Restriction map for
pFN21AE4515
(Linear) (Six-base) MAPSORT of: KIEE4515.seq Check: 7969 from: 1 to: 1641
KIEE4515.orf
With 182 enzymes: SgfI *
March 22, 2012 18:15 ..
AccI GT'mk_AC
Cuts at: 0 104 430 1641
Size: 104 326 1211
Fragments arranged by size:
1211 326 104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 668 692 721 1231 1641
Size: 26 642 24 29 510 410
Fragments arranged by size:
642 510 410 29 26 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1032 1641
Size: 1032 609
AlwNI CAG_nnn'CTG
Cuts at: 0 699 1641
Size: 699 942
ApoI r'AATT_y
Cuts at: 0 399 1060 1141 1641
Size: 399 661 81 500
Fragments arranged by size:
661 500 399 81
AvaI C'yCGr_G
Cuts at: 0 835 1641
Size: 835 806
BamHI G'GATC_C
Cuts at: 0 751 1641
Size: 751 890
BanI G'GyrC_C
Cuts at: 0 117 1349 1622 1641
Size: 117 1232 273 19
Fragments arranged by size:
1232 273 117 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 837 1527 1641
Size: 837 690 114
Fragments arranged by size:
837 690 114
BbvCI CC'TCA_GC
Cuts at: 0 1552 1641
Size: 1552 89
BfrBI ATG'CAT
Cuts at: 0 1243 1641
Size: 1243 398
BglI GCCn_nnn'nGGC
Cuts at: 0 703 1495 1641
Size: 703 792 146
Fragments arranged by size:
792 703 146
BlpI GC'TnA_GC
Cuts at: 0 1093 1641
Size: 1093 548
Bme1580I G_kGCm'C
Cuts at: 0 1352 1641
Size: 1352 289
BmrI ACTGGGnnnn_n'
Cuts at: 0 750 1641
Size: 750 891
BmtI G_CTAG'C
Cuts at: 0 817 1641
Size: 817 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 579 728 1641
Size: 46 533 149 913
Fragments arranged by size:
913 533 149 46
Bpu10I CC'TnA_GC
Cuts at: 0 1552 1641
Size: 1552 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 162 1641
Size: 19 143 1479
Fragments arranged by size:
1479 143 19
BsaAI yAC'GTr
Cuts at: 0 189 1304 1641
Size: 189 1115 337
Fragments arranged by size:
1115 337 189
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1232 1641
Size: 1232 409
BsiHKAI G_wGCw'C
Cuts at: 0 148 1099 1641
Size: 148 951 542
Fragments arranged by size:
951 542 148
BsmI GAATG_Cn'
Cuts at: 0 379 1116 1243 1641
Size: 379 737 127 398
Fragments arranged by size:
737 398 379 127
Bsp1286I G_dGCh'C
Cuts at: 0 148 1099 1352 1641
Size: 148 951 253 289
Fragments arranged by size:
951 289 253 148
BspHI T'CATG_A
Cuts at: 0 597 1641
Size: 597 1044
BsrDI GCAATG_nn'
Cuts at: 0 665 824 1641
Size: 665 159 817
Fragments arranged by size:
817 665 159
BsrGI T'GTAC_A
Cuts at: 0 797 1375 1641
Size: 797 578 266
Fragments arranged by size:
797 578 266
BstAPI GCAn_nnn'nTGC
Cuts at: 0 823 1641
Size: 823 818
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 1641
Size: 792 849
BstYI r'GATC_y
Cuts at: 0 751 1641
Size: 751 890
BstZ17I GTA'TAC
Cuts at: 0 431 1641
Size: 431 1210
Bsu36I CC'TnA_GG
Cuts at: 0 1070 1641
Size: 1070 571
BtgI C'CryG_G
Cuts at: 0 14 1641
Size: 14 1627
BtsI GCAGTG_nn'
Cuts at: 0 860 1641
Size: 860 781
DrdI GACnn_nn'nnGTC
Cuts at: 0 787 1641
Size: 787 854
EaeI y'GGCC_r
Cuts at: 0 11 1641
Size: 11 1630
EarI CTCTTCn'nnn_
Cuts at: 0 339 644 1207 1261 1641
Size: 339 305 563 54 380
Fragments arranged by size:
563 380 339 305 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 46 579 668 692 721 728 1231
Size: 26 20 533 89 24 29 7 503
Cuts at: 1231 1641
Size: 410
Fragments arranged by size:
533 503 410 89 29 26 24 20
7
EcoO109I rG'GnC_Cy
Cuts at: 0 696 1641
Size: 696 945
EcoRI G'AATT_C
Cuts at: 0 1141 1641
Size: 1141 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 1048 1063 1080 1095 1317 1349
Size: 316 32 700 15 17 15 222 32
Cuts at: 1349 1641
Size: 292
Fragments arranged by size:
700 316 292 222 32 32 17 15
15
HincII GTy'rAC
Cuts at: 0 324 1641
Size: 324 1317
HindIII A'AGCT_T
Cuts at: 0 993 1182 1544 1641
Size: 993 189 362 97
Fragments arranged by size:
993 362 189 97
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 1641
Size: 362 1279
MslI CAynn'nnrTG
Cuts at: 0 1489 1641
Size: 1489 152
MspA1I CmG'CkG
Cuts at: 0 1027 1641
Size: 1027 614
NdeI CA'TA_TG
Cuts at: 0 951 1239 1641
Size: 951 288 402
Fragments arranged by size:
951 402 288
NheI G'CTAG_C
Cuts at: 0 813 1641
Size: 813 828
NsiI A_TGCA'T
Cuts at: 0 1245 1641
Size: 1245 396
NspI r_CATG'y
Cuts at: 0 159 1641
Size: 159 1482
PflMI CCAn_nnn'nTGG
Cuts at: 0 1619 1641
Size: 1619 22
PfoI T'CCnGG_A
Cuts at: 0 1517 1641
Size: 1517 124
PstI C_TGCA'G
Cuts at: 0 594 1641
Size: 594 1047
PvuII CAG'CTG
Cuts at: 0 1027 1641
Size: 1027 614
SfcI C'TryA_G
Cuts at: 0 590 1641
Size: 590 1051
SmlI C'TyrA_G
Cuts at: 0 34 141 1641
Size: 34 107 1500
Fragments arranged by size:
1500 107 34
StyI C'CwwG_G
Cuts at: 0 605 1337 1641
Size: 605 732 304
Fragments arranged by size:
732 605 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 426 1406 1641
Size: 426 980 235
Fragments arranged by size:
980 426 235
TatI w'GTAC_w
Cuts at: 0 797 1375 1641
Size: 797 578 266
Fragments arranged by size:
797 578 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1499 1531 1641
Size: 1499 32 110
Fragments arranged by size:
1499 110 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 21 1616 1641
Size: 21 1595 25
Fragments arranged by size:
1595 25 21
XmnI GAAnn'nnTTC
Cuts at: 0 883 932 1641
Size: 883 49 709
Fragments arranged by size:
883 709 49
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApoI AvaI BamHI BanI
BbsI BbvCI BfrBI BglI BlpI Bme1580I BmrI BmtI
BpmI Bpu10I BpuEI BsaAI BseRI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BsrGI BstAPI BstXI BstYI BstZ17I Bsu36I
BtgI BtsI DrdI EaeI EarI Eco57MI EcoO109I EcoRI
Hin4I HincII HindIII MmeI MslI MspA1I NdeI NheI
NsiI NspI PflMI PfoI PstI PvuII SfcI SmlI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanII BbeI BcgI BciVI BclI BglII
BmgBI BplI BsaI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BssHII BssSI BstBI BstEII BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NgoMIV NotI NruI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI