Restriction map for
pFN21AE4468
(Linear) (Six-base) MAPSORT of: KIEE4468.seq Check: 3813 from: 1 to: 1470
KIEE4468.orf
With 182 enzymes: SgfI *
March 22, 2012 18:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 961 1470
Size: 232 729 509
Fragments arranged by size:
729 509 232
AlwNI CAG_nnn'CTG
Cuts at: 0 307 1048 1470
Size: 307 741 422
Fragments arranged by size:
741 422 307
ApaLI G'TGCA_C
Cuts at: 0 1054 1470
Size: 1054 416
ApoI r'AATT_y
Cuts at: 0 396 598 705 841 1123 1198 1470
Size: 396 202 107 136 282 75 272
Fragments arranged by size:
396 282 272 202 136 107 75
AvaI C'yCGr_G
Cuts at: 0 779 1470
Size: 779 691
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1429 1462 1470
Size: 1429 33 8
Fragments arranged by size:
1429 33 8
BamHI G'GATC_C
Cuts at: 0 3 635 1470
Size: 3 632 835
Fragments arranged by size:
835 632 3
BanII G_rGCy'C
Cuts at: 0 70 628 1009 1470
Size: 70 558 381 461
Fragments arranged by size:
558 461 381 70
BclI T'GATC_A
Cuts at: 0 264 803 1470
Size: 264 539 667
Fragments arranged by size:
667 539 264
BfrBI ATG'CAT
Cuts at: 0 1423 1470
Size: 1423 47
Bme1580I G_kGCm'C
Cuts at: 0 1058 1153 1470
Size: 1058 95 317
Fragments arranged by size:
1058 317 95
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 100 929 1470
Size: 100 829 541
Fragments arranged by size:
829 541 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1470
Size: 78 1392
BsaWI w'CCGG_w
Cuts at: 0 1001 1470
Size: 1001 469
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 917 947 996 1026 1470
Size: 917 30 49 30 444
Fragments arranged by size:
917 444 49 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 92 289 429 475 1072 1470
Size: 92 197 140 46 597 398
Fragments arranged by size:
597 398 197 140 92 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1470
Size: 1168 302
BsiHKAI G_wGCw'C
Cuts at: 0 26 70 519 628 780 910 1058 1470
Size: 26 44 449 109 152 130 148 412
Fragments arranged by size:
449 412 152 148 130 109 44 26
Bsp1286I G_dGCh'C
Cuts at: 0 26 70 519 628 780 910 1009 1058
Size: 26 44 449 109 152 130 99 49
Cuts at: 1058 1153 1470
Size: 95 317
Fragments arranged by size:
449 317 152 130 109 99 95 49
44 26
BspHI T'CATG_A
Cuts at: 0 383 1470
Size: 383 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1103 1470
Size: 1103 367
BsrDI GCAATG_nn'
Cuts at: 0 351 531 1412 1470
Size: 351 180 881 58
Fragments arranged by size:
881 351 180 58
BssSI C'ACGA_G
Cuts at: 0 1057 1470
Size: 1057 413
BstYI r'GATC_y
Cuts at: 0 3 365 635 1061 1470
Size: 3 362 270 426 409
Fragments arranged by size:
426 409 362 270 3
Bsu36I CC'TnA_GG
Cuts at: 0 613 1470
Size: 613 857
BtgI C'CryG_G
Cuts at: 0 631 1470
Size: 631 839
BtsI GCAGTG_nn'
Cuts at: 0 251 1112 1470
Size: 251 861 358
Fragments arranged by size:
861 358 251
ClaI AT'CG_AT
Cuts at: 0 555 668 1470
Size: 555 113 802
Fragments arranged by size:
802 555 113
DraIII CAC_nnn'GTG
Cuts at: 0 490 1470
Size: 490 980
EaeI y'GGCC_r
Cuts at: 0 1227 1470
Size: 1227 243
EarI CTCTTCn'nnn_
Cuts at: 0 69 282 406 968 1470
Size: 69 213 124 562 502
Fragments arranged by size:
562 502 213 124 69
EcoICRI GAG'CTC
Cuts at: 0 68 626 1470
Size: 68 558 844
Fragments arranged by size:
844 558 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 232 961 1470
Size: 78 154 729 509
Fragments arranged by size:
729 509 154 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 1470
Size: 457 1013
EcoO109I rG'GnC_Cy
Cuts at: 0 255 1314 1470
Size: 255 1059 156
Fragments arranged by size:
1059 255 156
EcoRI G'AATT_C
Cuts at: 0 1198 1470
Size: 1198 272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 317 349 911 917 943 949 1470
Size: 317 32 562 6 26 6 521
Fragments arranged by size:
562 521 317 32 26 6 6
HincII GTy'rAC
Cuts at: 0 318 1386 1470
Size: 318 1068 84
Fragments arranged by size:
1068 318 84
HindIII A'AGCT_T
Cuts at: 0 855 1470
Size: 855 615
HpaI GTT'AAC
Cuts at: 0 318 1470
Size: 318 1152
MscI TGG'CCA
Cuts at: 0 1229 1470
Size: 1229 241
MslI CAynn'nnrTG
Cuts at: 0 223 236 1470
Size: 223 13 1234
Fragments arranged by size:
1234 223 13
MspA1I CmG'CkG
Cuts at: 0 10 449 1470
Size: 10 439 1021
Fragments arranged by size:
1021 439 10
NcoI C'CATG_G
Cuts at: 0 631 1470
Size: 631 839
NsiI A_TGCA'T
Cuts at: 0 1425 1470
Size: 1425 45
NspI r_CATG'y
Cuts at: 0 1035 1470
Size: 1035 435
PflMI CCAn_nnn'nTGG
Cuts at: 0 1236 1470
Size: 1236 234
PfoI T'CCnGG_A
Cuts at: 0 968 1470
Size: 968 502
PshAI GACnn'nnGTC
Cuts at: 0 1025 1470
Size: 1025 445
PsiI TTA'TAA
Cuts at: 0 566 1470
Size: 566 904
PstI C_TGCA'G
Cuts at: 0 131 1470
Size: 131 1339
PvuII CAG'CTG
Cuts at: 0 10 1470
Size: 10 1460
SacI G_AGCT'C
Cuts at: 0 70 628 1470
Size: 70 558 842
Fragments arranged by size:
842 558 70
SfcI C'TryA_G
Cuts at: 0 127 1470
Size: 127 1343
StyI C'CwwG_G
Cuts at: 0 631 1145 1470
Size: 631 514 325
Fragments arranged by size:
631 514 325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 1470
Size: 504 966
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI AvaI BaeI BamHI BanII
BclI BfrBI Bme1580I BplI BpmI BsaWI BsaXI BseRI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BssSI BstYI
Bsu36I BtgI BtsI ClaI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII HpaI
MscI MslI MspA1I NcoI NsiI NspI PflMI PfoI
PshAI PsiI PstI PvuII SacI SfcI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvrII BanI BbeI BbsI BbvCI BcgI BciVI
BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgZI CspCI DraI DrdI EagI
EciI EcoRV FalI FseI FspI FspAI HaeII KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI