Restriction map for
pFN21AE4486
(Linear) (Six-base) MAPSORT of: KIEE4486.seq Check: 7813 from: 1 to: 1506
KIEE4486.orf
With 182 enzymes: SgfI *
March 22, 2012 18:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1360 1506
Size: 799 561 146
Fragments arranged by size:
799 561 146
AlwNI CAG_nnn'CTG
Cuts at: 0 990 1231 1319 1506
Size: 990 241 88 187
Fragments arranged by size:
990 241 187 88
ApaI G_GGCC'C
Cuts at: 0 122 1506
Size: 122 1384
ApoI r'AATT_y
Cuts at: 0 15 110 175 634 738 837 1506
Size: 15 95 65 459 104 99 669
Fragments arranged by size:
669 459 104 99 95 65 15
BanI G'GyrC_C
Cuts at: 0 1026 1506
Size: 1026 480
BanII G_rGCy'C
Cuts at: 0 122 884 1506
Size: 122 762 622
Fragments arranged by size:
762 622 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 1506
Size: 294 1212
BbvCI CC'TCA_GC
Cuts at: 0 362 1506
Size: 362 1144
BclI T'GATC_A
Cuts at: 0 266 1506
Size: 266 1240
Bme1580I G_kGCm'C
Cuts at: 0 122 1506
Size: 122 1384
BmgBI CAC'GTC
Cuts at: 0 264 1506
Size: 264 1242
BmrI ACTGGGnnnn_n'
Cuts at: 0 115 322 444 685 885 1096 1506
Size: 115 207 122 241 200 211 410
Fragments arranged by size:
410 241 211 207 200 122 115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1355 1506
Size: 1355 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1506
Size: 898 608
Bpu10I CC'TnA_GC
Cuts at: 0 362 1506
Size: 362 1144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 498 1138 1506
Size: 409 89 640 368
Fragments arranged by size:
640 409 368 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 172 1506
Size: 172 1334
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 1249 1506
Size: 52 1197 257
Fragments arranged by size:
1197 257 52
BsiHKAI G_wGCw'C
Cuts at: 0 62 619 884 1506
Size: 62 557 265 622
Fragments arranged by size:
622 557 265 62
Bsp1286I G_dGCh'C
Cuts at: 0 62 122 619 884 1506
Size: 62 60 497 265 622
Fragments arranged by size:
622 497 265 62 60
BspHI T'CATG_A
Cuts at: 0 1343 1506
Size: 1343 163
BsrDI GCAATG_nn'
Cuts at: 0 391 443 1506
Size: 391 52 1063
Fragments arranged by size:
1063 391 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 990 1506
Size: 990 516
BstBI TT'CG_AA
Cuts at: 0 841 1506
Size: 841 665
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1220 1506
Size: 1220 286
BstYI r'GATC_y
Cuts at: 0 255 648 1278 1412 1506
Size: 255 393 630 134 94
Fragments arranged by size:
630 393 255 134 94
Bsu36I CC'TnA_GG
Cuts at: 0 1232 1506
Size: 1232 274
BtgI C'CryG_G
Cuts at: 0 1046 1506
Size: 1046 460
BtsI GCAGTG_nn'
Cuts at: 0 939 1506
Size: 939 567
DraIII CAC_nnn'GTG
Cuts at: 0 188 1506
Size: 188 1318
EaeI y'GGCC_r
Cuts at: 0 245 962 1506
Size: 245 717 544
Fragments arranged by size:
717 544 245
EarI CTCTTCn'nnn_
Cuts at: 0 373 1205 1506
Size: 373 832 301
Fragments arranged by size:
832 373 301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1231 1506
Size: 1231 275
EcoICRI GAG'CTC
Cuts at: 0 882 1506
Size: 882 624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 898 1360 1506
Size: 799 99 462 146
Fragments arranged by size:
799 462 146 99
EcoO109I rG'GnC_Cy
Cuts at: 0 77 118 530 1506
Size: 77 41 412 976
Fragments arranged by size:
976 412 77 41
EcoRI G'AATT_C
Cuts at: 0 110 837 1506
Size: 110 727 669
Fragments arranged by size:
727 669 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1459 1506
Size: 1459 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 560 592 1506
Size: 560 32 914
Fragments arranged by size:
914 560 32
HincII GTy'rAC
Cuts at: 0 232 1506
Size: 232 1274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1213 1506
Size: 427 786 293
Fragments arranged by size:
786 427 293
MscI TGG'CCA
Cuts at: 0 247 964 1506
Size: 247 717 542
Fragments arranged by size:
717 542 247
MslI CAynn'nnrTG
Cuts at: 0 544 974 1075 1327 1348 1506
Size: 544 430 101 252 21 158
Fragments arranged by size:
544 430 252 158 101 21
MspA1I CmG'CkG
Cuts at: 0 416 819 1506
Size: 416 403 687
Fragments arranged by size:
687 416 403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 1506
Size: 565 32 909
Fragments arranged by size:
909 565 32
PpuMI rG'GwC_Cy
Cuts at: 0 77 1506
Size: 77 1429
PspOMI G'GGCC_C
Cuts at: 0 118 1506
Size: 118 1388
PvuII CAG'CTG
Cuts at: 0 819 1506
Size: 819 687
SacI G_AGCT'C
Cuts at: 0 884 1506
Size: 884 622
SanDI GG'GwC_CC
Cuts at: 0 77 1506
Size: 77 1429
SapI GCTCTTCn'nnn_
Cuts at: 0 1205 1506
Size: 1205 301
ScaI AGT'ACT
Cuts at: 0 1219 1506
Size: 1219 287
SfcI C'TryA_G
Cuts at: 0 534 1506
Size: 534 972
SmlI C'TyrA_G
Cuts at: 0 424 477 1117 1506
Size: 424 53 640 389
Fragments arranged by size:
640 424 389 53
TatI w'GTAC_w
Cuts at: 0 1217 1506
Size: 1217 289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1173 1506
Size: 1173 333
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BanI BanII BbsI BbvCI
BclI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BseRI BsiHKAI Bsp1286I BspHI BsrDI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HincII
MmeI MscI MslI MspA1I PpiI PpuMI PspOMI PvuII
SacI SanDI SapI ScaI SfcI SmlI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI
BfrBI BglI BglII BlpI BmtI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstEII
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoNI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI