Restriction map for
pFN21AE4477
(Linear) (Six-base) MAPSORT of: KIEE4477.seq Check: 7762 from: 1 to: 1485
KIEE4477.orf
With 182 enzymes: SgfI *
March 22, 2012 18:09 ..
AccI GT'mk_AC
Cuts at: 0 200 1485
Size: 200 1285
AfeI AGC'GCT
Cuts at: 0 934 1485
Size: 934 551
AflIII A'CryG_T
Cuts at: 0 1289 1485
Size: 1289 196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1252 1485
Size: 1252 233
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1399 1431 1485
Size: 1399 32 54
Fragments arranged by size:
1399 54 32
AlwNI CAG_nnn'CTG
Cuts at: 0 705 1344 1485
Size: 705 639 141
Fragments arranged by size:
705 639 141
ApaI G_GGCC'C
Cuts at: 0 108 116 1424 1485
Size: 108 8 1308 61
Fragments arranged by size:
1308 108 61 8
ApoI r'AATT_y
Cuts at: 0 96 1485
Size: 96 1389
AscI GG'CGCG_CC
Cuts at: 0 727 1485
Size: 727 758
AvaI C'yCGr_G
Cuts at: 0 101 107 190 1024 1324 1423 1485
Size: 101 6 83 834 300 99 62
Fragments arranged by size:
834 300 101 99 83 62 6
AvrII C'CTAG_G
Cuts at: 0 91 1485
Size: 91 1394
BanI G'GyrC_C
Cuts at: 0 186 408 500 633 655 1347 1380 1485
Size: 186 222 92 133 22 692 33 105
Fragments arranged by size:
692 222 186 133 105 92 33 22
BanII G_rGCy'C
Cuts at: 0 108 116 1412 1424 1485
Size: 108 8 1296 12 61
Fragments arranged by size:
1296 108 61 12 8
BbeI G_GCGC'C
Cuts at: 0 637 1384 1485
Size: 637 747 101
Fragments arranged by size:
747 637 101
BbvCI CC'TCA_GC
Cuts at: 0 404 1485
Size: 404 1081
BglI GCCn_nnn'nGGC
Cuts at: 0 31 1485
Size: 31 1454
BlpI GC'TnA_GC
Cuts at: 0 770 1485
Size: 770 715
Bme1580I G_kGCm'C
Cuts at: 0 108 116 191 658 1352 1424 1485
Size: 108 8 75 467 694 72 61
Fragments arranged by size:
694 467 108 75 72 61 8
BmgBI CAC'GTC
Cuts at: 0 460 1086 1113 1485
Size: 460 626 27 372
Fragments arranged by size:
626 460 372 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 1437 1485
Size: 1437 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1050 1167 1485
Size: 1050 117 318
Fragments arranged by size:
1050 318 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 442 871 1018 1426 1485
Size: 295 147 429 147 408 59
Fragments arranged by size:
429 408 295 147 147 59
Bpu10I CC'TnA_GC
Cuts at: 0 404 1485
Size: 404 1081
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 1485
Size: 1396 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 868 964 1485
Size: 346 522 96 521
Fragments arranged by size:
522 521 346 96
BsaAI yAC'GTr
Cuts at: 0 360 1242 1292 1485
Size: 360 882 50 193
Fragments arranged by size:
882 360 193 50
BsaHI Gr'CG_yC
Cuts at: 0 575 634 940 1274 1381 1485
Size: 575 59 306 334 107 104
Fragments arranged by size:
575 334 306 107 104 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 578 608 1399 1429 1485
Size: 578 30 791 30 56
Fragments arranged by size:
791 578 56 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 943 1485
Size: 943 542
BseYI C'CCAG_C
Cuts at: 0 222 227 689 813 833 1041 1485
Size: 222 5 462 124 20 208 444
Fragments arranged by size:
462 444 222 208 124 20 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1234 1485
Size: 718 516 251
Fragments arranged by size:
718 516 251
BsiEI CG_ry'CG
Cuts at: 0 35 649 1193 1485
Size: 35 614 544 292
Fragments arranged by size:
614 544 292 35
BsiHKAI G_wGCw'C
Cuts at: 0 602 1331 1412 1485
Size: 602 729 81 73
Fragments arranged by size:
729 602 81 73
Bsp1286I G_dGCh'C
Cuts at: 0 108 116 191 602 658 1331 1352 1412
Size: 108 8 75 411 56 673 21 60
Cuts at: 1412 1424 1485
Size: 12 61
Fragments arranged by size:
673 411 108 75 61 60 56 21
12 8
BspHI T'CATG_A
Cuts at: 0 1277 1485
Size: 1277 208
BsrDI GCAATG_nn'
Cuts at: 0 1275 1485
Size: 1275 210
BsrFI r'CCGG_y
Cuts at: 0 133 326 648 1147 1304 1363 1485
Size: 133 193 322 499 157 59 122
Fragments arranged by size:
499 322 193 157 133 122 59
BssHII G'CGCG_C
Cuts at: 0 606 727 757 809 957 959 1485
Size: 606 121 30 52 148 2 526
Fragments arranged by size:
606 526 148 121 52 30 2
BssSI C'ACGA_G
Cuts at: 0 72 1485
Size: 72 1413
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1335 1485
Size: 1335 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 1485
Size: 504 981
BtgI C'CryG_G
Cuts at: 0 233 482 722 746 896 1056 1072 1485
Size: 233 249 240 24 150 160 16 413
Fragments arranged by size:
413 249 240 233 160 150 24 16
BtsI GCAGTG_nn'
Cuts at: 0 1036 1459 1485
Size: 1036 423 26
Fragments arranged by size:
1036 423 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 354 389 1485
Size: 354 35 1096
Fragments arranged by size:
1096 354 35
EaeI y'GGCC_r
Cuts at: 0 32 328 362 467 485 646 743 1485
Size: 32 296 34 105 18 161 97 742
Fragments arranged by size:
742 296 161 105 97 34 32 18
EagI C'GGCC_G
Cuts at: 0 32 646 1485
Size: 32 614 839
Fragments arranged by size:
839 614 32
EarI CTCTTCn'nnn_
Cuts at: 0 67 625 1485
Size: 67 558 860
Fragments arranged by size:
860 558 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 811 1485
Size: 811 674
EcoICRI GAG'CTC
Cuts at: 0 1410 1485
Size: 1410 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 442 871 1018 1426 1485
Size: 295 147 429 147 408 59
Fragments arranged by size:
429 408 295 147 147 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 925 1485
Size: 925 560
EcoO109I rG'GnC_Cy
Cuts at: 0 112 113 1485
Size: 112 1 1372
Fragments arranged by size:
1372 112 1
EcoRI G'AATT_C
Cuts at: 0 96 1485
Size: 96 1389
HaeII r_GCGC'y
Cuts at: 0 307 442 637 802 936 1384 1485
Size: 307 135 195 165 134 448 101
Fragments arranged by size:
448 307 195 165 135 134 101
KasI G'GCGC_C
Cuts at: 0 633 1380 1485
Size: 633 747 105
Fragments arranged by size:
747 633 105
MscI TGG'CCA
Cuts at: 0 364 745 1485
Size: 364 381 740
Fragments arranged by size:
740 381 364
MspA1I CmG'CkG
Cuts at: 0 14 569 702 724 741 1131 1344 1485
Size: 14 555 133 22 17 390 213 141
Fragments arranged by size:
555 390 213 141 133 22 17 14
NaeI GCC'GGC
Cuts at: 0 135 1149 1365 1485
Size: 135 1014 216 120
Fragments arranged by size:
1014 216 135 120
NarI GG'CG_CC
Cuts at: 0 634 1381 1485
Size: 634 747 104
Fragments arranged by size:
747 634 104
NcoI C'CATG_G
Cuts at: 0 1072 1485
Size: 1072 413
NgoMIV G'CCGG_C
Cuts at: 0 133 1147 1363 1485
Size: 133 1014 216 122
Fragments arranged by size:
1014 216 133 122
NotI GC'GGCC_GC
Cuts at: 0 32 1485
Size: 32 1453
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 1485
Size: 234 1251
PfoI T'CCnGG_A
Cuts at: 0 1403 1485
Size: 1403 82
PmlI CAC'GTG
Cuts at: 0 1242 1292 1485
Size: 1242 50 193
Fragments arranged by size:
1242 193 50
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1399 1431 1485
Size: 1399 32 54
Fragments arranged by size:
1399 54 32
PspOMI G'GGCC_C
Cuts at: 0 104 112 1420 1485
Size: 104 8 1308 65
Fragments arranged by size:
1308 104 65 8
PspXI vC'TCGA_Gb
Cuts at: 0 1024 1485
Size: 1024 461
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1429 1461 1485
Size: 1429 32 24
Fragments arranged by size:
1429 32 24
PstI C_TGCA'G
Cuts at: 0 83 215 674 1340 1485
Size: 83 132 459 666 145
Fragments arranged by size:
666 459 145 132 83
PvuII CAG'CTG
Cuts at: 0 702 1131 1485
Size: 702 429 354
Fragments arranged by size:
702 429 354
SacI G_AGCT'C
Cuts at: 0 1412 1485
Size: 1412 73
SacII CC_GC'GG
Cuts at: 0 725 1485
Size: 725 760
SfcI C'TryA_G
Cuts at: 0 79 211 670 1336 1485
Size: 79 132 459 666 149
Fragments arranged by size:
666 459 149 132 79
SfoI GGC'GCC
Cuts at: 0 635 1382 1485
Size: 635 747 103
Fragments arranged by size:
747 635 103
SmaI CCC'GGG
Cuts at: 0 103 109 1425 1485
Size: 103 6 1316 60
Fragments arranged by size:
1316 103 60 6
SmlI C'TyrA_G
Cuts at: 0 1024 1411 1485
Size: 1024 387 74
Fragments arranged by size:
1024 387 74
StyI C'CwwG_G
Cuts at: 0 91 1072 1283 1485
Size: 91 981 211 202
Fragments arranged by size:
981 211 202 91
TatI w'GTAC_w
Cuts at: 0 1217 1485
Size: 1217 268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 884 916 1485
Size: 578 32 274 32 569
Fragments arranged by size:
578 569 274 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 297 372 1485
Size: 297 75 1113
Fragments arranged by size:
1113 297 75
XhoI C'TCGA_G
Cuts at: 0 1024 1485
Size: 1024 461
XmaI C'CCGG_G
Cuts at: 0 101 107 1423 1485
Size: 101 6 1316 62
Fragments arranged by size:
1316 101 62 6
Enzymes that do cut:
AccI AfeI AflIII AlfI AloI AlwNI ApaI ApoI
AscI AvaI AvrII BanI BanII BbeI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BsrDI BsrFI BssHII BssSI BstAPI
BstXI BtgI BtsI CspCI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII KasI MscI
MspA1I NaeI NarI NcoI NgoMIV NotI PflMI PfoI
PmlI PpiI PspOMI PspXI PsrI PstI PvuII SacI
SacII SfcI SfoI SmaI SmlI StyI TatI TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI ApaLI AseI AsiSI BaeI BamHI BbsI BcgI
BciVI BclI BfrBI BglII BmtI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrGI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MslI NdeI NheI
NruI NsiI NspI PacI PasI PciI PmeI PpuMI
PshAI PsiI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XmnI
ZraI