Restriction map for
pFN21AE4465
(Linear) (Six-base) MAPSORT of: KIEE4465.seq Check: 2134 from: 1 to: 1461
KIEE4465.orf
With 182 enzymes: SgfI *
March 22, 2012 18:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1402 1461
Size: 1402 59
AflIII A'CryG_T
Cuts at: 0 812 1461
Size: 812 649
AhdI GACnn_n'nnGTC
Cuts at: 0 71 1461
Size: 71 1390
ApaI G_GGCC'C
Cuts at: 0 191 317 1461
Size: 191 126 1144
Fragments arranged by size:
1144 191 126
ApoI r'AATT_y
Cuts at: 0 615 1461
Size: 615 846
BamHI G'GATC_C
Cuts at: 0 776 1461
Size: 776 685
BanI G'GyrC_C
Cuts at: 0 121 235 637 1040 1224 1461
Size: 121 114 402 403 184 237
Fragments arranged by size:
403 402 237 184 121 114
BanII G_rGCy'C
Cuts at: 0 191 317 1386 1461
Size: 191 126 1069 75
Fragments arranged by size:
1069 191 126 75
BbeI G_GCGC'C
Cuts at: 0 641 1044 1461
Size: 641 403 417
Fragments arranged by size:
641 417 403
BbsI GAAGACnn'nnnn_
Cuts at: 0 1457 1461
Size: 1457 4
BbvCI CC'TCA_GC
Cuts at: 0 388 1461
Size: 388 1073
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1392 1426 1461
Size: 1392 34 35
Fragments arranged by size:
1392 35 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 299 1461
Size: 299 1162
BclI T'GATC_A
Cuts at: 0 266 1461
Size: 266 1195
BlpI GC'TnA_GC
Cuts at: 0 166 1461
Size: 166 1295
Bme1580I G_kGCm'C
Cuts at: 0 124 191 240 317 1229 1461
Size: 124 67 49 77 912 232
Fragments arranged by size:
912 232 124 77 67 49
BmgBI CAC'GTC
Cuts at: 0 368 1461
Size: 368 1093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1461
Size: 1034 427
Bpu10I CC'TnA_GC
Cuts at: 0 388 1461
Size: 388 1073
BsaAI yAC'GTr
Cuts at: 0 987 1461
Size: 987 474
BsaBI GATnn'nnATC
Cuts at: 0 1093 1461
Size: 1093 368
BsaHI Gr'CG_yC
Cuts at: 0 638 1041 1461
Size: 638 403 420
Fragments arranged by size:
638 420 403
BsaWI w'CCGG_w
Cuts at: 0 1443 1461
Size: 1443 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 349 1461
Size: 319 30 1112
Fragments arranged by size:
1112 319 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 428 1461
Size: 428 1033
BseYI C'CCAG_C
Cuts at: 0 87 231 513 641 1461
Size: 87 144 282 128 820
Fragments arranged by size:
820 282 144 128 87
BsiHKAI G_wGCw'C
Cuts at: 0 167 593 1461
Size: 167 426 868
Fragments arranged by size:
868 426 167
Bsp1286I G_dGCh'C
Cuts at: 0 124 167 191 240 317 593 1229 1386
Size: 124 43 24 49 77 276 636 157
Cuts at: 1386 1461
Size: 75
Fragments arranged by size:
636 276 157 124 77 75 49 43
24
BspHI T'CATG_A
Cuts at: 0 806 1461
Size: 806 655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 761 967 1461
Size: 761 206 494
Fragments arranged by size:
761 494 206
BstEII G'GTnAC_C
Cuts at: 0 880 1461
Size: 880 581
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 666 1461
Size: 117 549 795
Fragments arranged by size:
795 549 117
BstYI r'GATC_y
Cuts at: 0 140 776 1461
Size: 140 636 685
Fragments arranged by size:
685 636 140
BtgI C'CryG_G
Cuts at: 0 542 1461
Size: 542 919
EaeI y'GGCC_r
Cuts at: 0 461 545 563 761 1461
Size: 461 84 18 198 700
Fragments arranged by size:
700 461 198 84 18
EarI CTCTTCn'nnn_
Cuts at: 0 337 1461
Size: 337 1124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1402 1461
Size: 1034 368 59
Fragments arranged by size:
1034 368 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 1461
Size: 301 1160
EcoO109I rG'GnC_Cy
Cuts at: 0 187 188 219 956 1461
Size: 187 1 31 737 505
Fragments arranged by size:
737 505 187 31 1
HaeII r_GCGC'y
Cuts at: 0 641 1044 1461
Size: 641 403 417
Fragments arranged by size:
641 417 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 89 1099 1131 1461
Size: 25 32 32 1010 32 330
Fragments arranged by size:
1010 330 32 32 32 25
KasI G'GCGC_C
Cuts at: 0 637 1040 1461
Size: 637 403 421
Fragments arranged by size:
637 421 403
MscI TGG'CCA
Cuts at: 0 565 763 1461
Size: 565 198 698
Fragments arranged by size:
698 565 198
MslI CAynn'nnrTG
Cuts at: 0 115 805 811 829 985 1183 1261 1461
Size: 115 690 6 18 156 198 78 200
Fragments arranged by size:
690 200 198 156 115 78 18 6
MspA1I CmG'CkG
Cuts at: 0 544 581 645 970 1461
Size: 544 37 64 325 491
Fragments arranged by size:
544 491 325 64 37
NarI GG'CG_CC
Cuts at: 0 638 1041 1461
Size: 638 403 420
Fragments arranged by size:
638 420 403
NdeI CA'TA_TG
Cuts at: 0 202 1461
Size: 202 1259
NruI TCG'CGA
Cuts at: 0 226 1461
Size: 226 1235
NspI r_CATG'y
Cuts at: 0 1011 1461
Size: 1011 450
PflMI CCAn_nnn'nTGG
Cuts at: 0 864 1461
Size: 864 597
PpuMI rG'GwC_Cy
Cuts at: 0 219 956 1461
Size: 219 737 505
Fragments arranged by size:
737 505 219
PspOMI G'GGCC_C
Cuts at: 0 187 313 1461
Size: 187 126 1148
Fragments arranged by size:
1148 187 126
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 802 834 1461
Size: 802 32 627
Fragments arranged by size:
802 627 32
PstI C_TGCA'G
Cuts at: 0 854 1461
Size: 854 607
PvuII CAG'CTG
Cuts at: 0 645 1461
Size: 645 816
SacII CC_GC'GG
Cuts at: 0 545 1461
Size: 545 916
SfcI C'TryA_G
Cuts at: 0 850 1461
Size: 850 611
SfoI GGC'GCC
Cuts at: 0 639 1042 1461
Size: 639 403 419
Fragments arranged by size:
639 419 403
StuI AGG'CCT
Cuts at: 0 307 1215 1461
Size: 307 908 246
Fragments arranged by size:
908 307 246
StyI C'CwwG_G
Cuts at: 0 600 1461
Size: 600 861
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 223 1461
Size: 223 1238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1166 1198 1461
Size: 1166 32 263
Fragments arranged by size:
1166 263 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1372 1461
Size: 1372 89
XmnI GAAnn'nnTTC
Cuts at: 0 535 1461
Size: 535 926
Enzymes that do cut:
AcuI AflIII AhdI ApaI ApoI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BlpI Bme1580I
BmgBI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BstEII BstXI
BstYI BtgI EaeI EarI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MscI MslI MspA1I NarI NdeI NruI
NspI PflMI PpuMI PspOMI PsrI PstI PvuII SacII
SfcI SfoI StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BfrBI BglI BglII BmrI BmtI
BplI BpuEI BsaI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NcoI NgoMIV NheI NotI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmaI ZraI