Restriction map for
pFN21AE4451
(Linear) (Six-base) MAPSORT of: KIEE4451.seq Check: 2189 from: 1 to: 1404
KIEE4451.orf
With 182 enzymes: SgfI *
March 22, 2012 18:04 ..
AccI GT'mk_AC
Cuts at: 0 620 1109 1404
Size: 620 489 295
Fragments arranged by size:
620 489 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 526 842 1404
Size: 356 170 316 562
Fragments arranged by size:
562 356 316 170
AflII C'TTAA_G
Cuts at: 0 151 1404
Size: 151 1253
AflIII A'CryG_T
Cuts at: 0 363 1404
Size: 363 1041
AlwNI CAG_nnn'CTG
Cuts at: 0 379 1404
Size: 379 1025
ApaI G_GGCC'C
Cuts at: 0 23 1404
Size: 23 1381
ApoI r'AATT_y
Cuts at: 0 223 559 1261 1404
Size: 223 336 702 143
Fragments arranged by size:
702 336 223 143
AseI AT'TA_AT
Cuts at: 0 1372 1404
Size: 1372 32
BanI G'GyrC_C
Cuts at: 0 1044 1404
Size: 1044 360
BanII G_rGCy'C
Cuts at: 0 23 791 1404
Size: 23 768 613
Fragments arranged by size:
768 613 23
BbeI G_GCGC'C
Cuts at: 0 1048 1404
Size: 1048 356
BbsI GAAGACnn'nnnn_
Cuts at: 0 804 873 987 1404
Size: 804 69 114 417
Fragments arranged by size:
804 417 114 69
BciVI GTATCCnnnnn_n'
Cuts at: 0 719 1404
Size: 719 685
Bme1580I G_kGCm'C
Cuts at: 0 23 1404
Size: 23 1381
BsaAI yAC'GTr
Cuts at: 0 364 1404
Size: 364 1040
BsaHI Gr'CG_yC
Cuts at: 0 1045 1404
Size: 1045 359
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 465 495 1404
Size: 465 30 909
Fragments arranged by size:
909 465 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 837 1404
Size: 837 567
Bsp1286I G_dGCh'C
Cuts at: 0 23 791 1404
Size: 23 768 613
Fragments arranged by size:
768 613 23
BspHI T'CATG_A
Cuts at: 0 1220 1404
Size: 1220 184
BsrFI r'CCGG_y
Cuts at: 0 486 1404
Size: 486 918
BssHII G'CGCG_C
Cuts at: 0 26 95 1404
Size: 26 69 1309
Fragments arranged by size:
1309 69 26
BstEII G'GTnAC_C
Cuts at: 0 987 1404
Size: 987 417
BstYI r'GATC_y
Cuts at: 0 1324 1404
Size: 1324 80
BtgI C'CryG_G
Cuts at: 0 1040 1404
Size: 1040 364
EarI CTCTTCn'nnn_
Cuts at: 0 893 1404
Size: 893 511
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1404
Size: 22 1382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 526 842 1404
Size: 356 170 316 562
Fragments arranged by size:
562 356 316 170
EcoO109I rG'GnC_Cy
Cuts at: 0 19 20 1404
Size: 19 1 1384
Fragments arranged by size:
1384 19 1
HaeII r_GCGC'y
Cuts at: 0 69 744 1048 1404
Size: 69 675 304 356
Fragments arranged by size:
675 356 304 69
KasI G'GCGC_C
Cuts at: 0 1044 1404
Size: 1044 360
MfeI C'AATT_G
Cuts at: 0 244 1316 1404
Size: 244 1072 88
Fragments arranged by size:
1072 244 88
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1302 1404
Size: 1302 102
MslI CAynn'nnrTG
Cuts at: 0 203 1404
Size: 203 1201
MspA1I CmG'CkG
Cuts at: 0 54 698 1404
Size: 54 644 706
Fragments arranged by size:
706 644 54
NarI GG'CG_CC
Cuts at: 0 1045 1404
Size: 1045 359
NcoI C'CATG_G
Cuts at: 0 1040 1404
Size: 1040 364
PsiI TTA'TAA
Cuts at: 0 772 1355 1404
Size: 772 583 49
Fragments arranged by size:
772 583 49
PspOMI G'GGCC_C
Cuts at: 0 19 1404
Size: 19 1385
PvuII CAG'CTG
Cuts at: 0 54 698 1404
Size: 54 644 706
Fragments arranged by size:
706 644 54
ScaI AGT'ACT
Cuts at: 0 863 1404
Size: 863 541
SfoI GGC'GCC
Cuts at: 0 1046 1404
Size: 1046 358
SmlI C'TyrA_G
Cuts at: 0 151 1404
Size: 151 1253
StyI C'CwwG_G
Cuts at: 0 1040 1404
Size: 1040 364
TatI w'GTAC_w
Cuts at: 0 804 861 1404
Size: 804 57 543
Fragments arranged by size:
804 543 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 465 497 1404
Size: 465 32 907
Fragments arranged by size:
907 465 32
Tth111I GACn'n_nGTC
Cuts at: 0 379 975 1404
Size: 379 596 429
Fragments arranged by size:
596 429 379
XbaI T'CTAG_A
Cuts at: 0 603 1165 1404
Size: 603 562 239
Fragments arranged by size:
603 562 239
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1047 1404
Size: 1047 357
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApaI ApoI AseI
BanI BanII BbeI BbsI BciVI Bme1580I BsaAI BsaHI
BsaXI BsgI Bsp1286I BspHI BsrFI BssHII BstEII BstYI
BtgI EarI EciI Eco57MI EcoO109I HaeII KasI MfeI
MmeI MslI MspA1I NarI NcoI PsiI PspOMI PvuII
ScaI SfoI SmlI StyI TatI TstI Tth111I XbaI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MluI MscI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII XhoI
XmaI XmnI ZraI