Restriction map for
pFN21AE4523
(Linear) (Six-base) MAPSORT of: KIEE4523.seq Check: 3601 from: 1 to: 1689
KIEE4523.orf
With 182 enzymes: SgfI *
March 22, 2012 18:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 555 1313 1689
Size: 555 758 376
Fragments arranged by size:
758 555 376
AccI GT'mk_AC
Cuts at: 0 872 1689
Size: 872 817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1218 1689
Size: 1218 471
AflIII A'CryG_T
Cuts at: 0 954 1689
Size: 954 735
AleI CACnn'nnGTG
Cuts at: 0 1159 1689
Size: 1159 530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1411 1689
Size: 1411 278
AlwNI CAG_nnn'CTG
Cuts at: 0 577 690 837 1689
Size: 577 113 147 852
Fragments arranged by size:
852 577 147 113
AvaI C'yCGr_G
Cuts at: 0 1046 1689
Size: 1046 643
AvrII C'CTAG_G
Cuts at: 0 21 1689
Size: 21 1668
BamHI G'GATC_C
Cuts at: 0 207 1448 1475 1626 1689
Size: 207 1241 27 151 63
Fragments arranged by size:
1241 207 151 63 27
BanI G'GyrC_C
Cuts at: 0 655 850 1689
Size: 655 195 839
Fragments arranged by size:
839 655 195
BanII G_rGCy'C
Cuts at: 0 29 74 324 652 908 1031 1294 1689
Size: 29 45 250 328 256 123 263 395
Fragments arranged by size:
395 328 263 256 250 123 45 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1116 1678 1689
Size: 1116 562 11
Fragments arranged by size:
1116 562 11
BbvCI CC'TCA_GC
Cuts at: 0 994 1689
Size: 994 695
BclI T'GATC_A
Cuts at: 0 341 1460 1689
Size: 341 1119 229
Fragments arranged by size:
1119 341 229
BlpI GC'TnA_GC
Cuts at: 0 370 1689
Size: 370 1319
Bme1580I G_kGCm'C
Cuts at: 0 684 1400 1689
Size: 684 716 289
Fragments arranged by size:
716 684 289
BmgBI CAC'GTC
Cuts at: 0 645 1689
Size: 645 1044
BmrI ACTGGGnnnn_n'
Cuts at: 0 678 1386 1689
Size: 678 708 303
Fragments arranged by size:
708 678 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1125 1418 1689
Size: 712 413 293 271
Fragments arranged by size:
712 413 293 271
Bpu10I CC'TnA_GC
Cuts at: 0 557 994 1288 1689
Size: 557 437 294 401
Fragments arranged by size:
557 437 401 294
BsaI GGTCTCn'nnnn_
Cuts at: 0 1057 1689
Size: 1057 632
BsaWI w'CCGG_w
Cuts at: 0 1414 1689
Size: 1414 275
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 269 299 1689
Size: 269 30 1390
Fragments arranged by size:
1390 269 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 209 1689
Size: 209 1480
BseYI C'CCAG_C
Cuts at: 0 101 211 1622 1640 1659 1689
Size: 101 110 1411 18 19 30
Fragments arranged by size:
1411 110 101 30 19 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 502 850 1343 1689
Size: 214 288 348 493 346
Fragments arranged by size:
493 348 346 288 214
BsiHKAI G_wGCw'C
Cuts at: 0 1031 1294 1472 1689
Size: 1031 263 178 217
Fragments arranged by size:
1031 263 217 178
Bsp1286I G_dGCh'C
Cuts at: 0 29 74 324 652 684 908 1031 1294
Size: 29 45 250 328 32 224 123 263
Cuts at: 1294 1400 1472 1689
Size: 106 72 217
Fragments arranged by size:
328 263 250 224 217 123 106 72
45 32 29
BspEI T'CCGG_A
Cuts at: 0 1414 1689
Size: 1414 275
BspHI T'CATG_A
Cuts at: 0 1031 1689
Size: 1031 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 555 1313 1689
Size: 555 758 376
Fragments arranged by size:
758 555 376
BsrBI CCG'CTC
Cuts at: 0 1045 1689
Size: 1045 644
BsrDI GCAATG_nn'
Cuts at: 0 1245 1689
Size: 1245 444
BsrFI r'CCGG_y
Cuts at: 0 887 1689
Size: 887 802
BssSI C'ACGA_G
Cuts at: 0 1114 1689
Size: 1114 575
BstEII G'GTnAC_C
Cuts at: 0 597 1689
Size: 597 1092
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1531 1689
Size: 1531 158
BstYI r'GATC_y
Cuts at: 0 207 1448 1475 1626 1689
Size: 207 1241 27 151 63
Fragments arranged by size:
1241 207 151 63 27
Bsu36I CC'TnA_GG
Cuts at: 0 128 544 1071 1689
Size: 128 416 527 618
Fragments arranged by size:
618 527 416 128
BtgI C'CryG_G
Cuts at: 0 1249 1405 1557 1689
Size: 1249 156 152 132
Fragments arranged by size:
1249 156 152 132
BtsI GCAGTG_nn'
Cuts at: 0 922 1689
Size: 922 767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 467 502 1314 1349 1689
Size: 467 35 812 35 340
Fragments arranged by size:
812 467 340 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 294 423 1689
Size: 294 129 1266
Fragments arranged by size:
1266 294 129
EaeI y'GGCC_r
Cuts at: 0 885 1335 1485 1689
Size: 885 450 150 204
Fragments arranged by size:
885 450 204 150
EarI CTCTTCn'nnn_
Cuts at: 0 128 742 1069 1580 1689
Size: 128 614 327 511 109
Fragments arranged by size:
614 511 327 128 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1111 1689
Size: 1111 578
EcoICRI GAG'CTC
Cuts at: 0 1029 1292 1689
Size: 1029 263 397
Fragments arranged by size:
1029 397 263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1125 1218 1418 1689
Size: 712 413 93 200 271
Fragments arranged by size:
712 413 271 200 93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1634 1689
Size: 1634 55
EcoO109I rG'GnC_Cy
Cuts at: 0 430 455 1689
Size: 430 25 1234
Fragments arranged by size:
1234 430 25
HaeII r_GCGC'y
Cuts at: 0 1004 1689
Size: 1004 685
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 537 569 634 666 973 1005
Size: 199 32 306 32 65 32 307 32
Cuts at: 1005 1689
Size: 684
Fragments arranged by size:
684 307 306 199 65 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1020 1212 1689
Size: 1020 192 477
Fragments arranged by size:
1020 477 192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 402 762 1689
Size: 185 217 360 927
Fragments arranged by size:
927 360 217 185
MslI CAynn'nnrTG
Cuts at: 0 419 1159 1689
Size: 419 740 530
Fragments arranged by size:
740 530 419
MspA1I CmG'CkG
Cuts at: 0 81 1014 1689
Size: 81 933 675
Fragments arranged by size:
933 675 81
NaeI GCC'GGC
Cuts at: 0 889 1689
Size: 889 800
NcoI C'CATG_G
Cuts at: 0 1405 1689
Size: 1405 284
NgoMIV G'CCGG_C
Cuts at: 0 887 1689
Size: 887 802
NspI r_CATG'y
Cuts at: 0 958 1689
Size: 958 731
PasI CC'CwG_GG
Cuts at: 0 111 400 1689
Size: 111 289 1289
Fragments arranged by size:
1289 289 111
PciI A'CATG_T
Cuts at: 0 954 1689
Size: 954 735
PflMI CCAn_nnn'nTGG
Cuts at: 0 690 1406 1441 1689
Size: 690 716 35 248
Fragments arranged by size:
716 690 248 35
PfoI T'CCnGG_A
Cuts at: 0 689 1178 1689
Size: 689 489 511
Fragments arranged by size:
689 511 489
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1175 1207 1689
Size: 1175 32 482
Fragments arranged by size:
1175 482 32
PpuMI rG'GwC_Cy
Cuts at: 0 430 455 1689
Size: 430 25 1234
Fragments arranged by size:
1234 430 25
PstI C_TGCA'G
Cuts at: 0 917 1689
Size: 917 772
SacI G_AGCT'C
Cuts at: 0 1031 1294 1689
Size: 1031 263 395
Fragments arranged by size:
1031 395 263
SapI GCTCTTCn'nnn_
Cuts at: 0 128 1069 1689
Size: 128 941 620
Fragments arranged by size:
941 620 128
SfcI C'TryA_G
Cuts at: 0 578 873 913 1689
Size: 578 295 40 776
Fragments arranged by size:
776 578 295 40
StuI AGG'CCT
Cuts at: 0 484 584 1689
Size: 484 100 1105
Fragments arranged by size:
1105 484 100
StyI C'CwwG_G
Cuts at: 0 21 485 1405 1513 1689
Size: 21 464 920 108 176
Fragments arranged by size:
920 464 176 108 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 93 1368 1398 1665 1689
Size: 93 1275 30 267 24
Fragments arranged by size:
1275 267 93 30 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 1467 1499 1640 1672 1689
Size: 269 32 1166 32 141 32 17
Fragments arranged by size:
1166 269 141 32 32 32 17
XbaI T'CTAG_A
Cuts at: 0 822 1689
Size: 822 867
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BclI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MmeI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PpiI PpuMI PstI SacI SapI SfcI StuI
StyI TaqII TstI XbaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI ApoI AscI AseI AsiSI BaeI BbeI
BcgI BciVI BfrBI BglI BglII BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BsrGI
BssHII BstAPI BstBI BstZ17I BtgZI ClaI DraI DrdI
EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NdeI
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XcmI XhoI XmaI XmnI ZraI