Restriction map for
pFN21AE4467
(Linear) (Six-base) MAPSORT of: KIEE4467.seq Check: 9541 from: 1 to: 1467
KIEE4467.orf
With 182 enzymes: SgfI *
March 22, 2012 18:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 21 1467
Size: 21 1446
AccI GT'mk_AC
Cuts at: 0 854 911 1467
Size: 854 57 556
Fragments arranged by size:
854 556 57
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 628 1467
Size: 567 61 839
Fragments arranged by size:
839 567 61
AflIII A'CryG_T
Cuts at: 0 1208 1467
Size: 1208 259
AhdI GACnn_n'nnGTC
Cuts at: 0 1359 1467
Size: 1359 108
AleI CACnn'nnGTG
Cuts at: 0 208 280 1467
Size: 208 72 1187
Fragments arranged by size:
1187 208 72
AlwNI CAG_nnn'CTG
Cuts at: 0 519 931 1085 1467
Size: 519 412 154 382
Fragments arranged by size:
519 412 382 154
ApaI G_GGCC'C
Cuts at: 0 164 1467
Size: 164 1303
ApaLI G'TGCA_C
Cuts at: 0 862 1467
Size: 862 605
AvaI C'yCGr_G
Cuts at: 0 58 1074 1467
Size: 58 1016 393
Fragments arranged by size:
1016 393 58
BanI G'GyrC_C
Cuts at: 0 3 33 77 240 949 1467
Size: 3 30 44 163 709 518
Fragments arranged by size:
709 518 163 44 30 3
BanII G_rGCy'C
Cuts at: 0 65 164 302 371 1184 1467
Size: 65 99 138 69 813 283
Fragments arranged by size:
813 283 138 99 69 65
BbeI G_GCGC'C
Cuts at: 0 81 953 1467
Size: 81 872 514
Fragments arranged by size:
872 514 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 816 1041 1188 1467
Size: 816 225 147 279
Fragments arranged by size:
816 279 225 147
BglI GCCn_nnn'nGGC
Cuts at: 0 1036 1467
Size: 1036 431
BlpI GC'TnA_GC
Cuts at: 0 301 1467
Size: 301 1166
Bme1580I G_kGCm'C
Cuts at: 0 8 38 164 245 866 1142 1191 1467
Size: 8 30 126 81 621 276 49 276
Fragments arranged by size:
621 276 276 126 81 49 30 8
BmgBI CAC'GTC
Cuts at: 0 959 1260 1467
Size: 959 301 207
Fragments arranged by size:
959 301 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1467
Size: 700 767
BsaAI yAC'GTr
Cuts at: 0 861 1467
Size: 861 606
BsaHI Gr'CG_yC
Cuts at: 0 78 950 1022 1467
Size: 78 872 72 445
Fragments arranged by size:
872 445 78 72
BsaWI w'CCGG_w
Cuts at: 0 65 1467
Size: 65 1402
BseYI C'CCAG_C
Cuts at: 0 15 333 483 507 711 771 945 989
Size: 15 318 150 24 204 60 174 44
Cuts at: 989 1467
Size: 478
Fragments arranged by size:
478 318 204 174 150 60 44 24
15
BsiEI CG_ry'CG
Cuts at: 0 648 793 836 1467
Size: 648 145 43 631
Fragments arranged by size:
648 631 145 43
BsiHKAI G_wGCw'C
Cuts at: 0 65 371 866 1184 1467
Size: 65 306 495 318 283
Fragments arranged by size:
495 318 306 283 65
Bsp1286I G_dGCh'C
Cuts at: 0 8 38 65 164 245 302 371 866
Size: 8 30 27 99 81 57 69 495
Cuts at: 866 1142 1184 1191 1467
Size: 276 42 7 276
Fragments arranged by size:
495 276 276 99 81 69 57 42
30 27 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 21 177 275 1467
Size: 21 156 98 1192
Fragments arranged by size:
1192 156 98 21
BsrFI r'CCGG_y
Cuts at: 0 207 279 457 835 1467
Size: 207 72 178 378 632
Fragments arranged by size:
632 378 207 178 72
BssHII G'CGCG_C
Cuts at: 0 394 439 468 994 1467
Size: 394 45 29 526 473
Fragments arranged by size:
526 473 394 45 29
BssSI C'ACGA_G
Cuts at: 0 82 427 1135 1467
Size: 82 345 708 332
Fragments arranged by size:
708 345 332 82
BstAPI GCAn_nnn'nTGC
Cuts at: 0 931 1467
Size: 931 536
BstXI CCAn_nnnn'nTGG
Cuts at: 0 945 1467
Size: 945 522
BstYI r'GATC_y
Cuts at: 0 983 1467
Size: 983 484
Bsu36I CC'TnA_GG
Cuts at: 0 1417 1467
Size: 1417 50
BtgI C'CryG_G
Cuts at: 0 38 386 968 1116 1431 1467
Size: 38 348 582 148 315 36
Fragments arranged by size:
582 348 315 148 38 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 384 999 1128 1467
Size: 384 615 129 339
Fragments arranged by size:
615 384 339 129
DraIII CAC_nnn'GTG
Cuts at: 0 345 1467
Size: 345 1122
EaeI y'GGCC_r
Cuts at: 0 124 230 389 455 790 833 1028 1467
Size: 124 106 159 66 335 43 195 439
Fragments arranged by size:
439 335 195 159 124 106 66 43
EagI C'GGCC_G
Cuts at: 0 790 833 1467
Size: 790 43 634
Fragments arranged by size:
790 634 43
EarI CTCTTCn'nnn_
Cuts at: 0 376 607 634 1467
Size: 376 231 27 833
Fragments arranged by size:
833 376 231 27
EcoICRI GAG'CTC
Cuts at: 0 63 369 1182 1467
Size: 63 306 813 285
Fragments arranged by size:
813 306 285 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 628 700 1467
Size: 567 61 72 767
Fragments arranged by size:
767 567 72 61
EcoO109I rG'GnC_Cy
Cuts at: 0 160 1266 1467
Size: 160 1106 201
Fragments arranged by size:
1106 201 160
HaeII r_GCGC'y
Cuts at: 0 81 615 953 1467
Size: 81 534 338 514
Fragments arranged by size:
534 514 338 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 919 951 1345 1377 1467
Size: 52 32 835 32 394 32 90
Fragments arranged by size:
835 394 90 52 32 32 32
HincII GTy'rAC
Cuts at: 0 1206 1467
Size: 1206 261
KasI G'GCGC_C
Cuts at: 0 77 949 1467
Size: 77 872 518
Fragments arranged by size:
872 518 77
MslI CAynn'nnrTG
Cuts at: 0 208 280 1467
Size: 208 72 1187
Fragments arranged by size:
1187 208 72
MspA1I CmG'CkG
Cuts at: 0 19 516 798 970 989 1118 1379 1467
Size: 19 497 282 172 19 129 261 88
Fragments arranged by size:
497 282 261 172 129 88 19 19
NaeI GCC'GGC
Cuts at: 0 459 837 1467
Size: 459 378 630
Fragments arranged by size:
630 459 378
NarI GG'CG_CC
Cuts at: 0 78 950 1467
Size: 78 872 517
Fragments arranged by size:
872 517 78
NcoI C'CATG_G
Cuts at: 0 386 1431 1467
Size: 386 1045 36
Fragments arranged by size:
1045 386 36
NgoMIV G'CCGG_C
Cuts at: 0 457 835 1467
Size: 457 378 632
Fragments arranged by size:
632 457 378
NotI GC'GGCC_GC
Cuts at: 0 790 1467
Size: 790 677
NspI r_CATG'y
Cuts at: 0 357 1309 1467
Size: 357 952 158
Fragments arranged by size:
952 357 158
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 453 519 1467
Size: 345 108 66 948
Fragments arranged by size:
948 345 108 66
PspOMI G'GGCC_C
Cuts at: 0 160 1467
Size: 160 1307
PstI C_TGCA'G
Cuts at: 0 296 1467
Size: 296 1171
PvuII CAG'CTG
Cuts at: 0 516 798 1467
Size: 516 282 669
Fragments arranged by size:
669 516 282
RsrII CG'GwC_CG
Cuts at: 0 1281 1467
Size: 1281 186
SacI G_AGCT'C
Cuts at: 0 65 371 1184 1467
Size: 65 306 813 283
Fragments arranged by size:
813 306 283 65
SacII CC_GC'GG
Cuts at: 0 971 1119 1467
Size: 971 148 348
Fragments arranged by size:
971 348 148
SapI GCTCTTCn'nnn_
Cuts at: 0 376 1467
Size: 376 1091
SexAI A'CCwGG_T
Cuts at: 0 845 1467
Size: 845 622
SfcI C'TryA_G
Cuts at: 0 292 375 1467
Size: 292 83 1092
Fragments arranged by size:
1092 292 83
SfoI GGC'GCC
Cuts at: 0 79 951 1467
Size: 79 872 516
Fragments arranged by size:
872 516 79
SgrAI Cr'CCGG_yG
Cuts at: 0 207 279 1467
Size: 207 72 1188
Fragments arranged by size:
1188 207 72
StyI C'CwwG_G
Cuts at: 0 386 1431 1467
Size: 386 1045 36
Fragments arranged by size:
1045 386 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 504 1467
Size: 18 486 963
Fragments arranged by size:
963 486 18
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BglI BlpI
Bme1580I BmgBI BpmI BsaAI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PflMI
PspOMI PstI PvuII RsrII SacI SacII SapI SexAI
SfcI SfoI SgrAI StyI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstBI
BstEII BstZ17I BtsI ClaI CspCI DraI DrdI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI MscI NdeI NheI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI SalI
SanDI SbfI ScaI SfiI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI