Restriction map for
pFN21AE4463
(Linear) (Six-base) MAPSORT of: KIEE4463.seq Check: 3499 from: 1 to: 1440
KIEE4463.orf
With 182 enzymes: SgfI *
March 22, 2012 18:06 ..
AccI GT'mk_AC
Cuts at: 0 86 1440
Size: 86 1354
Acc65I G'GTAC_C
Cuts at: 0 107 1440
Size: 107 1333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1440
Size: 1018 422
AflIII A'CryG_T
Cuts at: 0 152 1440
Size: 152 1288
AhdI GACnn_n'nnGTC
Cuts at: 0 82 1440
Size: 82 1358
AleI CACnn'nnGTG
Cuts at: 0 457 1440
Size: 457 983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 827 855 1440
Size: 827 28 585
Fragments arranged by size:
827 585 28
ApoI r'AATT_y
Cuts at: 0 13 921 1440
Size: 13 908 519
Fragments arranged by size:
908 519 13
AvaI C'yCGr_G
Cuts at: 0 103 364 1440
Size: 103 261 1076
Fragments arranged by size:
1076 261 103
BamHI G'GATC_C
Cuts at: 0 1182 1440
Size: 1182 258
BanI G'GyrC_C
Cuts at: 0 107 854 1440
Size: 107 747 586
Fragments arranged by size:
747 586 107
BanII G_rGCy'C
Cuts at: 0 104 521 1440
Size: 104 417 919
Fragments arranged by size:
919 417 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 797 1440
Size: 797 643
BglI GCCn_nnn'nGGC
Cuts at: 0 469 1440
Size: 469 971
BmgBI CAC'GTC
Cuts at: 0 96 1419 1440
Size: 96 1323 21
Fragments arranged by size:
1323 96 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 1066 1440
Size: 1066 374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 431 697 1440
Size: 361 70 266 743
Fragments arranged by size:
743 361 266 70
BsaAI yAC'GTr
Cuts at: 0 48 1440
Size: 48 1392
BsaHI Gr'CG_yC
Cuts at: 0 78 1440
Size: 78 1362
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 1440
Size: 91 30 1319
Fragments arranged by size:
1319 91 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 437 567 1440
Size: 437 130 873
Fragments arranged by size:
873 437 130
BseYI C'CCAG_C
Cuts at: 0 324 592 1440
Size: 324 268 848
Fragments arranged by size:
848 324 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 1399 1440
Size: 787 612 41
Fragments arranged by size:
787 612 41
BsiEI CG_ry'CG
Cuts at: 0 118 1415 1440
Size: 118 1297 25
Fragments arranged by size:
1297 118 25
BsiHKAI G_wGCw'C
Cuts at: 0 521 1440
Size: 521 919
BsmI GAATG_Cn'
Cuts at: 0 832 1440
Size: 832 608
Bsp1286I G_dGCh'C
Cuts at: 0 104 521 1440
Size: 104 417 919
Fragments arranged by size:
919 417 104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 1440
Size: 119 1321
BsrBI CCG'CTC
Cuts at: 0 475 1440
Size: 475 965
BsrFI r'CCGG_y
Cuts at: 0 1037 1440
Size: 1037 403
BstAPI GCAn_nnn'nTGC
Cuts at: 0 829 1440
Size: 829 611
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 459 1440
Size: 60 399 981
Fragments arranged by size:
981 399 60
BstYI r'GATC_y
Cuts at: 0 1182 1440
Size: 1182 258
Bsu36I CC'TnA_GG
Cuts at: 0 140 1318 1440
Size: 140 1178 122
Fragments arranged by size:
1178 140 122
BtsI GCAGTG_nn'
Cuts at: 0 242 834 1440
Size: 242 592 606
Fragments arranged by size:
606 592 242
DrdI GACnn_nn'nnGTC
Cuts at: 0 1330 1440
Size: 1330 110
EaeI y'GGCC_r
Cuts at: 0 50 115 260 470 1412 1440
Size: 50 65 145 210 942 28
Fragments arranged by size:
942 210 145 65 50 28
EagI C'GGCC_G
Cuts at: 0 115 1412 1440
Size: 115 1297 28
Fragments arranged by size:
1297 115 28
EarI CTCTTCn'nnn_
Cuts at: 0 1271 1440
Size: 1271 169
EcoICRI GAG'CTC
Cuts at: 0 519 1440
Size: 519 921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 431 697 1018 1440
Size: 361 70 266 321 422
Fragments arranged by size:
422 361 321 266 70
EcoRI G'AATT_C
Cuts at: 0 13 1440
Size: 13 1427
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 413 445 1150 1182 1440
Size: 68 32 313 32 705 32 258
Fragments arranged by size:
705 313 258 68 32 32 32
KpnI G_GTAC'C
Cuts at: 0 111 1440
Size: 111 1329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1440
Size: 1271 169
MscI TGG'CCA
Cuts at: 0 52 1440
Size: 52 1388
MslI CAynn'nnrTG
Cuts at: 0 457 809 943 1440
Size: 457 352 134 497
Fragments arranged by size:
497 457 352 134
MspA1I CmG'CkG
Cuts at: 0 120 133 1149 1383 1440
Size: 120 13 1016 234 57
Fragments arranged by size:
1016 234 120 57 13
NdeI CA'TA_TG
Cuts at: 0 796 1440
Size: 796 644
NotI GC'GGCC_GC
Cuts at: 0 115 1440
Size: 115 1325
NspI r_CATG'y
Cuts at: 0 279 332 743 1144 1440
Size: 279 53 411 401 296
Fragments arranged by size:
411 401 296 279 53
PasI CC'CwG_GG
Cuts at: 0 726 1440
Size: 726 714
PstI C_TGCA'G
Cuts at: 0 503 668 1124 1440
Size: 503 165 456 316
Fragments arranged by size:
503 456 316 165
PvuII CAG'CTG
Cuts at: 0 1149 1440
Size: 1149 291
SacI G_AGCT'C
Cuts at: 0 521 1440
Size: 521 919
SapI GCTCTTCn'nnn_
Cuts at: 0 1271 1440
Size: 1271 169
SbfI CC_TGCA'GG
Cuts at: 0 668 1440
Size: 668 772
SfcI C'TryA_G
Cuts at: 0 499 664 1120 1440
Size: 499 165 456 320
Fragments arranged by size:
499 456 320 165
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 469 1440
Size: 469 971
SmaI CCC'GGG
Cuts at: 0 105 1440
Size: 105 1335
SphI G_CATG'C
Cuts at: 0 332 1440
Size: 332 1108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 760 933 1440
Size: 760 173 507
Fragments arranged by size:
760 507 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 1440
Size: 265 32 1143
Fragments arranged by size:
1143 265 32
XmaI C'CCGG_G
Cuts at: 0 103 1440
Size: 103 1337
XmnI GAAnn'nnTTC
Cuts at: 0 1211 1265 1440
Size: 1211 54 175
Fragments arranged by size:
1211 175 54
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AleI AlfI ApoI
AvaI BamHI BanI BanII BbsI BglI BmgBI BmrI
BpmI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
BstYI Bsu36I BtsI DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoRI Hin4I KpnI MmeI MscI MslI MspA1I
NdeI NotI NspI PasI PstI PvuII SacI SapI
SbfI SfcI SfiI SmaI SphI TaqII TstI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
EciI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI StyI SwaI TatI Tth111I XbaI
XcmI XhoI ZraI