Restriction map for
pFN21AE4505
(Linear) (Six-base) MAPSORT of: KIEE4505.seq Check: 2124 from: 1 to: 1587
KIEE4505.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 988 1587
Size: 988 599
Acc65I G'GTAC_C
Cuts at: 0 1259 1330 1587
Size: 1259 71 257
Fragments arranged by size:
1259 257 71
AclI AA'CG_TT
Cuts at: 0 1494 1587
Size: 1494 93
AfeI AGC'GCT
Cuts at: 0 106 199 1587
Size: 106 93 1388
Fragments arranged by size:
1388 106 93
AflIII A'CryG_T
Cuts at: 0 596 1587
Size: 596 991
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1258 1290 1587
Size: 1258 32 297
Fragments arranged by size:
1258 297 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1074 1418 1435 1587
Size: 1074 344 17 152
Fragments arranged by size:
1074 344 152 17
ApaI G_GGCC'C
Cuts at: 0 1242 1587
Size: 1242 345
BanI G'GyrC_C
Cuts at: 0 853 1259 1330 1587
Size: 853 406 71 257
Fragments arranged by size:
853 406 257 71
BanII G_rGCy'C
Cuts at: 0 405 662 848 1242 1587
Size: 405 257 186 394 345
Fragments arranged by size:
405 394 345 257 186
BbsI GAAGACnn'nnnn_
Cuts at: 0 924 1587
Size: 924 663
BbvCI CC'TCA_GC
Cuts at: 0 449 1522 1587
Size: 449 1073 65
Fragments arranged by size:
1073 449 65
BciVI GTATCCnnnnn_n'
Cuts at: 0 516 760 817 1587
Size: 516 244 57 770
Fragments arranged by size:
770 516 244 57
Bme1580I G_kGCm'C
Cuts at: 0 457 1242 1587
Size: 457 785 345
Fragments arranged by size:
785 457 345
BmrI ACTGGGnnnn_n'
Cuts at: 0 869 1587
Size: 869 718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 436 1072 1587
Size: 289 147 636 515
Fragments arranged by size:
636 515 289 147
Bpu10I CC'TnA_GC
Cuts at: 0 449 1337 1522 1587
Size: 449 888 185 65
Fragments arranged by size:
888 449 185 65
BsaAI yAC'GTr
Cuts at: 0 1305 1587
Size: 1305 282
BsaBI GATnn'nnATC
Cuts at: 0 944 1587
Size: 944 643
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 953 983 1373 1403 1587
Size: 953 30 390 30 184
Fragments arranged by size:
953 390 184 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 974 1587
Size: 370 604 613
Fragments arranged by size:
613 604 370
BsiEI CG_ry'CG
Cuts at: 0 158 1587
Size: 158 1429
BsiHKAI G_wGCw'C
Cuts at: 0 405 662 907 1587
Size: 405 257 245 680
Fragments arranged by size:
680 405 257 245
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1092 1587
Size: 1092 495
Bsp1286I G_dGCh'C
Cuts at: 0 405 457 662 848 907 1242 1587
Size: 405 52 205 186 59 335 345
Fragments arranged by size:
405 345 335 205 186 59 52
BspHI T'CATG_A
Cuts at: 0 662 899 1587
Size: 662 237 688
Fragments arranged by size:
688 662 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 548 988 1587
Size: 218 330 440 599
Fragments arranged by size:
599 440 330 218
BsrFI r'CCGG_y
Cuts at: 0 1228 1587
Size: 1228 359
BstEII G'GTnAC_C
Cuts at: 0 1218 1587
Size: 1218 369
BstYI r'GATC_y
Cuts at: 0 1001 1587
Size: 1001 586
EaeI y'GGCC_r
Cuts at: 0 155 780 1587
Size: 155 625 807
Fragments arranged by size:
807 625 155
EagI C'GGCC_G
Cuts at: 0 155 1587
Size: 155 1432
EarI CTCTTCn'nnn_
Cuts at: 0 1095 1587
Size: 1095 492
EcoICRI GAG'CTC
Cuts at: 0 403 660 1587
Size: 403 257 927
Fragments arranged by size:
927 403 257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 436 1072 1587
Size: 289 147 636 515
Fragments arranged by size:
636 515 289 147
EcoNI CCTnn'n_nnAGG
Cuts at: 0 184 1587
Size: 184 1403
EcoO109I rG'GnC_Cy
Cuts at: 0 674 1239 1587
Size: 674 565 348
Fragments arranged by size:
674 565 348
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1044 1248 1587
Size: 1044 204 339
Fragments arranged by size:
1044 339 204
HaeII r_GCGC'y
Cuts at: 0 108 201 1587
Size: 108 93 1386
Fragments arranged by size:
1386 108 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 791 823 1072 1104 1587
Size: 136 32 623 32 249 32 483
Fragments arranged by size:
623 483 249 136 32 32 32
HincII GTy'rAC
Cuts at: 0 1466 1587
Size: 1466 121
KpnI G_GTAC'C
Cuts at: 0 1263 1334 1587
Size: 1263 71 253
Fragments arranged by size:
1263 253 71
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 500 1016 1587
Size: 122 378 516 571
Fragments arranged by size:
571 516 378 122
MscI TGG'CCA
Cuts at: 0 782 1587
Size: 782 805
MslI CAynn'nnrTG
Cuts at: 0 306 1168 1587
Size: 306 862 419
Fragments arranged by size:
862 419 306
MspA1I CmG'CkG
Cuts at: 0 1050 1511 1587
Size: 1050 461 76
Fragments arranged by size:
1050 461 76
NaeI GCC'GGC
Cuts at: 0 1230 1587
Size: 1230 357
NdeI CA'TA_TG
Cuts at: 0 636 1587
Size: 636 951
NgoMIV G'CCGG_C
Cuts at: 0 1228 1587
Size: 1228 359
PflMI CCAn_nnn'nTGG
Cuts at: 0 17 1418 1587
Size: 17 1401 169
Fragments arranged by size:
1401 169 17
PfoI T'CCnGG_A
Cuts at: 0 557 1004 1587
Size: 557 447 583
Fragments arranged by size:
583 557 447
PpuMI rG'GwC_Cy
Cuts at: 0 674 1587
Size: 674 913
PspOMI G'GGCC_C
Cuts at: 0 1238 1587
Size: 1238 349
PvuII CAG'CTG
Cuts at: 0 1050 1587
Size: 1050 537
SacI G_AGCT'C
Cuts at: 0 405 662 1587
Size: 405 257 925
Fragments arranged by size:
925 405 257
SexAI A'CCwGG_T
Cuts at: 0 801 1403 1587
Size: 801 602 184
Fragments arranged by size:
801 602 184
SfcI C'TryA_G
Cuts at: 0 293 1587
Size: 293 1294
SnaBI TAC'GTA
Cuts at: 0 1305 1587
Size: 1305 282
StuI AGG'CCT
Cuts at: 0 190 1587
Size: 190 1397
StyI C'CwwG_G
Cuts at: 0 1223 1587
Size: 1223 364
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 1587
Size: 706 881
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 775 807 1373 1405 1587
Size: 775 32 566 32 182
Fragments arranged by size:
775 566 182 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1084 1587
Size: 1084 503
XmnI GAAnn'nnTTC
Cuts at: 0 598 1027 1297 1587
Size: 598 429 270 290
Fragments arranged by size:
598 429 290 270
Enzymes that do cut:
AarI Acc65I AclI AfeI AflIII AloI AlwNI ApaI
BanI BanII BbsI BbvCI BciVI Bme1580I BmrI BpmI
Bpu10I BsaAI BsaBI BsaXI BseRI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrFI BstEII BstYI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KpnI MmeI MscI MslI MspA1I NaeI NdeI
NgoMIV PflMI PfoI PpuMI PspOMI PvuII SacI SexAI
SfcI SnaBI StuI StyI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AatII AccI AcuI AflII AgeI AhdI AleI AlfI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpuEI BsaI BsaHI BsaWI BseYI
BsgI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI
MluI NarI NcoI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI SwaI TatI XbaI
XcmI XhoI XmaI ZraI