Restriction map for
pFN21AE4471
(Linear) (Six-base) MAPSORT of: KIEE4471.seq Check: 5250 from: 1 to: 1476
KIEE4471.orf
With 182 enzymes: SgfI *
March 22, 2012 18:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 640 671 1476
Size: 293 347 31 805
Fragments arranged by size:
805 347 293 31
AleI CACnn'nnGTG
Cuts at: 0 940 1312 1476
Size: 940 372 164
Fragments arranged by size:
940 372 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1135 1476
Size: 1135 341
AlwNI CAG_nnn'CTG
Cuts at: 0 271 322 1476
Size: 271 51 1154
Fragments arranged by size:
1154 271 51
ApoI r'AATT_y
Cuts at: 0 382 978 1063 1234 1370 1476
Size: 382 596 85 171 136 106
Fragments arranged by size:
596 382 171 136 106 85
AseI AT'TA_AT
Cuts at: 0 59 1476
Size: 59 1417
AvaI C'yCGr_G
Cuts at: 0 109 1476
Size: 109 1367
BanI G'GyrC_C
Cuts at: 0 1119 1476
Size: 1119 357
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 286 1476
Size: 211 75 1190
Fragments arranged by size:
1190 211 75
BfrBI ATG'CAT
Cuts at: 0 579 1476
Size: 579 897
BglII A'GATC_T
Cuts at: 0 434 1476
Size: 434 1042
BmrI ACTGGGnnnn_n'
Cuts at: 0 1259 1476
Size: 1259 217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1476
Size: 1153 323
Bpu10I CC'TnA_GC
Cuts at: 0 149 1476
Size: 149 1327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 981 1476
Size: 796 185 495
Fragments arranged by size:
796 495 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 1327 1476
Size: 1327 149
BsaAI yAC'GTr
Cuts at: 0 294 1314 1476
Size: 294 1020 162
Fragments arranged by size:
1020 294 162
BsaWI w'CCGG_w
Cuts at: 0 444 764 1476
Size: 444 320 712
Fragments arranged by size:
712 444 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 251 281 770 800 1476
Size: 251 30 489 30 676
Fragments arranged by size:
676 489 251 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1263 1476
Size: 1263 213
BseYI C'CCAG_C
Cuts at: 0 1161 1476
Size: 1161 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1336 1476
Size: 1037 299 140
Fragments arranged by size:
1037 299 140
BsiHKAI G_wGCw'C
Cuts at: 0 1397 1476
Size: 1397 79
Bsp1286I G_dGCh'C
Cuts at: 0 1397 1476
Size: 1397 79
BspHI T'CATG_A
Cuts at: 0 791 860 1476
Size: 791 69 616
Fragments arranged by size:
791 616 69
BsrDI GCAATG_nn'
Cuts at: 0 1289 1476
Size: 1289 187
BsrFI r'CCGG_y
Cuts at: 0 239 1476
Size: 239 1237
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 582 748 1218 1476
Size: 210 372 166 470 258
Fragments arranged by size:
470 372 258 210 166
BstYI r'GATC_y
Cuts at: 0 401 434 1476
Size: 401 33 1042
Fragments arranged by size:
1042 401 33
BtgI C'CryG_G
Cuts at: 0 596 689 1010 1476
Size: 596 93 321 466
Fragments arranged by size:
596 466 321 93
BtsI GCAGTG_nn'
Cuts at: 0 272 1476
Size: 272 1204
EaeI y'GGCC_r
Cuts at: 0 1007 1220 1476
Size: 1007 213 256
Fragments arranged by size:
1007 256 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 640 671 1153 1476
Size: 293 347 31 482 323
Fragments arranged by size:
482 347 323 293 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1222 1476
Size: 1222 254
EcoO109I rG'GnC_Cy
Cuts at: 0 1128 1476
Size: 1128 348
HaeII r_GCGC'y
Cuts at: 0 1259 1476
Size: 1259 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 770 802 1191 1223 1476
Size: 770 32 389 32 253
Fragments arranged by size:
770 389 253 32 32
HincII GTy'rAC
Cuts at: 0 554 1077 1365 1437 1476
Size: 554 523 288 72 39
Fragments arranged by size:
554 523 288 72 39
HpaI GTT'AAC
Cuts at: 0 554 1476
Size: 554 922
MscI TGG'CCA
Cuts at: 0 1222 1476
Size: 1222 254
MslI CAynn'nnrTG
Cuts at: 0 292 325 601 940 1312 1476
Size: 292 33 276 339 372 164
Fragments arranged by size:
372 339 292 276 164 33
MspA1I CmG'CkG
Cuts at: 0 649 1476
Size: 649 827
NcoI C'CATG_G
Cuts at: 0 596 1010 1476
Size: 596 414 466
Fragments arranged by size:
596 466 414
NdeI CA'TA_TG
Cuts at: 0 999 1431 1476
Size: 999 432 45
Fragments arranged by size:
999 432 45
NsiI A_TGCA'T
Cuts at: 0 581 1476
Size: 581 895
NspI r_CATG'y
Cuts at: 0 157 610 1476
Size: 157 453 866
Fragments arranged by size:
866 453 157
PflMI CCAn_nnn'nTGG
Cuts at: 0 1016 1476
Size: 1016 460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 893 925 1476
Size: 893 32 551
Fragments arranged by size:
893 551 32
PvuII CAG'CTG
Cuts at: 0 649 1476
Size: 649 827
SmaI CCC'GGG
Cuts at: 0 111 1476
Size: 111 1365
SmlI C'TyrA_G
Cuts at: 0 811 960 1476
Size: 811 149 516
Fragments arranged by size:
811 516 149
SphI G_CATG'C
Cuts at: 0 157 610 1476
Size: 157 453 866
Fragments arranged by size:
866 453 157
SspI AAT'ATT
Cuts at: 0 63 1476
Size: 63 1413
StyI C'CwwG_G
Cuts at: 0 39 596 1010 1223 1250 1301 1476
Size: 39 557 414 213 27 51 175
Fragments arranged by size:
557 414 213 175 51 39 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1314 1476
Size: 1314 162
TatI w'GTAC_w
Cuts at: 0 332 772 1476
Size: 332 440 704
Fragments arranged by size:
704 440 332
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 251 283 1476
Size: 251 32 1193
Fragments arranged by size:
1193 251 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1410 1476
Size: 1410 66
XmaI C'CCGG_G
Cuts at: 0 109 1476
Size: 109 1367
XmnI GAAnn'nnTTC
Cuts at: 0 590 1476
Size: 590 886
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApoI AseI AvaI BanI
BbsI BfrBI BglII BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrDI BsrFI BstXI BstYI BtgI BtsI EaeI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HpaI MscI
MslI MspA1I NcoI NdeI NsiI NspI PflMI PpiI
PvuII SmaI SmlI SphI SspI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI
BglI BlpI Bme1580I BmgBI BmtI BplI BsaBI BsaHI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII
KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI Tth111I XbaI XhoI ZraI