Restriction map for
pFN21AE4503
(Linear) (Six-base) MAPSORT of: KIEE4503.seq Check: 8261 from: 1 to: 1584
KIEE4503.orf
With 182 enzymes: SgfI *
March 22, 2012 18:13 ..
AccI GT'mk_AC
Cuts at: 0 372 863 1584
Size: 372 491 721
Fragments arranged by size:
721 491 372
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 1272 1315 1565 1584
Size: 177 1095 43 250 19
Fragments arranged by size:
1095 250 177 43 19
AflIII A'CryG_T
Cuts at: 0 136 1584
Size: 136 1448
ApaI G_GGCC'C
Cuts at: 0 689 1025 1408 1529 1584
Size: 689 336 383 121 55
Fragments arranged by size:
689 383 336 121 55
AvaI C'yCGr_G
Cuts at: 0 11 420 441 1184 1584
Size: 11 409 21 743 400
Fragments arranged by size:
743 409 400 21 11
BamHI G'GATC_C
Cuts at: 0 280 1584
Size: 280 1304
BanI G'GyrC_C
Cuts at: 0 15 106 784 1276 1441 1584
Size: 15 91 678 492 165 143
Fragments arranged by size:
678 492 165 143 91 15
BanII G_rGCy'C
Cuts at: 0 7 689 1025 1156 1261 1408 1529 1584
Size: 7 682 336 131 105 147 121 55
Fragments arranged by size:
682 336 147 131 121 105 55 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1070 1104 1584
Size: 1070 34 480
Fragments arranged by size:
1070 480 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 618 1584
Size: 618 966
BglI GCCn_nnn'nGGC
Cuts at: 0 833 1412 1524 1584
Size: 833 579 112 60
Fragments arranged by size:
833 579 112 60
Bme1580I G_kGCm'C
Cuts at: 0 18 44 689 789 1025 1408 1529 1584
Size: 18 26 645 100 236 383 121 55
Fragments arranged by size:
645 383 236 121 100 55 26 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 179 1409 1584
Size: 179 1230 175
Fragments arranged by size:
1230 179 175
BmtI G_CTAG'C
Cuts at: 0 1537 1584
Size: 1537 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 195 246 258 812 980 1035 1161
Size: 168 27 51 12 554 168 55 126
Cuts at: 1161 1284 1443 1584
Size: 123 159 141
Fragments arranged by size:
554 168 168 159 141 126 123 55
51 27 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 76 1584
Size: 76 1508
BsaWI w'CCGG_w
Cuts at: 0 355 385 1219 1584
Size: 355 30 834 365
Fragments arranged by size:
834 365 355 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 1584
Size: 193 30 1361
Fragments arranged by size:
1361 193 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 342 891 1374 1584
Size: 64 278 549 483 210
Fragments arranged by size:
549 483 278 210 64
BseYI C'CCAG_C
Cuts at: 0 395 399 566 718 1018 1069 1192 1529
Size: 395 4 167 152 300 51 123 337
Cuts at: 1529 1584
Size: 55
Fragments arranged by size:
395 337 300 167 152 123 55 51
4
BsmI GAATG_Cn'
Cuts at: 0 1099 1584
Size: 1099 485
BsmBI CGTCTCn'nnnn_
Cuts at: 0 14 699 1584
Size: 14 685 885
Fragments arranged by size:
885 685 14
Bsp1286I G_dGCh'C
Cuts at: 0 7 18 44 689 789 1025 1156 1261
Size: 7 11 26 645 100 236 131 105
Cuts at: 1261 1408 1529 1584
Size: 147 121 55
Fragments arranged by size:
645 236 147 131 121 105 100 55
26 11 7
BspEI T'CCGG_A
Cuts at: 0 355 1584
Size: 355 1229
BsrBI CCG'CTC
Cuts at: 0 1487 1584
Size: 1487 97
BsrDI GCAATG_nn'
Cuts at: 0 287 825 1094 1584
Size: 287 538 269 490
Fragments arranged by size:
538 490 287 269
BsrFI r'CCGG_y
Cuts at: 0 1279 1584
Size: 1279 305
BstEII G'GTnAC_C
Cuts at: 0 1363 1584
Size: 1363 221
BstXI CCAn_nnnn'nTGG
Cuts at: 0 601 1135 1315 1584
Size: 601 534 180 269
Fragments arranged by size:
601 534 269 180
BstYI r'GATC_y
Cuts at: 0 280 310 358 1475 1584
Size: 280 30 48 1117 109
Fragments arranged by size:
1117 280 109 48 30
BtgI C'CryG_G
Cuts at: 0 546 1148 1584
Size: 546 602 436
Fragments arranged by size:
602 546 436
BtsI GCAGTG_nn'
Cuts at: 0 55 621 684 1251 1470 1584
Size: 55 566 63 567 219 114
Fragments arranged by size:
567 566 219 114 63 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1434 1469 1584
Size: 1434 35 115
Fragments arranged by size:
1434 115 35
DraIII CAC_nnn'GTG
Cuts at: 0 732 1584
Size: 732 852
EaeI y'GGCC_r
Cuts at: 0 618 1091 1584
Size: 618 473 493
Fragments arranged by size:
618 493 473
EarI CTCTTCn'nnn_
Cuts at: 0 588 594 1362 1584
Size: 588 6 768 222
Fragments arranged by size:
768 588 222 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 177 195 246 258 812 980 1035
Size: 168 9 18 51 12 554 168 55
Cuts at: 1035 1161 1272 1284 1315 1443 1565 1584
Size: 126 111 12 31 128 122 19
Fragments arranged by size:
554 168 168 128 126 122 111 55
51 31 19 18 12 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 986 1584
Size: 986 598
EcoO109I rG'GnC_Cy
Cuts at: 0 86 129 685 805 1002 1022 1162 1404
Size: 86 43 556 120 197 20 140 242
Cuts at: 1404 1405 1525 1526 1584
Size: 1 120 1 58
Fragments arranged by size:
556 242 197 140 120 120 86 58
43 20 1 1
EcoRV GAT'ATC
Cuts at: 0 1500 1584
Size: 1500 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 674 706 1584
Size: 674 32 878
Fragments arranged by size:
878 674 32
MluI A'CGCG_T
Cuts at: 0 136 1584
Size: 136 1448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1584
Size: 659 925
MscI TGG'CCA
Cuts at: 0 620 1584
Size: 620 964
MspA1I CmG'CkG
Cuts at: 0 188 278 399 431 548 1018 1438 1584
Size: 188 90 121 32 117 470 420 146
Fragments arranged by size:
470 420 188 146 121 117 90 32
NheI G'CTAG_C
Cuts at: 0 1533 1584
Size: 1533 51
NspI r_CATG'y
Cuts at: 0 1377 1584
Size: 1377 207
PasI CC'CwG_GG
Cuts at: 0 294 1584
Size: 294 1290
PflMI CCAn_nnn'nTGG
Cuts at: 0 1110 1584
Size: 1110 474
PfoI T'CCnGG_A
Cuts at: 0 1493 1584
Size: 1493 91
PpuMI rG'GwC_Cy
Cuts at: 0 86 129 805 1002 1162 1584
Size: 86 43 676 197 160 422
Fragments arranged by size:
676 422 197 160 86 43
PspOMI G'GGCC_C
Cuts at: 0 685 1021 1404 1525 1584
Size: 685 336 383 121 59
Fragments arranged by size:
685 383 336 121 59
PstI C_TGCA'G
Cuts at: 0 309 679 1584
Size: 309 370 905
Fragments arranged by size:
905 370 309
PvuII CAG'CTG
Cuts at: 0 399 1018 1584
Size: 399 619 566
Fragments arranged by size:
619 566 399
SacII CC_GC'GG
Cuts at: 0 549 1584
Size: 549 1035
SanDI GG'GwC_CC
Cuts at: 0 1002 1162 1584
Size: 1002 160 422
Fragments arranged by size:
1002 422 160
SapI GCTCTTCn'nnn_
Cuts at: 0 588 1362 1584
Size: 588 774 222
Fragments arranged by size:
774 588 222
SfcI C'TryA_G
Cuts at: 0 201 305 648 675 813 860 933 1584
Size: 201 104 343 27 138 47 73 651
Fragments arranged by size:
651 343 201 138 104 73 47 27
SmlI C'TyrA_G
Cuts at: 0 1184 1584
Size: 1184 400
StuI AGG'CCT
Cuts at: 0 750 1584
Size: 750 834
StyI C'CwwG_G
Cuts at: 0 89 1157 1560 1584
Size: 89 1068 403 24
Fragments arranged by size:
1068 403 89 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 883 1584
Size: 25 858 701
Fragments arranged by size:
858 701 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 15 47 1584
Size: 15 32 1537
Fragments arranged by size:
1537 32 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 508 778 1584
Size: 508 270 806
Fragments arranged by size:
806 508 270
XhoI C'TCGA_G
Cuts at: 0 1184 1584
Size: 1184 400
Enzymes that do cut:
AccI AcuI AflIII ApaI AvaI BamHI BanI BanII
BcgI BciVI BglI Bme1580I BmrI BmtI BpmI BsaI
BsaWI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I BspEI
BsrBI BsrDI BsrFI BstEII BstXI BstYI BtgI BtsI
CspCI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRV
Hin4I MluI MmeI MscI MspA1I NheI NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacII SanDI
SapI SfcI SmlI StuI StyI TaqII TstI XcmI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BclI BfrBI
BglII BlpI BmgBI BplI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsgI BsiEI BsiHKAI BsiWI BspHI BspMI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI
DraI DrdI EagI EciI EcoICRI EcoRI FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MslI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XmaI XmnI ZraI