'GwC_CC
Cuts at: 0 68 1707 1740
Size: 68 1639 33
Fragments arranged by size:
1639 68 33
SapI GCTCTTCn'nnn_
Cuts at: 0 1480 1740
Size: 1480 260
SfcI C'TryA_G
Cuts at: 0 976 1155 1609 1680 1740
Size: 976 179 454 71 60
Fragments arranged by size:
976 454 179 71 60
SfoI GGC'GCC
Cuts at: 0 1557 1740
Size: 1557 183
SgrAI Cr'CCGG_yG
Cuts at: 0 1552 1740
Size: 1552 188
SmaI CCC'GGG
Cuts at: 0 60 1740
Size: 60 1680
StuI AGG'CCT
Cuts at: 0 1615 1740
Size: 1615 125
StyI C'CwwG_G
Cuts at: 0 173 466 1373 1740
Size: 173 293 907 367
Fragments arranged by size:
907 367 293 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 1182 1740
Size: 49 1133 558
Fragments arranged by size:
1133 558 49
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1254 1740
Size: 1254 486
XmaI C'CCGG_G
Cuts at: 0 58 1740
Size: 58 1682
XmnI GAAnn'nnTTC
Cuts at: 0 119 1740
Size: 119 1621
ZraI GAC'GTC
Cuts at: 0 1152 1740
Size: 1152 588
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BclI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI
MmeI MslI MspA1I NarI NcoI NspI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacII
SanDI SapI SfcI SfoI SgrAI SmaI StuI StyI
TaqII XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BciVI BfrBI BglII BmtI BplI BpuEI BsaWI BsiEI
BsiWI BspEI BsrDI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I ClaI CspCI DraI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI
SexAI SfiI SgfI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XhoI