Restriction map for
pFN21AE4487
(Linear) (Six-base) MAPSORT of: KIEE4487.seq Check: 4265 from: 1 to: 1524
KIEE4487.orf
With 182 enzymes: SgfI *
March 22, 2012 18:11 ..
AatII G_ACGT'C
Cuts at: 0 1163 1524
Size: 1163 361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 1524
Size: 541 983
AlwNI CAG_nnn'CTG
Cuts at: 0 78 303 363 1524
Size: 78 225 60 1161
Fragments arranged by size:
1161 225 78 60
ApoI r'AATT_y
Cuts at: 0 157 799 992 1047 1354 1524
Size: 157 642 193 55 307 170
Fragments arranged by size:
642 307 193 170 157 55
AseI AT'TA_AT
Cuts at: 0 488 1524
Size: 488 1036
BanII G_rGCy'C
Cuts at: 0 8 518 1524
Size: 8 510 1006
Fragments arranged by size:
1006 510 8
BbvCI CC'TCA_GC
Cuts at: 0 1459 1524
Size: 1459 65
BciVI GTATCCnnnnn_n'
Cuts at: 0 863 1524
Size: 863 661
BglII A'GATC_T
Cuts at: 0 434 1524
Size: 434 1090
BmrI ACTGGGnnnn_n'
Cuts at: 0 631 1524
Size: 631 893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 1524
Size: 100 1424
Bpu10I CC'TnA_GC
Cuts at: 0 1459 1524
Size: 1459 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1524
Size: 235 1289
BsaI GGTCTCn'nnnn_
Cuts at: 0 1399 1524
Size: 1399 125
BsaBI GATnn'nnATC
Cuts at: 0 962 1524
Size: 962 562
BsaHI Gr'CG_yC
Cuts at: 0 1160 1524
Size: 1160 364
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1379 1409 1524
Size: 1379 30 115
Fragments arranged by size:
1379 115 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1214 1476 1524
Size: 1214 262 48
Fragments arranged by size:
1214 262 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1524
Size: 121 1403
BsiEI CG_ry'CG
Cuts at: 0 45 49 1524
Size: 45 4 1475
Fragments arranged by size:
1475 45 4
BsiHKAI G_wGCw'C
Cuts at: 0 8 266 518 1524
Size: 8 258 252 1006
Fragments arranged by size:
1006 258 252 8
Bsp1286I G_dGCh'C
Cuts at: 0 8 266 518 1524
Size: 8 258 252 1006
Fragments arranged by size:
1006 258 252 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 1524
Size: 727 797
BsrDI GCAATG_nn'
Cuts at: 0 722 1161 1524
Size: 722 439 363
Fragments arranged by size:
722 439 363
BsrFI r'CCGG_y
Cuts at: 0 44 1524
Size: 44 1480
BsrGI T'GTAC_A
Cuts at: 0 167 1524
Size: 167 1357
BstAPI GCAn_nnn'nTGC
Cuts at: 0 388 1524
Size: 388 1136
BstXI CCAn_nnnn'nTGG
Cuts at: 0 438 1218 1524
Size: 438 780 306
Fragments arranged by size:
780 438 306
BstYI r'GATC_y
Cuts at: 0 434 471 1524
Size: 434 37 1053
Fragments arranged by size:
1053 434 37
BtgI C'CryG_G
Cuts at: 0 509 1524
Size: 509 1015
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 117 360 1524
Size: 117 243 1164
Fragments arranged by size:
1164 243 117
BtsI GCAGTG_nn'
Cuts at: 0 557 1524
Size: 557 967
DraI TTT'AAA
Cuts at: 0 1289 1524
Size: 1289 235
EaeI y'GGCC_r
Cuts at: 0 42 128 711 1524
Size: 42 86 583 813
Fragments arranged by size:
813 583 86 42
EagI C'GGCC_G
Cuts at: 0 42 1524
Size: 42 1482
EcoICRI GAG'CTC
Cuts at: 0 6 516 1524
Size: 6 510 1008
Fragments arranged by size:
1008 510 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 541 1524
Size: 100 441 983
Fragments arranged by size:
983 441 100
EcoO109I rG'GnC_Cy
Cuts at: 0 84 1470 1524
Size: 84 1386 54
Fragments arranged by size:
1386 84 54
EcoRI G'AATT_C
Cuts at: 0 157 1524
Size: 157 1367
EcoRV GAT'ATC
Cuts at: 0 120 1318 1524
Size: 120 1198 206
Fragments arranged by size:
1198 206 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 834 866 1117 1149 1524
Size: 834 32 251 32 375
Fragments arranged by size:
834 375 251 32 32
HindIII A'AGCT_T
Cuts at: 0 445 1524
Size: 445 1079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 1524
Size: 48 1476
MscI TGG'CCA
Cuts at: 0 713 1524
Size: 713 811
MspA1I CmG'CkG
Cuts at: 0 78 1429 1524
Size: 78 1351 95
Fragments arranged by size:
1351 95 78
NruI TCG'CGA
Cuts at: 0 51 1524
Size: 51 1473
PsiI TTA'TAA
Cuts at: 0 400 1524
Size: 400 1124
PstI C_TGCA'G
Cuts at: 0 74 384 1524
Size: 74 310 1140
Fragments arranged by size:
1140 310 74
PvuII CAG'CTG
Cuts at: 0 78 1524
Size: 78 1446
SacI G_AGCT'C
Cuts at: 0 8 518 1524
Size: 8 510 1006
Fragments arranged by size:
1006 510 8
ScaI AGT'ACT
Cuts at: 0 950 1524
Size: 950 574
SfcI C'TryA_G
Cuts at: 0 70 380 1524
Size: 70 310 1144
Fragments arranged by size:
1144 310 70
SmlI C'TyrA_G
Cuts at: 0 214 1524
Size: 214 1310
StuI AGG'CCT
Cuts at: 0 283 1524
Size: 283 1241
StyI C'CwwG_G
Cuts at: 0 192 974 1524
Size: 192 782 550
Fragments arranged by size:
782 550 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1381 1524
Size: 1381 143
TatI w'GTAC_w
Cuts at: 0 167 948 1524
Size: 167 781 576
Fragments arranged by size:
781 576 167
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 377 409 1524
Size: 377 32 1115
Fragments arranged by size:
1115 377 32
XmnI GAAnn'nnTTC
Cuts at: 0 1372 1524
Size: 1372 152
ZraI GAC'GTC
Cuts at: 0 1161 1524
Size: 1161 363
Enzymes that do cut:
AatII AcuI AlwNI ApoI AseI BanII BbvCI BciVI
BglII BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI
DraI EaeI EagI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
Hin4I HindIII MmeI MscI MspA1I NruI PsiI PstI
PvuII SacI ScaI SfcI SmlI StuI StyI TaqII
TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI
BsaAI BsaWI BseYI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraIII DrdI EarI EciI EcoNI FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI