Restriction map for
pFN21AE4456
(Linear) (Six-base) MAPSORT of: KIEE4456.seq Check: 3231 from: 1 to: 1425
KIEE4456.orf
With 182 enzymes: SgfI *
March 22, 2012 18:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 478 1425
Size: 478 947
Acc65I G'GTAC_C
Cuts at: 0 795 1425
Size: 795 630
AfeI AGC'GCT
Cuts at: 0 251 1425
Size: 251 1174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1412 1425
Size: 1412 13
AlwNI CAG_nnn'CTG
Cuts at: 0 588 1425
Size: 588 837
ApaI G_GGCC'C
Cuts at: 0 57 1425
Size: 57 1368
ApoI r'AATT_y
Cuts at: 0 604 921 1132 1167 1425
Size: 604 317 211 35 258
Fragments arranged by size:
604 317 258 211 35
AvaI C'yCGr_G
Cuts at: 0 1149 1425
Size: 1149 276
BamHI G'GATC_C
Cuts at: 0 1038 1425
Size: 1038 387
BanI G'GyrC_C
Cuts at: 0 795 1425
Size: 795 630
BanII G_rGCy'C
Cuts at: 0 57 635 1425
Size: 57 578 790
Fragments arranged by size:
790 578 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 139 1425
Size: 139 1286
BglI GCCn_nnn'nGGC
Cuts at: 0 763 1358 1425
Size: 763 595 67
Fragments arranged by size:
763 595 67
Bme1580I G_kGCm'C
Cuts at: 0 57 1425
Size: 57 1368
BmrI ACTGGGnnnn_n'
Cuts at: 0 1269 1425
Size: 1269 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1403 1425
Size: 880 523 22
Fragments arranged by size:
880 523 22
Bpu10I CC'TnA_GC
Cuts at: 0 629 1425
Size: 629 796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 1425
Size: 513 912
BsaI GGTCTCn'nnnn_
Cuts at: 0 658 1425
Size: 658 767
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 617 647 1425
Size: 617 30 778
Fragments arranged by size:
778 617 30
BseYI C'CCAG_C
Cuts at: 0 92 108 1425
Size: 92 16 1317
Fragments arranged by size:
1317 92 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1425
Size: 1397 28
BsiHKAI G_wGCw'C
Cuts at: 0 635 1425
Size: 635 790
BsmI GAATG_Cn'
Cuts at: 0 886 1425
Size: 886 539
Bsp1286I G_dGCh'C
Cuts at: 0 57 635 1425
Size: 57 578 790
Fragments arranged by size:
790 578 57
BspHI T'CATG_A
Cuts at: 0 1137 1425
Size: 1137 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 478 891 1298 1425
Size: 478 413 407 127
Fragments arranged by size:
478 413 407 127
BsrDI GCAATG_nn'
Cuts at: 0 348 959 1425
Size: 348 611 466
Fragments arranged by size:
611 466 348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 1425
Size: 576 849
BstYI r'GATC_y
Cuts at: 0 1038 1267 1425
Size: 1038 229 158
Fragments arranged by size:
1038 229 158
Bsu36I CC'TnA_GG
Cuts at: 0 284 1425
Size: 284 1141
BtgI C'CryG_G
Cuts at: 0 407 668 986 1178 1425
Size: 407 261 318 192 247
Fragments arranged by size:
407 318 261 247 192
BtsI GCAGTG_nn'
Cuts at: 0 38 736 1425
Size: 38 698 689
Fragments arranged by size:
698 689 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 218 253 326 361 429 464 1425
Size: 218 35 73 35 68 35 961
Fragments arranged by size:
961 218 73 68 35 35 35
DraI TTT'AAA
Cuts at: 0 27 1173 1425
Size: 27 1146 252
Fragments arranged by size:
1146 252 27
EaeI y'GGCC_r
Cuts at: 0 465 692 869 983 1425
Size: 465 227 177 114 442
Fragments arranged by size:
465 442 227 177 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 982 1425
Size: 982 443
EcoICRI GAG'CTC
Cuts at: 0 633 1425
Size: 633 792
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1403 1425
Size: 880 523 22
Fragments arranged by size:
880 523 22
EcoO109I rG'GnC_Cy
Cuts at: 0 54 1425
Size: 54 1371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 799 1425
Size: 799 626
HaeII r_GCGC'y
Cuts at: 0 253 1425
Size: 253 1172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 378 410 490 522 540 572 931 963
Size: 378 32 80 32 18 32 359 32
Cuts at: 963 1425
Size: 462
Fragments arranged by size:
462 378 359 80 32 32 32 32
18
HindIII A'AGCT_T
Cuts at: 0 1142 1412 1425
Size: 1142 270 13
Fragments arranged by size:
1142 270 13
KpnI G_GTAC'C
Cuts at: 0 799 1425
Size: 799 626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1209 1425
Size: 1209 216
MscI TGG'CCA
Cuts at: 0 467 871 985 1425
Size: 467 404 114 440
Fragments arranged by size:
467 440 404 114
MslI CAynn'nnrTG
Cuts at: 0 48 1425
Size: 48 1377
MspA1I CmG'CkG
Cuts at: 0 112 697 916 1381 1425
Size: 112 585 219 465 44
Fragments arranged by size:
585 465 219 112 44
NcoI C'CATG_G
Cuts at: 0 407 668 986 1178 1425
Size: 407 261 318 192 247
Fragments arranged by size:
407 318 261 247 192
NdeI CA'TA_TG
Cuts at: 0 1071 1425
Size: 1071 354
PasI CC'CwG_GG
Cuts at: 0 1278 1425
Size: 1278 147
PshAI GACnn'nnGTC
Cuts at: 0 277 1425
Size: 277 1148
PsiI TTA'TAA
Cuts at: 0 995 1425
Size: 995 430
PspOMI G'GGCC_C
Cuts at: 0 53 1425
Size: 53 1372
PstI C_TGCA'G
Cuts at: 0 684 1309 1425
Size: 684 625 116
Fragments arranged by size:
684 625 116
PvuII CAG'CTG
Cuts at: 0 112 916 1381 1425
Size: 112 804 465 44
Fragments arranged by size:
804 465 112 44
SacI G_AGCT'C
Cuts at: 0 635 1425
Size: 635 790
SfcI C'TryA_G
Cuts at: 0 680 1305 1425
Size: 680 625 120
Fragments arranged by size:
680 625 120
SmlI C'TyrA_G
Cuts at: 0 528 1425
Size: 528 897
StyI C'CwwG_G
Cuts at: 0 363 407 668 986 1178 1223 1425
Size: 363 44 261 318 192 45 202
Fragments arranged by size:
363 318 261 202 192 45 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 701 1301 1425
Size: 701 600 124
Fragments arranged by size:
701 600 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 617 649 1425
Size: 617 32 776
Fragments arranged by size:
776 617 32
XbaI T'CTAG_A
Cuts at: 0 1089 1425
Size: 1089 336
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 666 1425
Size: 666 759
Enzymes that do cut:
AarI Acc65I AfeI AlfI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BglI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaXI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BstXI BstYI Bsu36I BtgI
BtsI CspCI DraI EaeI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HindIII KpnI MmeI MscI MslI
MspA1I NcoI NdeI PasI PshAI PsiI PspOMI PstI
PvuII SacI SfcI SmlI StyI TaqII TstI XbaI
XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BseRI
BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraIII
DrdI EagI EarI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KasI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XhoI
XmaI XmnI ZraI