Restriction map for
pFN21AE4448
(Linear) (Six-base) MAPSORT of: KIEE4448.seq Check: 3530 from: 1 to: 1389
KIEE4448.orf
With 182 enzymes: SgfI *
March 22, 2012 18:03 ..
AccI GT'mk_AC
Cuts at: 0 1153 1389
Size: 1153 236
Acc65I G'GTAC_C
Cuts at: 0 986 1354 1389
Size: 986 368 35
Fragments arranged by size:
986 368 35
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1389
Size: 373 1016
AflIII A'CryG_T
Cuts at: 0 532 1325 1389
Size: 532 793 64
Fragments arranged by size:
793 532 64
AhdI GACnn_n'nnGTC
Cuts at: 0 423 1311 1389
Size: 423 888 78
Fragments arranged by size:
888 423 78
AleI CACnn'nnGTG
Cuts at: 0 226 1389
Size: 226 1163
AlwNI CAG_nnn'CTG
Cuts at: 0 51 1020 1389
Size: 51 969 369
Fragments arranged by size:
969 369 51
ApaI G_GGCC'C
Cuts at: 0 257 1389
Size: 257 1132
ApaLI G'TGCA_C
Cuts at: 0 694 1389
Size: 694 695
AvaI C'yCGr_G
Cuts at: 0 494 965 1389
Size: 494 471 424
Fragments arranged by size:
494 471 424
BamHI G'GATC_C
Cuts at: 0 506 1389
Size: 506 883
BanI G'GyrC_C
Cuts at: 0 986 1354 1389
Size: 986 368 35
Fragments arranged by size:
986 368 35
BanII G_rGCy'C
Cuts at: 0 257 344 660 886 1389
Size: 257 87 316 226 503
Fragments arranged by size:
503 316 257 226 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 916 1120 1389
Size: 916 204 269
Fragments arranged by size:
916 269 204
BbvCI CC'TCA_GC
Cuts at: 0 10 1389
Size: 10 1379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 956 990 1389
Size: 956 34 399
Fragments arranged by size:
956 399 34
BfrBI ATG'CAT
Cuts at: 0 666 1389
Size: 666 723
BglI GCCn_nnn'nGGC
Cuts at: 0 1201 1294 1389
Size: 1201 93 95
Fragments arranged by size:
1201 95 93
Bme1580I G_kGCm'C
Cuts at: 0 132 257 501 698 1389
Size: 132 125 244 197 691
Fragments arranged by size:
691 244 197 132 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 674 1160 1389
Size: 86 588 486 229
Fragments arranged by size:
588 486 229 86
Bpu10I CC'TnA_GC
Cuts at: 0 10 885 1097 1389
Size: 10 875 212 292
Fragments arranged by size:
875 292 212 10
BsaHI Gr'CG_yC
Cuts at: 0 35 1389
Size: 35 1354
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 613 643 1389
Size: 613 30 746
Fragments arranged by size:
746 613 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 479 482 704 790 1018 1389
Size: 479 3 222 86 228 371
Fragments arranged by size:
479 371 228 222 86 3
BseYI C'CCAG_C
Cuts at: 0 88 264 914 1389
Size: 88 176 650 475
Fragments arranged by size:
650 475 176 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1389
Size: 331 1058
BsiHKAI G_wGCw'C
Cuts at: 0 698 1389
Size: 698 691
BsmI GAATG_Cn'
Cuts at: 0 668 1389
Size: 668 721
Bsp1286I G_dGCh'C
Cuts at: 0 132 257 344 501 660 698 886 1389
Size: 132 125 87 157 159 38 188 503
Fragments arranged by size:
503 188 159 157 132 125 87 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 911 1389
Size: 911 478
BsrFI r'CCGG_y
Cuts at: 0 410 1389
Size: 410 979
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1297 1389
Size: 1297 92
BstYI r'GATC_y
Cuts at: 0 506 797 1389
Size: 506 291 592
Fragments arranged by size:
592 506 291
BstZ17I GTA'TAC
Cuts at: 0 1154 1389
Size: 1154 235
BtgI C'CryG_G
Cuts at: 0 1121 1389
Size: 1121 268
BtsI GCAGTG_nn'
Cuts at: 0 112 1389
Size: 112 1277
ClaI AT'CG_AT
Cuts at: 0 1260 1389
Size: 1260 129
EaeI y'GGCC_r
Cuts at: 0 682 1389
Size: 682 707
EarI CTCTTCn'nnn_
Cuts at: 0 761 1389
Size: 761 628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 373 674 1160 1389
Size: 86 287 301 486 229
Fragments arranged by size:
486 301 287 229 86
EcoO109I rG'GnC_Cy
Cuts at: 0 254 393 702 900 1389
Size: 254 139 309 198 489
Fragments arranged by size:
489 309 254 198 139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 865 1389
Size: 865 524
HaeII r_GCGC'y
Cuts at: 0 10 1389
Size: 10 1379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 634 666 1253 1260 1285 1292
Size: 409 32 193 32 587 7 25 7
Cuts at: 1292 1297 1329 1389
Size: 5 32 60
Fragments arranged by size:
587 409 193 60 32 32 32 25
7 7 5
KpnI G_GTAC'C
Cuts at: 0 990 1358 1389
Size: 990 368 31
Fragments arranged by size:
990 368 31
MfeI C'AATT_G
Cuts at: 0 224 815 1389
Size: 224 591 574
Fragments arranged by size:
591 574 224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 1389
Size: 752 637
MslI CAynn'nnrTG
Cuts at: 0 226 556 1389
Size: 226 330 833
Fragments arranged by size:
833 330 226
NcoI C'CATG_G
Cuts at: 0 1121 1389
Size: 1121 268
NsiI A_TGCA'T
Cuts at: 0 668 1389
Size: 668 721
NspI r_CATG'y
Cuts at: 0 571 670 1329 1389
Size: 571 99 659 60
Fragments arranged by size:
659 571 99 60
PciI A'CATG_T
Cuts at: 0 1325 1389
Size: 1325 64
PflMI CCAn_nnn'nTGG
Cuts at: 0 552 1389
Size: 552 837
PfoI T'CCnGG_A
Cuts at: 0 1176 1389
Size: 1176 213
PpuMI rG'GwC_Cy
Cuts at: 0 702 900 1389
Size: 702 198 489
Fragments arranged by size:
702 489 198
PspOMI G'GGCC_C
Cuts at: 0 253 1389
Size: 253 1136
SexAI A'CCwGG_T
Cuts at: 0 935 1229 1389
Size: 935 294 160
Fragments arranged by size:
935 294 160
SmlI C'TyrA_G
Cuts at: 0 965 1389
Size: 965 424
SpeI A'CTAG_T
Cuts at: 0 288 1389
Size: 288 1101
SphI G_CATG'C
Cuts at: 0 670 1389
Size: 670 719
StuI AGG'CCT
Cuts at: 0 448 1389
Size: 448 941
StyI C'CwwG_G
Cuts at: 0 193 579 711 1121 1208 1389
Size: 193 386 132 410 87 181
Fragments arranged by size:
410 386 193 181 132 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 1389
Size: 278 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 1389
Size: 491 32 866
Fragments arranged by size:
866 491 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 122 570 1389
Size: 122 448 819
Fragments arranged by size:
819 448 122
XhoI C'TCGA_G
Cuts at: 0 965 1389
Size: 965 424
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AleI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BfrBI BglI Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstXI
BstYI BstZ17I BtgI BtsI ClaI EaeI EarI Eco57MI
EcoO109I FalI HaeII Hin4I KpnI MfeI MmeI MslI
NcoI NsiI NspI PciI PflMI PfoI PpuMI PspOMI
SexAI SmlI SpeI SphI StuI StyI TaqII TstI
XcmI XhoI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI
BclI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII Bsu36I BtgZI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI SspI SwaI TatI Tth111I XbaI XmaI
XmnI ZraI