Restriction map for
pFN21AE4492
(Linear) (Six-base) MAPSORT of: KIEE4492.seq Check: 3191 from: 1 to: 1536
KIEE4492.orf
With 182 enzymes: SgfI *
March 22, 2012 18:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 101 1536
Size: 101 1435
AccI GT'mk_AC
Cuts at: 0 478 1536
Size: 478 1058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 944 1528 1536
Size: 469 475 584 8
Fragments arranged by size:
584 475 469 8
AflIII A'CryG_T
Cuts at: 0 481 1536
Size: 481 1055
AleI CACnn'nnGTG
Cuts at: 0 1180 1536
Size: 1180 356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1247 1391 1536
Size: 1247 144 145
Fragments arranged by size:
1247 145 144
AlwNI CAG_nnn'CTG
Cuts at: 0 1358 1536
Size: 1358 178
ApaI G_GGCC'C
Cuts at: 0 222 395 1352 1522 1536
Size: 222 173 957 170 14
Fragments arranged by size:
957 222 173 170 14
ApaLI G'TGCA_C
Cuts at: 0 582 1536
Size: 582 954
ApoI r'AATT_y
Cuts at: 0 133 1536
Size: 133 1403
AvaI C'yCGr_G
Cuts at: 0 175 515 878 1536
Size: 175 340 363 658
Fragments arranged by size:
658 363 340 175
AvrII C'CTAG_G
Cuts at: 0 1047 1536
Size: 1047 489
BanI G'GyrC_C
Cuts at: 0 3 845 874 1536
Size: 3 842 29 662
Fragments arranged by size:
842 662 29 3
BanII G_rGCy'C
Cuts at: 0 222 395 1352 1522 1536
Size: 222 173 957 170 14
Fragments arranged by size:
957 222 173 170 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1000 1034 1536
Size: 1000 34 502
Fragments arranged by size:
1000 502 34
BfrBI ATG'CAT
Cuts at: 0 1224 1306 1536
Size: 1224 82 230
Fragments arranged by size:
1224 230 82
BlpI GC'TnA_GC
Cuts at: 0 1162 1536
Size: 1162 374
Bme1580I G_kGCm'C
Cuts at: 0 8 222 395 586 879 1145 1352 1522
Size: 8 214 173 191 293 266 207 170
Cuts at: 1522 1536
Size: 14
Fragments arranged by size:
293 266 214 207 191 173 170 14
8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1525 1536
Size: 1525 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1536
Size: 340 1196
Bpu10I CC'TnA_GC
Cuts at: 0 22 119 1007 1536
Size: 22 97 888 529
Fragments arranged by size:
888 529 97 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 406 1536
Size: 64 342 1130
Fragments arranged by size:
1130 342 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 1199 1536
Size: 1199 337
BsaAI yAC'GTr
Cuts at: 0 484 1536
Size: 484 1052
BsaWI w'CCGG_w
Cuts at: 0 839 1536
Size: 839 697
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 511 541 1536
Size: 511 30 995
Fragments arranged by size:
995 511 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 611 1045 1536
Size: 21 590 434 491
Fragments arranged by size:
590 491 434 21
BseYI C'CCAG_C
Cuts at: 0 7 1345 1466 1536
Size: 7 1338 121 70
Fragments arranged by size:
1338 121 70 7
BsiHKAI G_wGCw'C
Cuts at: 0 586 1013 1536
Size: 586 427 523
Fragments arranged by size:
586 523 427
BsmI GAATG_Cn'
Cuts at: 0 1308 1536
Size: 1308 228
BsmBI CGTCTCn'nnnn_
Cuts at: 0 181 1536
Size: 181 1355
Bsp1286I G_dGCh'C
Cuts at: 0 8 222 395 586 879 1013 1145 1352
Size: 8 214 173 191 293 134 132 207
Cuts at: 1352 1522 1536
Size: 170 14
Fragments arranged by size:
293 214 207 191 173 170 134 132
14 8
BspHI T'CATG_A
Cuts at: 0 575 1536
Size: 575 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 101 1209 1536
Size: 101 1108 327
Fragments arranged by size:
1108 327 101
BsrBI CCG'CTC
Cuts at: 0 792 1536
Size: 792 744
BsrFI r'CCGG_y
Cuts at: 0 604 1190 1536
Size: 604 586 346
Fragments arranged by size:
604 586 346
BsrGI T'GTAC_A
Cuts at: 0 308 1536
Size: 308 1228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 364 1536
Size: 364 1172
BstYI r'GATC_y
Cuts at: 0 179 830 1043 1536
Size: 179 651 213 493
Fragments arranged by size:
651 493 213 179
Bsu36I CC'TnA_GG
Cuts at: 0 326 1268 1536
Size: 326 942 268
Fragments arranged by size:
942 326 268
BtgI C'CryG_G
Cuts at: 0 315 1536
Size: 315 1221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 560 1536
Size: 560 976
DrdI GACnn_nn'nnGTC
Cuts at: 0 1270 1536
Size: 1270 266
EaeI y'GGCC_r
Cuts at: 0 461 729 1188 1536
Size: 461 268 459 348
Fragments arranged by size:
461 459 348 268
EarI CTCTTCn'nnn_
Cuts at: 0 500 588 1536
Size: 500 88 948
Fragments arranged by size:
948 500 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1114 1536
Size: 1114 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 469 944 1528 1536
Size: 340 129 475 584 8
Fragments arranged by size:
584 475 340 129 8
EcoO109I rG'GnC_Cy
Cuts at: 0 142 321 330 391 1519 1536
Size: 142 179 9 61 1128 17
Fragments arranged by size:
1128 179 142 61 17 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 603 1137 1509 1536
Size: 603 534 372 27
Fragments arranged by size:
603 534 372 27
HaeII r_GCGC'y
Cuts at: 0 565 678 1536
Size: 565 113 858
Fragments arranged by size:
858 565 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 158 190 511 543 1019 1051 1177 1209
Size: 158 32 321 32 476 32 126 32
Cuts at: 1209 1291 1323 1536
Size: 82 32 213
Fragments arranged by size:
476 321 213 158 126 82 32 32
32 32 32
MslI CAynn'nnrTG
Cuts at: 0 440 574 937 1180 1229 1405 1459 1536
Size: 440 134 363 243 49 176 54 77
Fragments arranged by size:
440 363 243 176 134 77 54 49
NsiI A_TGCA'T
Cuts at: 0 1226 1308 1536
Size: 1226 82 228
Fragments arranged by size:
1226 228 82
NspI r_CATG'y
Cuts at: 0 172 1536
Size: 172 1364
PasI CC'CwG_GG
Cuts at: 0 139 1522 1536
Size: 139 1383 14
Fragments arranged by size:
1383 139 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 155 1536
Size: 155 1381
PfoI T'CCnGG_A
Cuts at: 0 182 857 1536
Size: 182 675 679
Fragments arranged by size:
679 675 182
PpuMI rG'GwC_Cy
Cuts at: 0 321 1536
Size: 321 1215
PspOMI G'GGCC_C
Cuts at: 0 218 391 1348 1518 1536
Size: 218 173 957 170 18
Fragments arranged by size:
957 218 173 170 18
PstI C_TGCA'G
Cuts at: 0 809 1206 1536
Size: 809 397 330
Fragments arranged by size:
809 397 330
SanDI GG'GwC_CC
Cuts at: 0 321 1536
Size: 321 1215
SbfI CC_TGCA'GG
Cuts at: 0 809 1206 1536
Size: 809 397 330
Fragments arranged by size:
809 397 330
SexAI A'CCwGG_T
Cuts at: 0 776 1536
Size: 776 760
SfcI C'TryA_G
Cuts at: 0 805 1202 1536
Size: 805 397 334
Fragments arranged by size:
805 397 334
SmaI CCC'GGG
Cuts at: 0 880 1536
Size: 880 656
SmlI C'TyrA_G
Cuts at: 0 79 175 385 1536
Size: 79 96 210 1151
Fragments arranged by size:
1151 210 96 79
StuI AGG'CCT
Cuts at: 0 374 1536
Size: 374 1162
StyI C'CwwG_G
Cuts at: 0 35 1047 1536
Size: 35 1012 489
Fragments arranged by size:
1012 489 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 257 862 1536
Size: 166 91 605 674
Fragments arranged by size:
674 605 166 91
TatI w'GTAC_w
Cuts at: 0 308 1536
Size: 308 1228
Tth111I GACn'n_nGTC
Cuts at: 0 778 1536
Size: 778 758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 160 1536
Size: 160 1376
XhoI C'TCGA_G
Cuts at: 0 175 1536
Size: 175 1361
XmaI C'CCGG_G
Cuts at: 0 878 1536
Size: 878 658
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI AvrII BanI BanII BcgI BfrBI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BstXI BstYI Bsu36I
BtgI BtgZI DrdI EaeI EarI EciI Eco57MI EcoO109I
FalI HaeII Hin4I MslI NsiI NspI PasI PflMI
PfoI PpuMI PspOMI PstI SanDI SbfI SexAI SfcI
SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI
XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
AscI AseI AsiSI BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BglI BglII BmgBI BmtI BplI BsaBI
BsaHI BsgI BsiEI BsiWI BspEI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI
DraIII EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XmnI ZraI