Restriction map for
pFN21AE4460
(Linear) (Six-base) MAPSORT of: KIEE4460.seq Check: 1032 from: 1 to: 1431
KIEE4460.orf
With 182 enzymes: SgfI *
March 22, 2012 18:06 ..
Acc65I G'GTAC_C
Cuts at: 0 822 1431
Size: 822 609
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 716 995 1301 1431
Size: 282 434 279 306 130
Fragments arranged by size:
434 306 282 279 130
AlwNI CAG_nnn'CTG
Cuts at: 0 850 1431
Size: 850 581
ApoI r'AATT_y
Cuts at: 0 1245 1327 1431
Size: 1245 82 104
Fragments arranged by size:
1245 104 82
AseI AT'TA_AT
Cuts at: 0 572 1431
Size: 572 859
AvrII C'CTAG_G
Cuts at: 0 1134 1431
Size: 1134 297
BanI G'GyrC_C
Cuts at: 0 822 1431
Size: 822 609
BbsI GAAGACnn'nnnn_
Cuts at: 0 1 1289 1431
Size: 1 1288 142
Fragments arranged by size:
1288 142 1
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1032 1066 1431
Size: 1032 34 365
Fragments arranged by size:
1032 365 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 609 1105 1431
Size: 609 496 326
Fragments arranged by size:
609 496 326
BlpI GC'TnA_GC
Cuts at: 0 45 1431
Size: 45 1386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 432 827 1245 1431
Size: 271 161 395 418 186
Fragments arranged by size:
418 395 271 186 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 1431
Size: 740 691
BsaI GGTCTCn'nnnn_
Cuts at: 0 679 805 1431
Size: 679 126 626
Fragments arranged by size:
679 626 126
BsaHI Gr'CG_yC
Cuts at: 0 856 1431
Size: 856 575
BsmI GAATG_Cn'
Cuts at: 0 773 1431
Size: 773 658
BspHI T'CATG_A
Cuts at: 0 651 1431
Size: 651 780
BstYI r'GATC_y
Cuts at: 0 1354 1431
Size: 1354 77
Bsu36I CC'TnA_GG
Cuts at: 0 955 1431
Size: 955 476
BtgI C'CryG_G
Cuts at: 0 827 1250 1431
Size: 827 423 181
Fragments arranged by size:
827 423 181
ClaI AT'CG_AT
Cuts at: 0 1163 1431
Size: 1163 268
DraI TTT'AAA
Cuts at: 0 927 1065 1431
Size: 927 138 366
Fragments arranged by size:
927 366 138
EaeI y'GGCC_r
Cuts at: 0 660 997 1431
Size: 660 337 434
Fragments arranged by size:
660 434 337
EarI CTCTTCn'nnn_
Cuts at: 0 419 993 1010 1234 1402 1431
Size: 419 574 17 224 168 29
Fragments arranged by size:
574 419 224 168 29 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 736 1431
Size: 736 695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 282 432 716 827 995 1245 1301
Size: 271 11 150 284 111 168 250 56
Cuts at: 1301 1431
Size: 130
Fragments arranged by size:
284 271 250 168 150 130 111 56
11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 563 1431
Size: 563 868
EcoO109I rG'GnC_Cy
Cuts at: 0 1047 1431
Size: 1047 384
FspI TGC'GCA
Cuts at: 0 262 1431
Size: 262 1169
HindIII A'AGCT_T
Cuts at: 0 698 961 1431
Size: 698 263 470
Fragments arranged by size:
698 470 263
KpnI G_GTAC'C
Cuts at: 0 826 1431
Size: 826 605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1171 1346 1431
Size: 266 905 175 85
Fragments arranged by size:
905 266 175 85
MscI TGG'CCA
Cuts at: 0 662 999 1431
Size: 662 337 432
Fragments arranged by size:
662 432 337
MslI CAynn'nnrTG
Cuts at: 0 88 551 590 1431
Size: 88 463 39 841
Fragments arranged by size:
841 463 88 39
MspA1I CmG'CkG
Cuts at: 0 1418 1431
Size: 1418 13
NcoI C'CATG_G
Cuts at: 0 1250 1431
Size: 1250 181
NdeI CA'TA_TG
Cuts at: 0 1029 1431
Size: 1029 402
PasI CC'CwG_GG
Cuts at: 0 559 669 1431
Size: 559 110 762
Fragments arranged by size:
762 559 110
PflMI CCAn_nnn'nTGG
Cuts at: 0 61 669 850 1431
Size: 61 608 181 581
Fragments arranged by size:
608 581 181 61
PpuMI rG'GwC_Cy
Cuts at: 0 1047 1431
Size: 1047 384
PshAI GACnn'nnGTC
Cuts at: 0 170 290 1431
Size: 170 120 1141
Fragments arranged by size:
1141 170 120
PvuII CAG'CTG
Cuts at: 0 1418 1431
Size: 1418 13
SmlI C'TyrA_G
Cuts at: 0 755 1431
Size: 755 676
StyI C'CwwG_G
Cuts at: 0 210 1134 1250 1431
Size: 210 924 116 181
Fragments arranged by size:
924 210 181 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 206 238 1431
Size: 206 32 1193
Fragments arranged by size:
1193 206 32
XbaI T'CTAG_A
Cuts at: 0 111 1431
Size: 111 1320
XmnI GAAnn'nnTTC
Cuts at: 0 1286 1431
Size: 1286 145
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI AseI AvrII BanI BbsI
BcgI BciVI BlpI BpmI BpuEI BsaI BsaHI BsmI
BspHI BstYI Bsu36I BtgI ClaI DraI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FspI HindIII KpnI MmeI
MscI MslI MspA1I NcoI NdeI PasI PflMI PpuMI
PshAI PvuII SmlI StyI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI BaeI BamHI BanII BbeI BbvCI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI CspCI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspAI HaeII Hin4I HincII
HpaI KasI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XcmI XhoI XmaI ZraI