Restriction map for
pFN21AE4491
(Linear) (Six-base) MAPSORT of: KIEE4491.seq Check: 8995 from: 1 to: 1536
KIEE4491.orf
With 182 enzymes: SgfI *
March 22, 2012 18:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 351 1536
Size: 130 221 1185
Fragments arranged by size:
1185 221 130
AhdI GACnn_n'nnGTC
Cuts at: 0 94 1536
Size: 94 1442
AleI CACnn'nnGTG
Cuts at: 0 244 1536
Size: 244 1292
AlwNI CAG_nnn'CTG
Cuts at: 0 285 379 528 541 1536
Size: 285 94 149 13 995
Fragments arranged by size:
995 285 149 94 13
ApoI r'AATT_y
Cuts at: 0 1435 1536
Size: 1435 101
BanI G'GyrC_C
Cuts at: 0 404 1536
Size: 404 1132
BbeI G_GCGC'C
Cuts at: 0 408 1536
Size: 408 1128
BbvCI CC'TCA_GC
Cuts at: 0 500 1536
Size: 500 1036
BlpI GC'TnA_GC
Cuts at: 0 256 643 1536
Size: 256 387 893
Fragments arranged by size:
893 387 256
Bme1580I G_kGCm'C
Cuts at: 0 152 548 1536
Size: 152 396 988
Fragments arranged by size:
988 396 152
BmgBI CAC'GTC
Cuts at: 0 216 1536
Size: 216 1320
BmrI ACTGGGnnnn_n'
Cuts at: 0 1459 1536
Size: 1459 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1002 1138 1536
Size: 1002 136 398
Fragments arranged by size:
1002 398 136
Bpu10I CC'TnA_GC
Cuts at: 0 500 1536
Size: 500 1036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 1536
Size: 69 1467
BsaI GGTCTCn'nnnn_
Cuts at: 0 1114 1372 1536
Size: 1114 258 164
Fragments arranged by size:
1114 258 164
BsaHI Gr'CG_yC
Cuts at: 0 405 1536
Size: 405 1131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 1536
Size: 439 30 1067
Fragments arranged by size:
1067 439 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 447 1048 1536
Size: 447 601 488
Fragments arranged by size:
601 488 447
BseYI C'CCAG_C
Cuts at: 0 450 696 949 1391 1536
Size: 450 246 253 442 145
Fragments arranged by size:
450 442 253 246 145
BsiHKAI G_wGCw'C
Cuts at: 0 806 863 1514 1536
Size: 806 57 651 22
Fragments arranged by size:
806 651 57 22
BsmI GAATG_Cn'
Cuts at: 0 750 1536
Size: 750 786
Bsp1286I G_dGCh'C
Cuts at: 0 152 548 806 863 1514 1536
Size: 152 396 258 57 651 22
Fragments arranged by size:
651 396 258 152 57 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 1536
Size: 1046 490
BsrBI CCG'CTC
Cuts at: 0 260 1536
Size: 260 1276
BsrDI GCAATG_nn'
Cuts at: 0 437 1382 1536
Size: 437 945 154
Fragments arranged by size:
945 437 154
BsrFI r'CCGG_y
Cuts at: 0 314 401 611 1536
Size: 314 87 210 925
Fragments arranged by size:
925 314 210 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 128 134 208 246 1536
Size: 128 6 74 38 1290
Fragments arranged by size:
1290 128 74 38 6
BstYI r'GATC_y
Cuts at: 0 224 1025 1084 1536
Size: 224 801 59 452
Fragments arranged by size:
801 452 224 59
Bsu36I CC'TnA_GG
Cuts at: 0 92 1536
Size: 92 1444
BtgI C'CryG_G
Cuts at: 0 26 127 245 1536
Size: 26 101 118 1291
Fragments arranged by size:
1291 118 101 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 314 1536
Size: 314 1222
EaeI y'GGCC_r
Cuts at: 0 23 609 1536
Size: 23 586 927
Fragments arranged by size:
927 586 23
EarI CTCTTCn'nnn_
Cuts at: 0 473 1140 1411 1536
Size: 473 667 271 125
Fragments arranged by size:
667 473 271 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 351 1002 1138 1536
Size: 130 221 651 136 398
Fragments arranged by size:
651 398 221 136 130
EcoO109I rG'GnC_Cy
Cuts at: 0 80 96 282 504 538 756 1536
Size: 80 16 186 222 34 218 780
Fragments arranged by size:
780 222 218 186 80 34 16
EcoRI G'AATT_C
Cuts at: 0 1435 1536
Size: 1435 101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 658 1536
Size: 658 878
FseI GG_CCGG'CC
Cuts at: 0 318 1536
Size: 318 1218
HaeII r_GCGC'y
Cuts at: 0 408 618 1536
Size: 408 210 918
Fragments arranged by size:
918 408 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 80 112 439 471 1536
Size: 80 32 327 32 1065
Fragments arranged by size:
1065 327 80 32 32
HincII GTy'rAC
Cuts at: 0 654 1266 1536
Size: 654 612 270
Fragments arranged by size:
654 612 270
KasI G'GCGC_C
Cuts at: 0 404 1536
Size: 404 1132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1152 1536
Size: 1152 384
MslI CAynn'nnrTG
Cuts at: 0 244 1536
Size: 244 1292
MspA1I CmG'CkG
Cuts at: 0 700 879 903 1391 1527 1536
Size: 700 179 24 488 136 9
Fragments arranged by size:
700 488 179 136 24 9
NaeI GCC'GGC
Cuts at: 0 316 403 613 1536
Size: 316 87 210 923
Fragments arranged by size:
923 316 210 87
NarI GG'CG_CC
Cuts at: 0 405 1536
Size: 405 1131
NcoI C'CATG_G
Cuts at: 0 127 1536
Size: 127 1409
NgoMIV G'CCGG_C
Cuts at: 0 314 401 611 1536
Size: 314 87 210 925
Fragments arranged by size:
925 314 210 87
NspI r_CATG'y
Cuts at: 0 156 363 487 1501 1536
Size: 156 207 124 1014 35
Fragments arranged by size:
1014 207 156 124 35
PasI CC'CwG_GG
Cuts at: 0 162 1010 1536
Size: 162 848 526
Fragments arranged by size:
848 526 162
PflMI CCAn_nnn'nTGG
Cuts at: 0 379 1536
Size: 379 1157
PfoI T'CCnGG_A
Cuts at: 0 833 979 1115 1536
Size: 833 146 136 421
Fragments arranged by size:
833 421 146 136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1280 1312 1536
Size: 1280 32 224
Fragments arranged by size:
1280 224 32
PpuMI rG'GwC_Cy
Cuts at: 0 96 504 756 1536
Size: 96 408 252 780
Fragments arranged by size:
780 408 252 96
PshAI GACnn'nnGTC
Cuts at: 0 586 1536
Size: 586 950
PstI C_TGCA'G
Cuts at: 0 224 1536
Size: 224 1312
PvuII CAG'CTG
Cuts at: 0 700 879 903 1527 1536
Size: 700 179 24 624 9
Fragments arranged by size:
700 624 179 24 9
SapI GCTCTTCn'nnn_
Cuts at: 0 473 1536
Size: 473 1063
SfcI C'TryA_G
Cuts at: 0 220 558 774 1536
Size: 220 338 216 762
Fragments arranged by size:
762 338 220 216
SfoI GGC'GCC
Cuts at: 0 406 1536
Size: 406 1130
SgrAI Cr'CCGG_yG
Cuts at: 0 401 1536
Size: 401 1135
SmlI C'TyrA_G
Cuts at: 0 84 1536
Size: 84 1452
SphI G_CATG'C
Cuts at: 0 1501 1536
Size: 1501 35
StuI AGG'CCT
Cuts at: 0 107 1108 1536
Size: 107 1001 428
Fragments arranged by size:
1001 428 107
StyI C'CwwG_G
Cuts at: 0 127 1205 1536
Size: 127 1078 331
Fragments arranged by size:
1078 331 127
Tth111I GACn'n_nGTC
Cuts at: 0 961 1536
Size: 961 575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 125 246 1536
Size: 125 121 1290
Fragments arranged by size:
1290 125 121
XmnI GAAnn'nnTTC
Cuts at: 0 430 748 1292 1536
Size: 430 318 544 244
Fragments arranged by size:
544 430 318 244
Enzymes that do cut:
AcuI AhdI AleI AlwNI ApoI BanI BbeI BbvCI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtgZI
EaeI EarI Eco57MI EcoO109I EcoRI FalI FseI HaeII
Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI
PshAI PstI PvuII SapI SfcI SfoI SgrAI SmlI
SphI StuI StyI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BmtI BplI BsaAI BsaBI
BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI
MscI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SmaI SnaBI SpeI SrfI SspI SwaI TaqII
TatI TstI XbaI XhoI XmaI ZraI