Restriction map for
pFN21AE4455
(Linear) (Six-base) MAPSORT of: KIEE4455.seq Check: 4669 from: 1 to: 1425
KIEE4455.orf
With 182 enzymes: SgfI *
March 22, 2012 18:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 473 1286 1425
Size: 473 813 139
Fragments arranged by size:
813 473 139
AccI GT'mk_AC
Cuts at: 0 161 1425
Size: 161 1264
Acc65I G'GTAC_C
Cuts at: 0 869 911 1425
Size: 869 42 514
Fragments arranged by size:
869 514 42
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1425
Size: 895 530
AfeI AGC'GCT
Cuts at: 0 1381 1425
Size: 1381 44
AflIII A'CryG_T
Cuts at: 0 332 1425
Size: 332 1093
AleI CACnn'nnGTG
Cuts at: 0 540 1425
Size: 540 885
AlwNI CAG_nnn'CTG
Cuts at: 0 623 966 1425
Size: 623 343 459
Fragments arranged by size:
623 459 343
ApoI r'AATT_y
Cuts at: 0 628 1425
Size: 628 797
AvaI C'yCGr_G
Cuts at: 0 727 1425
Size: 727 698
BamHI G'GATC_C
Cuts at: 0 530 1425
Size: 530 895
BanI G'GyrC_C
Cuts at: 0 722 869 911 1166 1425
Size: 722 147 42 255 259
Fragments arranged by size:
722 259 255 147 42
BanII G_rGCy'C
Cuts at: 0 785 1425
Size: 785 640
BbeI G_GCGC'C
Cuts at: 0 726 1425
Size: 726 699
BbsI GAAGACnn'nnnn_
Cuts at: 0 883 1425
Size: 883 542
BglI GCCn_nnn'nGGC
Cuts at: 0 1037 1425
Size: 1037 388
BmgBI CAC'GTC
Cuts at: 0 335 1425
Size: 335 1090
BmrI ACTGGGnnnn_n'
Cuts at: 0 1383 1425
Size: 1383 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 1425
Size: 59 1366
BsaAI yAC'GTr
Cuts at: 0 375 840 1425
Size: 375 465 585
Fragments arranged by size:
585 465 375
BsaBI GATnn'nnATC
Cuts at: 0 664 1425
Size: 664 761
BsaHI Gr'CG_yC
Cuts at: 0 50 723 1016 1425
Size: 50 673 293 409
Fragments arranged by size:
673 409 293 50
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 1425
Size: 445 980
BseYI C'CCAG_C
Cuts at: 0 290 920 1026 1425
Size: 290 630 106 399
Fragments arranged by size:
630 399 290 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1425
Size: 1009 416
BsiEI CG_ry'CG
Cuts at: 0 1368 1425
Size: 1368 57
BsiHKAI G_wGCw'C
Cuts at: 0 785 1141 1346 1425
Size: 785 356 205 79
Fragments arranged by size:
785 356 205 79
BsmBI CGTCTCn'nnnn_
Cuts at: 0 341 576 1425
Size: 341 235 849
Fragments arranged by size:
849 341 235
Bsp1286I G_dGCh'C
Cuts at: 0 785 1141 1346 1425
Size: 785 356 205 79
Fragments arranged by size:
785 356 205 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 1286 1425
Size: 473 813 139
Fragments arranged by size:
813 473 139
BsrFI r'CCGG_y
Cuts at: 0 205 1425
Size: 205 1220
BsrGI T'GTAC_A
Cuts at: 0 329 1425
Size: 329 1096
BstEII G'GTnAC_C
Cuts at: 0 309 1425
Size: 309 1116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 542 807 813 1425
Size: 542 265 6 612
Fragments arranged by size:
612 542 265 6
BstYI r'GATC_y
Cuts at: 0 530 1425
Size: 530 895
BtgI C'CryG_G
Cuts at: 0 584 806 1010 1425
Size: 584 222 204 415
Fragments arranged by size:
584 415 222 204
BtsI GCAGTG_nn'
Cuts at: 0 88 535 1400 1425
Size: 88 447 865 25
Fragments arranged by size:
865 447 88 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 64 99 1425
Size: 64 35 1326
Fragments arranged by size:
1326 64 35
DraIII CAC_nnn'GTG
Cuts at: 0 987 1011 1425
Size: 987 24 414
Fragments arranged by size:
987 414 24
EaeI y'GGCC_r
Cuts at: 0 1058 1272 1307 1365 1425
Size: 1058 214 35 58 60
Fragments arranged by size:
1058 214 60 58 35
EagI C'GGCC_G
Cuts at: 0 1365 1425
Size: 1365 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1072 1140 1425
Size: 1072 68 285
Fragments arranged by size:
1072 285 68
EcoICRI GAG'CTC
Cuts at: 0 783 1425
Size: 783 642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1425
Size: 895 530
EcoO109I rG'GnC_Cy
Cuts at: 0 1074 1425
Size: 1074 351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 615 729 1425
Size: 615 114 696
Fragments arranged by size:
696 615 114
HaeII r_GCGC'y
Cuts at: 0 617 726 1101 1383 1425
Size: 617 109 375 282 42
Fragments arranged by size:
617 375 282 109 42
HincII GTy'rAC
Cuts at: 0 819 1425
Size: 819 606
KasI G'GCGC_C
Cuts at: 0 722 1425
Size: 722 703
KpnI G_GTAC'C
Cuts at: 0 873 915 1425
Size: 873 42 510
Fragments arranged by size:
873 510 42
MscI TGG'CCA
Cuts at: 0 1060 1309 1425
Size: 1060 249 116
Fragments arranged by size:
1060 249 116
MslI CAynn'nnrTG
Cuts at: 0 540 791 838 1303 1425
Size: 540 251 47 465 122
Fragments arranged by size:
540 465 251 122 47
MspA1I CmG'CkG
Cuts at: 0 194 777 993 1328 1349 1425
Size: 194 583 216 335 21 76
Fragments arranged by size:
583 335 216 194 76 21
NarI GG'CG_CC
Cuts at: 0 723 1425
Size: 723 702
NcoI C'CATG_G
Cuts at: 0 584 806 1425
Size: 584 222 619
Fragments arranged by size:
619 584 222
NspI r_CATG'y
Cuts at: 0 238 1425
Size: 238 1187
PfoI T'CCnGG_A
Cuts at: 0 386 1334 1425
Size: 386 948 91
Fragments arranged by size:
948 386 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 57 89 1425
Size: 57 32 1336
Fragments arranged by size:
1336 57 32
PpuMI rG'GwC_Cy
Cuts at: 0 1074 1425
Size: 1074 351
PstI C_TGCA'G
Cuts at: 0 411 830 962 1425
Size: 411 419 132 463
Fragments arranged by size:
463 419 411 132
PvuII CAG'CTG
Cuts at: 0 194 993 1425
Size: 194 799 432
Fragments arranged by size:
799 432 194
SacI G_AGCT'C
Cuts at: 0 785 1425
Size: 785 640
SbfI CC_TGCA'GG
Cuts at: 0 411 830 1425
Size: 411 419 595
Fragments arranged by size:
595 419 411
SexAI A'CCwGG_T
Cuts at: 0 865 1425
Size: 865 560
SfcI C'TryA_G
Cuts at: 0 407 826 958 1425
Size: 407 419 132 467
Fragments arranged by size:
467 419 407 132
SfoI GGC'GCC
Cuts at: 0 724 1425
Size: 724 701
SmaI CCC'GGG
Cuts at: 0 729 1425
Size: 729 696
SmlI C'TyrA_G
Cuts at: 0 74 1425
Size: 74 1351
SnaBI TAC'GTA
Cuts at: 0 375 1425
Size: 375 1050
StyI C'CwwG_G
Cuts at: 0 558 584 806 1124 1425
Size: 558 26 222 318 301
Fragments arranged by size:
558 318 301 222 26
TatI w'GTAC_w
Cuts at: 0 188 329 968 1425
Size: 188 141 639 457
Fragments arranged by size:
639 457 188 141
Tth111I GACn'n_nGTC
Cuts at: 0 223 931 1425
Size: 223 708 494
Fragments arranged by size:
708 494 223
XbaI T'CTAG_A
Cuts at: 0 42 1425
Size: 42 1383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 582 1425
Size: 582 843
XmaI C'CCGG_G
Cuts at: 0 727 1425
Size: 727 698
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlwNI
ApoI AvaI BamHI BanI BanII BbeI BbsI BglI
BmgBI BmrI BpuEI BsaAI BsaBI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI
BstEII BstXI BstYI BtgI BtsI CspCI DraIII EaeI
EagI EciI EcoICRI Eco57MI EcoO109I FalI HaeII HincII
KasI KpnI MscI MslI MspA1I NarI NcoI NspI
PfoI PpiI PpuMI PstI PvuII SacI SbfI SexAI
SfcI SfoI SmaI SmlI SnaBI StyI TatI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmtI BplI
BpmI Bpu10I BsaI BsaWI BsaXI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI DraI DrdI EarI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HindIII HpaI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI ScaI SfiI SgfI SgrAI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI XhoI XmnI
ZraI