Restriction map for
pFN21AE4493
(Linear) (Six-base) MAPSORT of: KIEE4493.seq Check: 6927 from: 1 to: 1539
KIEE4493.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 1170 1539
Size: 562 608 369
Fragments arranged by size:
608 562 369
AlwNI CAG_nnn'CTG
Cuts at: 0 715 1339 1539
Size: 715 624 200
Fragments arranged by size:
715 624 200
ApaLI G'TGCA_C
Cuts at: 0 75 1539
Size: 75 1464
AvaI C'yCGr_G
Cuts at: 0 1264 1539
Size: 1264 275
BamHI G'GATC_C
Cuts at: 0 482 1539
Size: 482 1057
BanI G'GyrC_C
Cuts at: 0 94 171 566 646 929 949 1539
Size: 94 77 395 80 283 20 590
Fragments arranged by size:
590 395 283 94 80 77 20
BanII G_rGCy'C
Cuts at: 0 784 1088 1539
Size: 784 304 451
Fragments arranged by size:
784 451 304
BbeI G_GCGC'C
Cuts at: 0 570 933 1539
Size: 570 363 606
Fragments arranged by size:
606 570 363
BbsI GAAGACnn'nnnn_
Cuts at: 0 746 1539
Size: 746 793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 813 847 1033 1067 1539
Size: 813 34 186 34 472
Fragments arranged by size:
813 472 186 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 92 126 1539
Size: 92 34 1413
Fragments arranged by size:
1413 92 34
BglII A'GATC_T
Cuts at: 0 818 1539
Size: 818 721
Bme1580I G_kGCm'C
Cuts at: 0 79 97 1121 1539
Size: 79 18 1024 418
Fragments arranged by size:
1024 418 79 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 960 1100 1539
Size: 960 140 439
Fragments arranged by size:
960 439 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 247 1027 1072 1539
Size: 73 174 780 45 467
Fragments arranged by size:
780 467 174 73 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1539
Size: 622 917
BsaHI Gr'CG_yC
Cuts at: 0 332 567 930 1037 1539
Size: 332 235 363 107 502
Fragments arranged by size:
502 363 332 235 107
BseYI C'CCAG_C
Cuts at: 0 251 1327 1539
Size: 251 1076 212
Fragments arranged by size:
1076 251 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1539
Size: 258 1281
BsiEI CG_ry'CG
Cuts at: 0 44 321 1539
Size: 44 277 1218
Fragments arranged by size:
1218 277 44
BsiHKAI G_wGCw'C
Cuts at: 0 79 946 1088 1508 1539
Size: 79 867 142 420 31
Fragments arranged by size:
867 420 142 79 31
BsmI GAATG_Cn'
Cuts at: 0 887 1539
Size: 887 652
Bsp1286I G_dGCh'C
Cuts at: 0 79 97 784 946 1088 1121 1508 1539
Size: 79 18 687 162 142 33 387 31
Fragments arranged by size:
687 387 162 142 79 33 31 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1139 1539
Size: 1139 400
BsrBI CCG'CTC
Cuts at: 0 440 1539
Size: 440 1099
BsrDI GCAATG_nn'
Cuts at: 0 987 1539
Size: 987 552
BsrFI r'CCGG_y
Cuts at: 0 43 1539
Size: 43 1496
BssHII G'CGCG_C
Cuts at: 0 264 1539
Size: 264 1275
BssSI C'ACGA_G
Cuts at: 0 423 1068 1539
Size: 423 645 471
Fragments arranged by size:
645 471 423
BstAPI GCAn_nnn'nTGC
Cuts at: 0 986 1539
Size: 986 553
BstXI CCAn_nnnn'nTGG
Cuts at: 0 469 765 1539
Size: 469 296 774
Fragments arranged by size:
774 469 296
BstYI r'GATC_y
Cuts at: 0 482 818 1539
Size: 482 336 721
Fragments arranged by size:
721 482 336
Bsu36I CC'TnA_GG
Cuts at: 0 1306 1539
Size: 1306 233
BtgI C'CryG_G
Cuts at: 0 134 321 809 920 1437 1539
Size: 134 187 488 111 517 102
Fragments arranged by size:
517 488 187 134 111 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 321 1539
Size: 321 1218
BtsI GCAGTG_nn'
Cuts at: 0 1119 1194 1264 1364 1539
Size: 1119 75 70 100 175
Fragments arranged by size:
1119 175 100 75 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 602 637 1539
Size: 602 35 902
Fragments arranged by size:
902 602 35
DraIII CAC_nnn'GTG
Cuts at: 0 1125 1539
Size: 1125 414
DrdI GACnn_nn'nnGTC
Cuts at: 0 589 1539
Size: 589 950
EaeI y'GGCC_r
Cuts at: 0 41 698 1539
Size: 41 657 841
Fragments arranged by size:
841 657 41
EagI C'GGCC_G
Cuts at: 0 41 1539
Size: 41 1498
EarI CTCTTCn'nnn_
Cuts at: 0 875 1244 1539
Size: 875 369 295
Fragments arranged by size:
875 369 295
EcoICRI GAG'CTC
Cuts at: 0 1086 1539
Size: 1086 453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 247 562 1027 1072 1170 1539
Size: 73 174 315 465 45 98 369
Fragments arranged by size:
465 369 315 174 98 73 45
EcoO109I rG'GnC_Cy
Cuts at: 0 913 1539
Size: 913 626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1257 1539
Size: 1257 282
HaeII r_GCGC'y
Cuts at: 0 301 570 933 1539
Size: 301 269 363 606
Fragments arranged by size:
606 363 301 269
KasI G'GCGC_C
Cuts at: 0 566 929 1539
Size: 566 363 610
Fragments arranged by size:
610 566 363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1067 1539
Size: 782 285 472
Fragments arranged by size:
782 472 285
MslI CAynn'nnrTG
Cuts at: 0 1381 1539
Size: 1381 158
MspA1I CmG'CkG
Cuts at: 0 136 323 453 663 974 1539
Size: 136 187 130 210 311 565
Fragments arranged by size:
565 311 210 187 136 130
NaeI GCC'GGC
Cuts at: 0 45 1539
Size: 45 1494
NarI GG'CG_CC
Cuts at: 0 567 930 1539
Size: 567 363 609
Fragments arranged by size:
609 567 363
NcoI C'CATG_G
Cuts at: 0 920 1539
Size: 920 619
NgoMIV G'CCGG_C
Cuts at: 0 43 1539
Size: 43 1496
PasI CC'CwG_GG
Cuts at: 0 124 398 462 1539
Size: 124 274 64 1077
Fragments arranged by size:
1077 274 124 64
PflMI CCAn_nnn'nTGG
Cuts at: 0 494 1539
Size: 494 1045
PfoI T'CCnGG_A
Cuts at: 0 224 1004 1539
Size: 224 780 535
Fragments arranged by size:
780 535 224
PspXI vC'TCGA_Gb
Cuts at: 0 1264 1539
Size: 1264 275
PstI C_TGCA'G
Cuts at: 0 314 979 1539
Size: 314 665 560
Fragments arranged by size:
665 560 314
PvuII CAG'CTG
Cuts at: 0 453 663 1539
Size: 453 210 876
Fragments arranged by size:
876 453 210
SacI G_AGCT'C
Cuts at: 0 1088 1539
Size: 1088 451
SacII CC_GC'GG
Cuts at: 0 137 324 1539
Size: 137 187 1215
Fragments arranged by size:
1215 187 137
SapI GCTCTTCn'nnn_
Cuts at: 0 1244 1539
Size: 1244 295
SexAI A'CCwGG_T
Cuts at: 0 617 1508 1539
Size: 617 891 31
Fragments arranged by size:
891 617 31
SfcI C'TryA_G
Cuts at: 0 310 975 1539
Size: 310 665 564
Fragments arranged by size:
665 564 310
SfoI GGC'GCC
Cuts at: 0 568 931 1539
Size: 568 363 608
Fragments arranged by size:
608 568 363
SmlI C'TyrA_G
Cuts at: 0 637 1264 1539
Size: 637 627 275
Fragments arranged by size:
637 627 275
StuI AGG'CCT
Cuts at: 0 493 1539
Size: 493 1046
StyI C'CwwG_G
Cuts at: 0 488 920 1539
Size: 488 432 619
Fragments arranged by size:
619 488 432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 192 295 701 979 1469 1476 1539
Size: 112 80 103 406 278 490 7 63
Fragments arranged by size:
490 406 278 112 103 80 63 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 868 900 1539
Size: 868 32 639
Fragments arranged by size:
868 639 32
XhoI C'TCGA_G
Cuts at: 0 1264 1539
Size: 1264 275
Enzymes that do cut:
AcuI AlwNI ApaLI AvaI BamHI BanI BanII BbeI
BbsI BcgI BglI BglII Bme1580I BmrI BpmI BpuEI
BsaHI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII KasI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PflMI PfoI PspXI PstI PvuII SacI SacII SapI
SexAI SfcI SfoI SmlI StuI StyI TaqII TstI
XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsiWI BsmBI BspEI BspHI BsrGI
BstBI BstEII BstZ17I ClaI DraI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MscI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI
XmaI XmnI ZraI