Restriction map for
pFN21AE4494
(Linear) (Six-base) MAPSORT of: KIEE4494.seq Check: 9507 from: 1 to: 1545
KIEE4494.orf
With 182 enzymes: SgfI *
March 22, 2012 18:12 ..
AatII G_ACGT'C
Cuts at: 0 851 1331 1545
Size: 851 480 214
Fragments arranged by size:
851 480 214
AccI GT'mk_AC
Cuts at: 0 437 1545
Size: 437 1108
Acc65I G'GTAC_C
Cuts at: 0 962 1545
Size: 962 583
AhdI GACnn_n'nnGTC
Cuts at: 0 546 1545
Size: 546 999
AlwNI CAG_nnn'CTG
Cuts at: 0 232 801 1545
Size: 232 569 744
Fragments arranged by size:
744 569 232
ApoI r'AATT_y
Cuts at: 0 754 1545
Size: 754 791
AvaI C'yCGr_G
Cuts at: 0 1243 1545
Size: 1243 302
BamHI G'GATC_C
Cuts at: 0 41 1545
Size: 41 1504
BanI G'GyrC_C
Cuts at: 0 962 981 1053 1513 1545
Size: 962 19 72 460 32
Fragments arranged by size:
962 460 72 32 19
BanII G_rGCy'C
Cuts at: 0 257 538 719 1373 1545
Size: 257 281 181 654 172
Fragments arranged by size:
654 281 257 181 172
BbsI GAAGACnn'nnnn_
Cuts at: 0 1323 1545
Size: 1323 222
BciVI GTATCCnnnnn_n'
Cuts at: 0 81 1545
Size: 81 1464
BfrBI ATG'CAT
Cuts at: 0 881 1545
Size: 881 664
BglI GCCn_nnn'nGGC
Cuts at: 0 485 1545
Size: 485 1060
BglII A'GATC_T
Cuts at: 0 140 584 1545
Size: 140 444 961
Fragments arranged by size:
961 444 140
Bme1580I G_kGCm'C
Cuts at: 0 986 1058 1516 1545
Size: 986 72 458 29
Fragments arranged by size:
986 458 72 29
BmgBI CAC'GTC
Cuts at: 0 774 1545
Size: 774 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1441 1545
Size: 313 1128 104
Fragments arranged by size:
1128 313 104
Bpu10I CC'TnA_GC
Cuts at: 0 19 1545
Size: 19 1526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 1545
Size: 271 1274
BsaAI yAC'GTr
Cuts at: 0 924 1545
Size: 924 621
BsaHI Gr'CG_yC
Cuts at: 0 848 1304 1328 1545
Size: 848 456 24 217
Fragments arranged by size:
848 456 217 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 547 574 577 1545
Size: 544 3 27 3 968
Fragments arranged by size:
968 544 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 552 1261 1545
Size: 292 260 709 284
Fragments arranged by size:
709 292 284 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 913 1201 1390 1545
Size: 643 270 288 189 155
Fragments arranged by size:
643 288 270 189 155
BsiEI CG_ry'CG
Cuts at: 0 434 1545
Size: 434 1111
BsiHKAI G_wGCw'C
Cuts at: 0 538 1373 1545
Size: 538 835 172
Fragments arranged by size:
835 538 172
BsmI GAATG_Cn'
Cuts at: 0 381 1545
Size: 381 1164
Bsp1286I G_dGCh'C
Cuts at: 0 257 538 719 986 1058 1373 1516 1545
Size: 257 281 181 267 72 315 143 29
Fragments arranged by size:
315 281 267 257 181 143 72 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 1545
Size: 536 1009
BsrFI r'CCGG_y
Cuts at: 0 542 1129 1545
Size: 542 587 416
Fragments arranged by size:
587 542 416
BsrGI T'GTAC_A
Cuts at: 0 1199 1545
Size: 1199 346
BssSI C'ACGA_G
Cuts at: 0 463 1545
Size: 463 1082
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1356 1545
Size: 1356 189
BstEII G'GTnAC_C
Cuts at: 0 121 1545
Size: 121 1424
BstXI CCAn_nnnn'nTGG
Cuts at: 0 906 1545
Size: 906 639
BstYI r'GATC_y
Cuts at: 0 41 140 584 656 1545
Size: 41 99 444 72 889
Fragments arranged by size:
889 444 99 72 41
BtgI C'CryG_G
Cuts at: 0 815 977 987 1466 1545
Size: 815 162 10 479 79
Fragments arranged by size:
815 479 162 79 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 83 672 1545
Size: 83 589 873
Fragments arranged by size:
873 589 83
BtsI GCAGTG_nn'
Cuts at: 0 1156 1399 1545
Size: 1156 243 146
Fragments arranged by size:
1156 243 146
DraIII CAC_nnn'GTG
Cuts at: 0 1356 1545
Size: 1356 189
EaeI y'GGCC_r
Cuts at: 0 128 327 812 1127 1545
Size: 128 199 485 315 418
Fragments arranged by size:
485 418 315 199 128
EarI CTCTTCn'nnn_
Cuts at: 0 47 415 1483 1545
Size: 47 368 1068 62
Fragments arranged by size:
1068 368 62 47
EcoICRI GAG'CTC
Cuts at: 0 536 1371 1545
Size: 536 835 174
Fragments arranged by size:
835 536 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1441 1545
Size: 313 1128 104
Fragments arranged by size:
1128 313 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 519 610 1545
Size: 519 91 935
Fragments arranged by size:
935 519 91
EcoO109I rG'GnC_Cy
Cuts at: 0 525 1080 1462 1545
Size: 525 555 382 83
Fragments arranged by size:
555 525 382 83
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 1545
Size: 735 810
FspI TGC'GCA
Cuts at: 0 1360 1545
Size: 1360 185
HaeII r_GCGC'y
Cuts at: 0 360 1317 1545
Size: 360 957 228
Fragments arranged by size:
957 360 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 532 564 789 821 1545
Size: 331 32 169 32 225 32 724
Fragments arranged by size:
724 331 225 169 32 32 32
KpnI G_GTAC'C
Cuts at: 0 966 1545
Size: 966 579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 575 1232 1545
Size: 65 510 657 313
Fragments arranged by size:
657 510 313 65
MscI TGG'CCA
Cuts at: 0 130 329 814 1545
Size: 130 199 485 731
Fragments arranged by size:
731 485 199 130
MslI CAynn'nnrTG
Cuts at: 0 106 374 502 1207 1545
Size: 106 268 128 705 338
Fragments arranged by size:
705 338 268 128 106
MspA1I CmG'CkG
Cuts at: 0 989 1545
Size: 989 556
NaeI GCC'GGC
Cuts at: 0 1131 1545
Size: 1131 414
NcoI C'CATG_G
Cuts at: 0 815 1545
Size: 815 730
NgoMIV G'CCGG_C
Cuts at: 0 1129 1545
Size: 1129 416
NsiI A_TGCA'T
Cuts at: 0 883 1545
Size: 883 662
NspI r_CATG'y
Cuts at: 0 1233 1545
Size: 1233 312
PflMI CCAn_nnn'nTGG
Cuts at: 0 155 158 606 924 1125 1545
Size: 155 3 448 318 201 420
Fragments arranged by size:
448 420 318 201 155 3
PfoI T'CCnGG_A
Cuts at: 0 167 767 1322 1545
Size: 167 600 555 223
Fragments arranged by size:
600 555 223 167
PpuMI rG'GwC_Cy
Cuts at: 0 525 1545
Size: 525 1020
PstI C_TGCA'G
Cuts at: 0 533 797 1115 1545
Size: 533 264 318 430
Fragments arranged by size:
533 430 318 264
SacI G_AGCT'C
Cuts at: 0 538 1373 1545
Size: 538 835 172
Fragments arranged by size:
835 538 172
SacII CC_GC'GG
Cuts at: 0 990 1545
Size: 990 555
SapI GCTCTTCn'nnn_
Cuts at: 0 47 1545
Size: 47 1498
SfcI C'TryA_G
Cuts at: 0 529 793 1111 1545
Size: 529 264 318 434
Fragments arranged by size:
529 434 318 264
SmlI C'TyrA_G
Cuts at: 0 286 1545
Size: 286 1259
StuI AGG'CCT
Cuts at: 0 1381 1545
Size: 1381 164
StyI C'CwwG_G
Cuts at: 0 815 1545
Size: 815 730
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 1515 1545
Size: 100 1415 30
Fragments arranged by size:
1415 100 30
TatI w'GTAC_w
Cuts at: 0 1199 1545
Size: 1199 346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 547 579 1545
Size: 547 32 966
Fragments arranged by size:
966 547 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 963 1545
Size: 963 582
XmnI GAAnn'nnTTC
Cuts at: 0 514 1545
Size: 514 1031
ZraI GAC'GTC
Cuts at: 0 849 1329 1545
Size: 849 480 216
Fragments arranged by size:
849 480 216
Enzymes that do cut:
AatII AccI Acc65I AhdI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BciVI BfrBI BglI BglII Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PflMI PfoI PpuMI
PstI SacI SacII SapI SfcI SmlI StuI StyI
TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AcuI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BclI BlpI BmrI BmtI
BplI BsaI BsaBI BsaWI BseYI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BssHII BstBI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KasI MfeI MluI NarI
NdeI NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI