Restriction map for
pFN21AE4521
(Linear) (Six-base) MAPSORT of: KIEE4521.seq Check: 5442 from: 1 to: 1671
KIEE4521.orf
With 182 enzymes: SgfI *
March 22, 2012 18:15 ..
AatII G_ACGT'C
Cuts at: 0 368 1671
Size: 368 1303
AccI GT'mk_AC
Cuts at: 0 368 1337 1671
Size: 368 969 334
Fragments arranged by size:
969 368 334
Acc65I G'GTAC_C
Cuts at: 0 1257 1540 1671
Size: 1257 283 131
Fragments arranged by size:
1257 283 131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1671
Size: 531 1140
AflIII A'CryG_T
Cuts at: 0 1340 1671
Size: 1340 331
AleI CACnn'nnGTG
Cuts at: 0 382 819 1369 1671
Size: 382 437 550 302
Fragments arranged by size:
550 437 382 302
AlwNI CAG_nnn'CTG
Cuts at: 0 182 1671
Size: 182 1489
ApaI G_GGCC'C
Cuts at: 0 47 1671
Size: 47 1624
ApoI r'AATT_y
Cuts at: 0 573 659 1671
Size: 573 86 1012
Fragments arranged by size:
1012 573 86
AvaI C'yCGr_G
Cuts at: 0 942 1671
Size: 942 729
BanI G'GyrC_C
Cuts at: 0 153 228 1257 1450 1540 1671
Size: 153 75 1029 193 90 131
Fragments arranged by size:
1029 193 153 131 90 75
BanII G_rGCy'C
Cuts at: 0 47 469 1671
Size: 47 422 1202
Fragments arranged by size:
1202 422 47
BciVI GTATCCnnnnn_n'
Cuts at: 0 1370 1671
Size: 1370 301
BclI T'GATC_A
Cuts at: 0 884 1671
Size: 884 787
BfrBI ATG'CAT
Cuts at: 0 718 1671
Size: 718 953
BglII A'GATC_T
Cuts at: 0 560 620 1671
Size: 560 60 1051
Fragments arranged by size:
1051 560 60
BlpI GC'TnA_GC
Cuts at: 0 73 1671
Size: 73 1598
Bme1580I G_kGCm'C
Cuts at: 0 47 233 1671
Size: 47 186 1438
Fragments arranged by size:
1438 186 47
BmgBI CAC'GTC
Cuts at: 0 306 1671
Size: 306 1365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 343 1671
Size: 310 33 1328
Fragments arranged by size:
1328 310 33
Bpu10I CC'TnA_GC
Cuts at: 0 172 1671
Size: 172 1499
BsaAI yAC'GTr
Cuts at: 0 1341 1671
Size: 1341 330
BsaHI Gr'CG_yC
Cuts at: 0 161 365 1671
Size: 161 204 1306
Fragments arranged by size:
1306 204 161
BsaWI w'CCGG_w
Cuts at: 0 339 1019 1671
Size: 339 680 652
Fragments arranged by size:
680 652 339
BseYI C'CCAG_C
Cuts at: 0 347 1152 1671
Size: 347 805 519
Fragments arranged by size:
805 519 347
BsiEI CG_ry'CG
Cuts at: 0 222 1461 1671
Size: 222 1239 210
Fragments arranged by size:
1239 222 210
BsiHKAI G_wGCw'C
Cuts at: 0 143 1671
Size: 143 1528
Bsp1286I G_dGCh'C
Cuts at: 0 47 143 233 469 1671
Size: 47 96 90 236 1202
Fragments arranged by size:
1202 236 96 90 47
BspEI T'CCGG_A
Cuts at: 0 339 1019 1671
Size: 339 680 652
Fragments arranged by size:
680 652 339
BsrFI r'CCGG_y
Cuts at: 0 103 251 1671
Size: 103 148 1420
Fragments arranged by size:
1420 148 103
BsrGI T'GTAC_A
Cuts at: 0 1343 1671
Size: 1343 328
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1188 1671
Size: 1188 483
BstBI TT'CG_AA
Cuts at: 0 1005 1671
Size: 1005 666
BstEII G'GTnAC_C
Cuts at: 0 18 1026 1671
Size: 18 1008 645
Fragments arranged by size:
1008 645 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 633 1065 1671
Size: 633 432 606
Fragments arranged by size:
633 606 432
BstYI r'GATC_y
Cuts at: 0 560 620 996 1671
Size: 560 60 376 675
Fragments arranged by size:
675 560 376 60
BtgI C'CryG_G
Cuts at: 0 527 1205 1331 1671
Size: 527 678 126 340
Fragments arranged by size:
678 527 340 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 249 1153 1671
Size: 249 904 518
Fragments arranged by size:
904 518 249
BtsI GCAGTG_nn'
Cuts at: 0 202 1211 1239 1671
Size: 202 1009 28 432
Fragments arranged by size:
1009 432 202 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 918 953 1671
Size: 918 35 718
Fragments arranged by size:
918 718 35
EaeI y'GGCC_r
Cuts at: 0 219 501 1208 1671
Size: 219 282 707 463
Fragments arranged by size:
707 463 282 219
EagI C'GGCC_G
Cuts at: 0 219 1671
Size: 219 1452
EarI CTCTTCn'nnn_
Cuts at: 0 866 1225 1249 1671
Size: 866 359 24 422
Fragments arranged by size:
866 422 359 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 783 1671
Size: 783 888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 343 531 1671
Size: 310 33 188 1140
Fragments arranged by size:
1140 310 188 33
EcoO109I rG'GnC_Cy
Cuts at: 0 43 44 423 1671
Size: 43 1 379 1248
Fragments arranged by size:
1248 379 43 1
HaeII r_GCGC'y
Cuts at: 0 57 80 182 283 787 1200 1671
Size: 57 23 102 101 504 413 471
Fragments arranged by size:
504 471 413 102 101 57 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 836 868 1671
Size: 836 32 803
Fragments arranged by size:
836 803 32
KpnI G_GTAC'C
Cuts at: 0 1261 1544 1671
Size: 1261 283 127
Fragments arranged by size:
1261 283 127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1671
Size: 650 1021
MscI TGG'CCA
Cuts at: 0 1210 1671
Size: 1210 461
MslI CAynn'nnrTG
Cuts at: 0 382 532 819 1033 1063 1117 1369 1384
Size: 382 150 287 214 30 54 252 15
Cuts at: 1384 1480 1618 1671
Size: 96 138 53
Fragments arranged by size:
382 287 252 214 150 138 96 54
53 30 15
MspA1I CmG'CkG
Cuts at: 0 146 239 321 486 1254 1671
Size: 146 93 82 165 768 417
Fragments arranged by size:
768 417 165 146 93 82
NcoI C'CATG_G
Cuts at: 0 527 1205 1331 1671
Size: 527 678 126 340
Fragments arranged by size:
678 527 340 126
NdeI CA'TA_TG
Cuts at: 0 1158 1671
Size: 1158 513
NsiI A_TGCA'T
Cuts at: 0 720 1671
Size: 720 951
NspI r_CATG'y
Cuts at: 0 537 1671
Size: 537 1134
PspOMI G'GGCC_C
Cuts at: 0 43 1671
Size: 43 1628
PstI C_TGCA'G
Cuts at: 0 560 1671
Size: 560 1111
PvuII CAG'CTG
Cuts at: 0 239 321 486 1254 1671
Size: 239 82 165 768 417
Fragments arranged by size:
768 417 239 165 82
SexAI A'CCwGG_T
Cuts at: 0 398 1671
Size: 398 1273
SfcI C'TryA_G
Cuts at: 0 556 1283 1671
Size: 556 727 388
Fragments arranged by size:
727 556 388
SphI G_CATG'C
Cuts at: 0 537 1671
Size: 537 1134
SspI AAT'ATT
Cuts at: 0 686 1186 1671
Size: 686 500 485
Fragments arranged by size:
686 500 485
StyI C'CwwG_G
Cuts at: 0 527 1205 1331 1446 1671
Size: 527 678 126 115 225
Fragments arranged by size:
678 527 225 126 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 403 442 1216 1372 1671
Size: 403 39 774 156 299
Fragments arranged by size:
774 403 299 156 39
TatI w'GTAC_w
Cuts at: 0 1343 1671
Size: 1343 328
XmnI GAAnn'nnTTC
Cuts at: 0 1126 1671
Size: 1126 545
ZraI GAC'GTC
Cuts at: 0 366 1671
Size: 366 1305
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AlwNI ApaI
ApoI AvaI BanI BanII BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BsrFI BsrGI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I
KpnI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PspOMI PstI PvuII SexAI SfcI SphI SspI
StyI TaqII TatI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BglI BmrI BmtI BplI BpuEI
BsaI BsaBI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BspHI BspMI BsrBI BsrDI BssHII BssSI BstZ17I Bsu36I
ClaI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI