Restriction map for
pFN21AE4495
(Linear) (Six-base) MAPSORT of: KIEE4495.seq Check: 8753 from: 1 to: 1557
KIEE4495.orf
With 182 enzymes: SgfI *
March 22, 2012 18:12 ..
Acc65I G'GTAC_C
Cuts at: 0 662 1557
Size: 662 895
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1017 1557
Size: 1017 540
AgeI A'CCGG_T
Cuts at: 0 340 659 1557
Size: 340 319 898
Fragments arranged by size:
898 340 319
AleI CACnn'nnGTG
Cuts at: 0 187 1557
Size: 187 1370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 67 205 970 1557
Size: 67 138 765 587
Fragments arranged by size:
765 587 138 67
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 376 408 1557
Size: 376 32 1149
Fragments arranged by size:
1149 376 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1260 1557
Size: 1260 297
ApaI G_GGCC'C
Cuts at: 0 506 1557
Size: 506 1051
ApaLI G'TGCA_C
Cuts at: 0 180 288 1557
Size: 180 108 1269
Fragments arranged by size:
1269 180 108
ApoI r'AATT_y
Cuts at: 0 265 1557
Size: 265 1292
AvaI C'yCGr_G
Cuts at: 0 77 1557
Size: 77 1480
BanI G'GyrC_C
Cuts at: 0 427 656 662 1557
Size: 427 229 6 895
Fragments arranged by size:
895 427 229 6
BanII G_rGCy'C
Cuts at: 0 7 63 506 1557
Size: 7 56 443 1051
Fragments arranged by size:
1051 443 56 7
BbeI G_GCGC'C
Cuts at: 0 431 1557
Size: 431 1126
BbsI GAAGACnn'nnnn_
Cuts at: 0 1494 1557
Size: 1494 63
BclI T'GATC_A
Cuts at: 0 470 1557
Size: 470 1087
BglII A'GATC_T
Cuts at: 0 435 687 1557
Size: 435 252 870
Fragments arranged by size:
870 435 252
Bme1580I G_kGCm'C
Cuts at: 0 184 292 506 1557
Size: 184 108 214 1051
Fragments arranged by size:
1051 214 184 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 755 1557
Size: 755 802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 676 822 976 1295 1382 1557
Size: 231 445 146 154 319 87 175
Fragments arranged by size:
445 319 231 175 154 146 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 813 1073 1557
Size: 417 396 260 484
Fragments arranged by size:
484 417 396 260
BsaI GGTCTCn'nnnn_
Cuts at: 0 789 1557
Size: 789 768
BsaAI yAC'GTr
Cuts at: 0 468 1557
Size: 468 1089
BsaHI Gr'CG_yC
Cuts at: 0 428 603 1557
Size: 428 175 954
Fragments arranged by size:
954 428 175
BsaWI w'CCGG_w
Cuts at: 0 275 340 659 1557
Size: 275 65 319 898
Fragments arranged by size:
898 319 275 65
BseYI C'CCAG_C
Cuts at: 0 489 515 842 1557
Size: 489 26 327 715
Fragments arranged by size:
715 489 327 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 1557
Size: 155 1402
BsiHKAI G_wGCw'C
Cuts at: 0 184 292 1557
Size: 184 108 1265
Fragments arranged by size:
1265 184 108
BsmI GAATG_Cn'
Cuts at: 0 1050 1557
Size: 1050 507
Bsp1286I G_dGCh'C
Cuts at: 0 7 63 184 292 506 1557
Size: 7 56 121 108 214 1051
Fragments arranged by size:
1051 214 121 108 56 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 289 648 1557
Size: 289 359 909
Fragments arranged by size:
909 359 289
BsrBI CCG'CTC
Cuts at: 0 357 1557
Size: 357 1200
BsrDI GCAATG_nn'
Cuts at: 0 714 903 1557
Size: 714 189 654
Fragments arranged by size:
714 654 189
BsrFI r'CCGG_y
Cuts at: 0 304 340 659 1557
Size: 304 36 319 898
Fragments arranged by size:
898 319 304 36
BsrGI T'GTAC_A
Cuts at: 0 769 1286 1557
Size: 769 517 271
Fragments arranged by size:
769 517 271
BstBI TT'CG_AA
Cuts at: 0 1494 1557
Size: 1494 63
BstEII G'GTnAC_C
Cuts at: 0 885 1557
Size: 885 672
BstXI CCAn_nnnn'nTGG
Cuts at: 0 784 1176 1557
Size: 784 392 381
Fragments arranged by size:
784 392 381
BstYI r'GATC_y
Cuts at: 0 435 687 1557
Size: 435 252 870
Fragments arranged by size:
870 435 252
Bsu36I CC'TnA_GG
Cuts at: 0 404 1253 1557
Size: 404 849 304
Fragments arranged by size:
849 404 304
BtsI GCAGTG_nn'
Cuts at: 0 184 543 1557
Size: 184 359 1014
Fragments arranged by size:
1014 359 184
ClaI AT'CG_AT
Cuts at: 0 1370 1557
Size: 1370 187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 135 170 1557
Size: 135 35 1387
Fragments arranged by size:
1387 135 35
DraIII CAC_nnn'GTG
Cuts at: 0 893 1557
Size: 893 664
EaeI y'GGCC_r
Cuts at: 0 143 256 1557
Size: 143 113 1301
Fragments arranged by size:
1301 143 113
EarI CTCTTCn'nnn_
Cuts at: 0 442 1557
Size: 442 1115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 676 822 976 1017 1295 1382 1557
Size: 231 445 146 154 41 278 87 175
Fragments arranged by size:
445 278 231 175 154 146 87 41
EcoO109I rG'GnC_Cy
Cuts at: 0 206 637 1557
Size: 206 431 920
Fragments arranged by size:
920 431 206
HaeII r_GCGC'y
Cuts at: 0 431 1557
Size: 431 1126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 912 944 970 1002 1557
Size: 912 32 26 32 555
Fragments arranged by size:
912 555 32 32 26
KasI G'GCGC_C
Cuts at: 0 427 1557
Size: 427 1130
KpnI G_GTAC'C
Cuts at: 0 666 1557
Size: 666 891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 832 1557
Size: 802 30 725
Fragments arranged by size:
802 725 30
MscI TGG'CCA
Cuts at: 0 258 1557
Size: 258 1299
MslI CAynn'nnrTG
Cuts at: 0 91 187 1174 1557
Size: 91 96 987 383
Fragments arranged by size:
987 383 96 91
MspA1I CmG'CkG
Cuts at: 0 780 842 1557
Size: 780 62 715
Fragments arranged by size:
780 715 62
NaeI GCC'GGC
Cuts at: 0 306 1557
Size: 306 1251
NarI GG'CG_CC
Cuts at: 0 428 1557
Size: 428 1129
NgoMIV G'CCGG_C
Cuts at: 0 304 1557
Size: 304 1253
NruI TCG'CGA
Cuts at: 0 442 1557
Size: 442 1115
PasI CC'CwG_GG
Cuts at: 0 63 1557
Size: 63 1494
PpuMI rG'GwC_Cy
Cuts at: 0 637 1557
Size: 637 920
PspOMI G'GGCC_C
Cuts at: 0 502 1557
Size: 502 1055
PstI C_TGCA'G
Cuts at: 0 538 1242 1557
Size: 538 704 315
Fragments arranged by size:
704 538 315
PvuII CAG'CTG
Cuts at: 0 780 842 1557
Size: 780 62 715
Fragments arranged by size:
780 715 62
SexAI A'CCwGG_T
Cuts at: 0 881 1557
Size: 881 676
SfcI C'TryA_G
Cuts at: 0 534 609 1238 1356 1557
Size: 534 75 629 118 201
Fragments arranged by size:
629 534 201 118 75
SfoI GGC'GCC
Cuts at: 0 429 1557
Size: 429 1128
SmlI C'TyrA_G
Cuts at: 0 396 828 1088 1557
Size: 396 432 260 469
Fragments arranged by size:
469 432 396 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 121 1557
Size: 121 1436
TatI w'GTAC_w
Cuts at: 0 453 769 1286 1557
Size: 453 316 517 271
Fragments arranged by size:
517 453 316 271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 744 776 1424 1456 1557
Size: 744 32 648 32 101
Fragments arranged by size:
744 648 101 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 700 1557
Size: 700 857
Enzymes that do cut:
Acc65I AcuI AgeI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BclI
BglII Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI Bsu36I
BtsI ClaI CspCI DraIII EaeI EarI Eco57MI EcoO109I
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI PasI PpuMI PspOMI PstI
PvuII SexAI SfcI SfoI SmlI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I
BsaBI BsaXI BseRI BsiEI BsiWI BsmBI BspEI BspHI
BssHII BssSI BstAPI BstZ17I BtgI BtgZI DraI DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI NcoI
NdeI NheI NotI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI