Restriction map for
pFN21AE4507
(Linear) (Six-base) MAPSORT of: KIEE4507.seq Check: 7834 from: 1 to: 1590
KIEE4507.orf
With 182 enzymes: SgfI *
March 22, 2012 18:13 ..
AatII G_ACGT'C
Cuts at: 0 19 1590
Size: 19 1571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1590
Size: 709 881
AflIII A'CryG_T
Cuts at: 0 947 1590
Size: 947 643
ApoI r'AATT_y
Cuts at: 0 275 677 796 1343 1531 1590
Size: 275 402 119 547 188 59
Fragments arranged by size:
547 402 275 188 119 59
AseI AT'TA_AT
Cuts at: 0 1301 1590
Size: 1301 289
BamHI G'GATC_C
Cuts at: 0 1163 1457 1590
Size: 1163 294 133
Fragments arranged by size:
1163 294 133
BanII G_rGCy'C
Cuts at: 0 898 1590
Size: 898 692
BbsI GAAGACnn'nnnn_
Cuts at: 0 124 508 732 1377 1590
Size: 124 384 224 645 213
Fragments arranged by size:
645 384 224 213 124
BclI T'GATC_A
Cuts at: 0 609 938 1590
Size: 609 329 652
Fragments arranged by size:
652 609 329
BfrBI ATG'CAT
Cuts at: 0 1285 1590
Size: 1285 305
BglI GCCn_nnn'nGGC
Cuts at: 0 1504 1590
Size: 1504 86
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1255 1294 1590
Size: 1255 39 296
Fragments arranged by size:
1255 296 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1590
Size: 920 670
BsaBI GATnn'nnATC
Cuts at: 0 1519 1590
Size: 1519 71
BsaHI Gr'CG_yC
Cuts at: 0 16 1590
Size: 16 1574
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1590
Size: 910 680
BsiHKAI G_wGCw'C
Cuts at: 0 898 1590
Size: 898 692
BsmI GAATG_Cn'
Cuts at: 0 1178 1486 1590
Size: 1178 308 104
Fragments arranged by size:
1178 308 104
Bsp1286I G_dGCh'C
Cuts at: 0 898 1590
Size: 898 692
BspHI T'CATG_A
Cuts at: 0 150 1328 1350 1590
Size: 150 1178 22 240
Fragments arranged by size:
1178 240 150 22
BsrDI GCAATG_nn'
Cuts at: 0 1208 1590
Size: 1208 382
BsrGI T'GTAC_A
Cuts at: 0 509 1061 1590
Size: 509 552 529
Fragments arranged by size:
552 529 509
BstBI TT'CG_AA
Cuts at: 0 777 1590
Size: 777 813
BstEII G'GTnAC_C
Cuts at: 0 1070 1590
Size: 1070 520
BstYI r'GATC_y
Cuts at: 0 992 1163 1457 1590
Size: 992 171 294 133
Fragments arranged by size:
992 294 171 133
Bsu36I CC'TnA_GG
Cuts at: 0 869 1590
Size: 869 721
BtgI C'CryG_G
Cuts at: 0 1158 1590
Size: 1158 432
EarI CTCTTCn'nnn_
Cuts at: 0 125 272 875 1590
Size: 125 147 603 715
Fragments arranged by size:
715 603 147 125
EcoICRI GAG'CTC
Cuts at: 0 896 1590
Size: 896 694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 920 1590
Size: 709 211 670
Fragments arranged by size:
709 670 211
EcoNI CCTnn'n_nnAGG
Cuts at: 0 868 1590
Size: 868 722
EcoRI G'AATT_C
Cuts at: 0 1343 1590
Size: 1343 247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 283 1590
Size: 283 1307
HincII GTy'rAC
Cuts at: 0 167 1590
Size: 167 1423
HindIII A'AGCT_T
Cuts at: 0 1109 1555 1590
Size: 1109 446 35
Fragments arranged by size:
1109 446 35
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 1590
Size: 813 777
NcoI C'CATG_G
Cuts at: 0 1158 1590
Size: 1158 432
NdeI CA'TA_TG
Cuts at: 0 650 1590
Size: 650 940
NsiI A_TGCA'T
Cuts at: 0 1287 1590
Size: 1287 303
NspI r_CATG'y
Cuts at: 0 951 1590
Size: 951 639
PciI A'CATG_T
Cuts at: 0 947 1590
Size: 947 643
PflMI CCAn_nnn'nTGG
Cuts at: 0 1473 1590
Size: 1473 117
PfoI T'CCnGG_A
Cuts at: 0 125 1590
Size: 125 1465
PshAI GACnn'nnGTC
Cuts at: 0 19 1590
Size: 19 1571
PsiI TTA'TAA
Cuts at: 0 1117 1590
Size: 1117 473
SacI G_AGCT'C
Cuts at: 0 898 1590
Size: 898 692
SapI GCTCTTCn'nnn_
Cuts at: 0 125 1590
Size: 125 1465
SfcI C'TryA_G
Cuts at: 0 366 1590
Size: 366 1224
SspI AAT'ATT
Cuts at: 0 358 1590
Size: 358 1232
StyI C'CwwG_G
Cuts at: 0 1158 1226 1590
Size: 1158 68 364
Fragments arranged by size:
1158 364 68
TatI w'GTAC_w
Cuts at: 0 509 1061 1590
Size: 509 552 529
Fragments arranged by size:
552 529 509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 282 314 1590
Size: 282 32 1276
Fragments arranged by size:
1276 282 32
Tth111I GACn'n_nGTC
Cuts at: 0 1260 1309 1590
Size: 1260 49 281
Fragments arranged by size:
1260 281 49
ZraI GAC'GTC
Cuts at: 0 17 1590
Size: 17 1573
Enzymes that do cut:
AatII AcuI AflIII ApoI AseI BamHI BanII BbsI
BclI BfrBI BglI BplI BpmI BsaBI BsaHI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstBI BstEII
BstYI Bsu36I BtgI EarI EcoICRI Eco57MI EcoNI EcoRI
FalI HincII HindIII MmeI NcoI NdeI NsiI NspI
PciI PflMI PfoI PshAI PsiI SacI SapI SfcI
SspI StyI TatI TstI Tth111I ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BglII BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoO109I EcoRV FseI FspI
FspAI HaeII Hin4I HpaI KasI KpnI MfeI MluI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TaqII XbaI XcmI XhoI XmaI XmnI