Restriction map for
pFN21AE4444
(Linear) (Six-base) MAPSORT of: KIEE4444.seq Check: 8563 from: 1 to: 1380
KIEE4444.orf
With 182 enzymes: SgfI *
March 22, 2012 18:03 ..
AccI GT'mk_AC
Cuts at: 0 1262 1380
Size: 1262 118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 1380
Size: 734 646
ApaI G_GGCC'C
Cuts at: 0 485 609 1380
Size: 485 124 771
Fragments arranged by size:
771 485 124
ApaLI G'TGCA_C
Cuts at: 0 1058 1380
Size: 1058 322
ApoI r'AATT_y
Cuts at: 0 640 1380
Size: 640 740
BamHI G'GATC_C
Cuts at: 0 1047 1380
Size: 1047 333
BanI G'GyrC_C
Cuts at: 0 571 1380
Size: 571 809
BanII G_rGCy'C
Cuts at: 0 485 609 1250 1380
Size: 485 124 641 130
Fragments arranged by size:
641 485 130 124
BbsI GAAGACnn'nnnn_
Cuts at: 0 630 1380
Size: 630 750
BbvCI CC'TCA_GC
Cuts at: 0 226 1380
Size: 226 1154
BciVI GTATCCnnnnn_n'
Cuts at: 0 1185 1380
Size: 1185 195
BlpI GC'TnA_GC
Cuts at: 0 181 1380
Size: 181 1199
Bme1580I G_kGCm'C
Cuts at: 0 485 576 609 1062 1325 1380
Size: 485 91 33 453 263 55
Fragments arranged by size:
485 453 263 91 55 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 612 1380
Size: 166 446 768
Fragments arranged by size:
768 446 166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1380
Size: 634 746
Bpu10I CC'TnA_GC
Cuts at: 0 226 1380
Size: 226 1154
BsaI GGTCTCn'nnnn_
Cuts at: 0 300 1380
Size: 300 1080
BsaAI yAC'GTr
Cuts at: 0 834 1380
Size: 834 546
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 1088 1118 1380
Size: 145 30 913 30 262
Fragments arranged by size:
913 262 145 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 932 1109 1380
Size: 932 177 271
Fragments arranged by size:
932 271 177
BseYI C'CCAG_C
Cuts at: 0 196 618 1380
Size: 196 422 762
Fragments arranged by size:
762 422 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 1302 1380
Size: 723 579 78
Fragments arranged by size:
723 579 78
BsiHKAI G_wGCw'C
Cuts at: 0 677 927 1062 1380
Size: 677 250 135 318
Fragments arranged by size:
677 318 250 135
BsmI GAATG_Cn'
Cuts at: 0 743 1380
Size: 743 637
Bsp1286I G_dGCh'C
Cuts at: 0 485 576 609 677 927 1062 1250 1325
Size: 485 91 33 68 250 135 188 75
Cuts at: 1325 1380
Size: 55
Fragments arranged by size:
485 250 188 135 91 75 68 55
33
BspHI T'CATG_A
Cuts at: 0 332 1380
Size: 332 1048
BsrDI GCAATG_nn'
Cuts at: 0 857 905 1380
Size: 857 48 475
Fragments arranged by size:
857 475 48
BsrFI r'CCGG_y
Cuts at: 0 284 1380
Size: 284 1096
BsrGI T'GTAC_A
Cuts at: 0 206 1313 1380
Size: 206 1107 67
Fragments arranged by size:
1107 206 67
BssSI C'ACGA_G
Cuts at: 0 340 1380
Size: 340 1040
BstEII G'GTnAC_C
Cuts at: 0 580 1380
Size: 580 800
BstXI CCAn_nnnn'nTGG
Cuts at: 0 976 1380
Size: 976 404
BstYI r'GATC_y
Cuts at: 0 1047 1380
Size: 1047 333
Bsu36I CC'TnA_GG
Cuts at: 0 764 1380
Size: 764 616
BtgI C'CryG_G
Cuts at: 0 257 1041 1380
Size: 257 784 339
Fragments arranged by size:
784 339 257
BtsI GCAGTG_nn'
Cuts at: 0 279 913 1380
Size: 279 634 467
Fragments arranged by size:
634 467 279
DraIII CAC_nnn'GTG
Cuts at: 0 683 1380
Size: 683 697
DrdI GACnn_nn'nnGTC
Cuts at: 0 909 1380
Size: 909 471
EaeI y'GGCC_r
Cuts at: 0 24 119 269 1380
Size: 24 95 150 1111
Fragments arranged by size:
1111 150 95 24
EarI CTCTTCn'nnn_
Cuts at: 0 126 559 1255 1380
Size: 126 433 696 125
Fragments arranged by size:
696 433 126 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 734 1380
Size: 634 100 646
Fragments arranged by size:
646 634 100
EcoO109I rG'GnC_Cy
Cuts at: 0 534 768 1380
Size: 534 234 612
Fragments arranged by size:
612 534 234
EcoRI G'AATT_C
Cuts at: 0 640 1380
Size: 640 740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 739 1380
Size: 739 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 496 528 1380
Size: 299 32 165 32 852
Fragments arranged by size:
852 299 165 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 1022 1086 1380
Size: 243 779 64 294
Fragments arranged by size:
779 294 243 64
MscI TGG'CCA
Cuts at: 0 121 1380
Size: 121 1259
MslI CAynn'nnrTG
Cuts at: 0 121 545 1380
Size: 121 424 835
Fragments arranged by size:
835 424 121
MspA1I CmG'CkG
Cuts at: 0 1043 1380
Size: 1043 337
NspI r_CATG'y
Cuts at: 0 939 1005 1380
Size: 939 66 375
Fragments arranged by size:
939 375 66
PasI CC'CwG_GG
Cuts at: 0 324 576 1380
Size: 324 252 804
Fragments arranged by size:
804 324 252
PpuMI rG'GwC_Cy
Cuts at: 0 768 1380
Size: 768 612
PshAI GACnn'nnGTC
Cuts at: 0 172 1380
Size: 172 1208
PspOMI G'GGCC_C
Cuts at: 0 481 605 1380
Size: 481 124 775
Fragments arranged by size:
775 481 124
PstI C_TGCA'G
Cuts at: 0 254 1380
Size: 254 1126
SacII CC_GC'GG
Cuts at: 0 1044 1380
Size: 1044 336
SapI GCTCTTCn'nnn_
Cuts at: 0 1255 1380
Size: 1255 125
SfcI C'TryA_G
Cuts at: 0 250 596 1053 1380
Size: 250 346 457 327
Fragments arranged by size:
457 346 327 250
SnaBI TAC'GTA
Cuts at: 0 834 1380
Size: 834 546
StyI C'CwwG_G
Cuts at: 0 400 1380
Size: 400 980
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 580 1380
Size: 580 800
TatI w'GTAC_w
Cuts at: 0 206 1313 1380
Size: 206 1107 67
Fragments arranged by size:
1107 206 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 1380
Size: 271 32 1077
Fragments arranged by size:
1077 271 32
Enzymes that do cut:
AccI AcuI ApaI ApaLI ApoI BamHI BanI BanII
BbsI BbvCI BciVI BlpI Bme1580I BmrI BpmI Bpu10I
BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI
Eco57MI EcoO109I EcoRI FalI Hin4I MmeI MscI MslI
MspA1I NspI PasI PpuMI PshAI PspOMI PstI SacII
SapI SfcI SnaBI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BcgI BclI BfrBI BglI
BglII BmgBI BmtI BplI BpuEI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI