Restriction map for
pFN21AE4488
(Linear) (Six-base) MAPSORT of: KIEE4488.seq Check: 9725 from: 1 to: 1530
KIEE4488.orf
With 182 enzymes: SgfI *
March 22, 2012 18:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1334 1530
Size: 1334 196
AccI GT'mk_AC
Cuts at: 0 1429 1530
Size: 1429 101
ApaI G_GGCC'C
Cuts at: 0 204 406 1530
Size: 204 202 1124
Fragments arranged by size:
1124 204 202
ApoI r'AATT_y
Cuts at: 0 236 682 839 874 916 1221 1522 1530
Size: 236 446 157 35 42 305 301 8
Fragments arranged by size:
446 305 301 236 157 42 35 8
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1160 1193 1530
Size: 1160 33 337
Fragments arranged by size:
1160 337 33
BamHI G'GATC_C
Cuts at: 0 7 1530
Size: 7 1523
BanII G_rGCy'C
Cuts at: 0 204 406 1530
Size: 204 202 1124
Fragments arranged by size:
1124 204 202
BciVI GTATCCnnnnn_n'
Cuts at: 0 1161 1530
Size: 1161 369
BclI T'GATC_A
Cuts at: 0 644 1530
Size: 644 886
BglI GCCn_nnn'nGGC
Cuts at: 0 269 1530
Size: 269 1261
Bme1580I G_kGCm'C
Cuts at: 0 194 204 406 1530
Size: 194 10 202 1124
Fragments arranged by size:
1124 202 194 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 318 1530
Size: 318 1212
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1125 1530
Size: 1125 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1298 1530
Size: 770 528 232
Fragments arranged by size:
770 528 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1248 1530
Size: 1248 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 28 98 1502 1530
Size: 28 70 1404 28
Fragments arranged by size:
1404 70 28 28
BsaHI Gr'CG_yC
Cuts at: 0 1283 1530
Size: 1283 247
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 116 1133 1530
Size: 116 1017 397
Fragments arranged by size:
1017 397 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 701 1312 1381 1530
Size: 193 508 611 69 149
Fragments arranged by size:
611 508 193 149 69
BsiEI CG_ry'CG
Cuts at: 0 43 75 116 1530
Size: 43 32 41 1414
Fragments arranged by size:
1414 43 41 32
BsiHKAI G_wGCw'C
Cuts at: 0 884 1325 1530
Size: 884 441 205
Fragments arranged by size:
884 441 205
BsmI GAATG_Cn'
Cuts at: 0 219 1133 1262 1530
Size: 219 914 129 268
Fragments arranged by size:
914 268 219 129
Bsp1286I G_dGCh'C
Cuts at: 0 194 204 406 884 1325 1530
Size: 194 10 202 478 441 205
Fragments arranged by size:
478 441 205 202 194 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1334 1530
Size: 1334 196
BsrFI r'CCGG_y
Cuts at: 0 1046 1530
Size: 1046 484
BstAPI GCAn_nnn'nTGC
Cuts at: 0 171 1530
Size: 171 1359
BstYI r'GATC_y
Cuts at: 0 7 1530
Size: 7 1523
BtgI C'CryG_G
Cuts at: 0 110 1530
Size: 110 1420
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 112 1530
Size: 112 1418
DraI TTT'AAA
Cuts at: 0 257 1530
Size: 257 1273
DraIII CAC_nnn'GTG
Cuts at: 0 171 1530
Size: 171 1359
DrdI GACnn_nn'nnGTC
Cuts at: 0 112 772 1530
Size: 112 660 758
Fragments arranged by size:
758 660 112
EaeI y'GGCC_r
Cuts at: 0 40 1530
Size: 40 1490
EagI C'GGCC_G
Cuts at: 0 40 1530
Size: 40 1490
EarI CTCTTCn'nnn_
Cuts at: 0 336 1095 1530
Size: 336 759 435
Fragments arranged by size:
759 435 336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1298 1530
Size: 770 528 232
Fragments arranged by size:
770 528 232
EcoNI CCTnn'n_nnAGG
Cuts at: 0 719 1530
Size: 719 811
EcoO109I rG'GnC_Cy
Cuts at: 0 402 403 1530
Size: 402 1 1127
Fragments arranged by size:
1127 402 1
EcoRI G'AATT_C
Cuts at: 0 916 1530
Size: 916 614
HincII GTy'rAC
Cuts at: 0 800 1530
Size: 800 730
HindIII A'AGCT_T
Cuts at: 0 554 1530
Size: 554 976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 1530
Size: 420 1110
MslI CAynn'nnrTG
Cuts at: 0 536 1530
Size: 536 994
MspA1I CmG'CkG
Cuts at: 0 45 112 421 1041 1513 1530
Size: 45 67 309 620 472 17
Fragments arranged by size:
620 472 309 67 45 17
PspOMI G'GGCC_C
Cuts at: 0 200 402 1530
Size: 200 202 1128
Fragments arranged by size:
1128 202 200
PvuII CAG'CTG
Cuts at: 0 421 1041 1513 1530
Size: 421 620 472 17
Fragments arranged by size:
620 472 421 17
SacII CC_GC'GG
Cuts at: 0 113 1530
Size: 113 1417
SexAI A'CCwGG_T
Cuts at: 0 167 1530
Size: 167 1363
SfcI C'TryA_G
Cuts at: 0 491 1530
Size: 491 1039
SmlI C'TyrA_G
Cuts at: 0 1227 1530
Size: 1227 303
SspI AAT'ATT
Cuts at: 0 446 965 1530
Size: 446 519 565
Fragments arranged by size:
565 519 446
StyI C'CwwG_G
Cuts at: 0 942 1530
Size: 942 588
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 61 1530
Size: 61 1469
TatI w'GTAC_w
Cuts at: 0 140 1530
Size: 140 1390
Enzymes that do cut:
AarI AccI ApaI ApoI BaeI BamHI BanII BciVI
BclI BglI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaHI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrFI BstAPI BstYI BtgI BtgZI DraI DraIII DrdI
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI HincII
HindIII MmeI MslI MspA1I PspOMI PvuII SacII SexAI
SfcI SmlI SspI StyI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BanI BbeI BbsI BbvCI BcgI
BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaBI
BsaWI BsaXI BseYI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI