Restriction map for
pFN21AE4442
(Linear) (Six-base) MAPSORT of: KIEE4442.seq Check: 5934 from: 1 to: 1377
KIEE4442.orf
With 182 enzymes: SgfI *
March 22, 2012 18:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 343 945 1377
Size: 308 35 602 432
Fragments arranged by size:
602 432 308 35
AlwNI CAG_nnn'CTG
Cuts at: 0 23 876 1377
Size: 23 853 501
Fragments arranged by size:
853 501 23
ApaI G_GGCC'C
Cuts at: 0 847 1377
Size: 847 530
ApoI r'AATT_y
Cuts at: 0 1218 1377
Size: 1218 159
AvaI C'yCGr_G
Cuts at: 0 449 646 804 1193 1377
Size: 449 197 158 389 184
Fragments arranged by size:
449 389 197 184 158
BamHI G'GATC_C
Cuts at: 0 225 1377
Size: 225 1152
BanI G'GyrC_C
Cuts at: 0 800 1377
Size: 800 577
BanII G_rGCy'C
Cuts at: 0 319 666 709 847 1377
Size: 319 347 43 138 530
Fragments arranged by size:
530 347 319 138 43
BbeI G_GCGC'C
Cuts at: 0 804 1377
Size: 804 573
BbvCI CC'TCA_GC
Cuts at: 0 763 899 1377
Size: 763 136 478
Fragments arranged by size:
763 478 136
BglI GCCn_nnn'nGGC
Cuts at: 0 624 809 823 1377
Size: 624 185 14 554
Fragments arranged by size:
624 554 185 14
BlpI GC'TnA_GC
Cuts at: 0 1058 1202 1377
Size: 1058 144 175
Fragments arranged by size:
1058 175 144
Bme1580I G_kGCm'C
Cuts at: 0 16 847 1011 1377
Size: 16 831 164 366
Fragments arranged by size:
831 366 164 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 187 191 693 1377
Size: 187 4 502 684
Fragments arranged by size:
684 502 187 4
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 924 1377
Size: 924 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 272 290 377 856 1196 1377
Size: 38 234 18 87 479 340 181
Fragments arranged by size:
479 340 234 181 87 38 18
Bpu10I CC'TnA_GC
Cuts at: 0 763 899 1377
Size: 763 136 478
Fragments arranged by size:
763 478 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1377
Size: 350 1027
BsaI GGTCTCn'nnnn_
Cuts at: 0 992 1377
Size: 992 385
BsaAI yAC'GTr
Cuts at: 0 753 1377
Size: 753 624
BsaHI Gr'CG_yC
Cuts at: 0 801 1377
Size: 801 576
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 603 633 1377
Size: 603 30 744
Fragments arranged by size:
744 603 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 164 199 235 253 271 289 853 1377
Size: 164 35 36 18 18 18 564 524
Fragments arranged by size:
564 524 164 36 35 18 18 18
BseYI C'CCAG_C
Cuts at: 0 16 459 503 1350 1377
Size: 16 443 44 847 27
Fragments arranged by size:
847 443 44 27 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 926 1318 1377
Size: 664 262 392 59
Fragments arranged by size:
664 392 262 59
BsiHKAI G_wGCw'C
Cuts at: 0 716 1377
Size: 716 661
Bsp1286I G_dGCh'C
Cuts at: 0 16 319 666 709 716 847 1011 1377
Size: 16 303 347 43 7 131 164 366
Fragments arranged by size:
366 347 303 164 131 43 16 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 685 1377
Size: 685 692
BsrBI CCG'CTC
Cuts at: 0 567 1377
Size: 567 810
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 1377
Size: 75 1302
BstYI r'GATC_y
Cuts at: 0 189 225 243 261 279 333 360 746
Size: 189 36 18 18 18 54 27 386
Cuts at: 746 1377
Size: 631
Fragments arranged by size:
631 386 189 54 36 27 18 18
18
BtsI GCAGTG_nn'
Cuts at: 0 82 1377
Size: 82 1295
DraIII CAC_nnn'GTG
Cuts at: 0 603 720 1088 1377
Size: 603 117 368 289
Fragments arranged by size:
603 368 289 117
EaeI y'GGCC_r
Cuts at: 0 594 815 824 1377
Size: 594 221 9 553
Fragments arranged by size:
594 553 221 9
EarI CTCTTCn'nnn_
Cuts at: 0 162 197 305 1377
Size: 162 35 108 1072
Fragments arranged by size:
1072 162 108 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 487 1377
Size: 487 890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 272 290 308 343 377 856 945
Size: 38 234 18 18 35 34 479 89
Cuts at: 945 1196 1377
Size: 251 181
Fragments arranged by size:
479 251 234 181 89 38 35 34
18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 53 1377
Size: 53 1324
EcoO109I rG'GnC_Cy
Cuts at: 0 643 843 921 1093 1377
Size: 643 200 78 172 284
Fragments arranged by size:
643 284 200 172 78
EcoRI G'AATT_C
Cuts at: 0 1218 1377
Size: 1218 159
EcoRV GAT'ATC
Cuts at: 0 495 1377
Size: 495 882
FspI TGC'GCA
Cuts at: 0 586 1377
Size: 586 791
HaeII r_GCGC'y
Cuts at: 0 426 804 1377
Size: 426 378 573
Fragments arranged by size:
573 426 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 603 635 1377
Size: 603 32 742
Fragments arranged by size:
742 603 32
HincII GTy'rAC
Cuts at: 0 786 1377
Size: 786 591
KasI G'GCGC_C
Cuts at: 0 800 1377
Size: 800 577
MscI TGG'CCA
Cuts at: 0 596 1377
Size: 596 781
MspA1I CmG'CkG
Cuts at: 0 672 1116 1215 1377
Size: 672 444 99 162
Fragments arranged by size:
672 444 162 99
NarI GG'CG_CC
Cuts at: 0 801 1377
Size: 801 576
PasI CC'CwG_GG
Cuts at: 0 1011 1377
Size: 1011 366
PflMI CCAn_nnn'nTGG
Cuts at: 0 23 1377
Size: 23 1354
PfoI T'CCnGG_A
Cuts at: 0 354 1377
Size: 354 1023
PmlI CAC'GTG
Cuts at: 0 753 1377
Size: 753 624
PpuMI rG'GwC_Cy
Cuts at: 0 643 921 1093 1377
Size: 643 278 172 284
Fragments arranged by size:
643 284 278 172
PshAI GACnn'nnGTC
Cuts at: 0 413 919 1187 1377
Size: 413 506 268 190
Fragments arranged by size:
506 413 268 190
PspOMI G'GGCC_C
Cuts at: 0 843 1377
Size: 843 534
PstI C_TGCA'G
Cuts at: 0 671 696 1377
Size: 671 25 681
Fragments arranged by size:
681 671 25
PvuII CAG'CTG
Cuts at: 0 672 1116 1215 1377
Size: 672 444 99 162
Fragments arranged by size:
672 444 162 99
SanDI GG'GwC_CC
Cuts at: 0 643 1377
Size: 643 734
SapI GCTCTTCn'nnn_
Cuts at: 0 162 1377
Size: 162 1215
SexAI A'CCwGG_T
Cuts at: 0 1095 1377
Size: 1095 282
SfcI C'TryA_G
Cuts at: 0 667 692 1111 1377
Size: 667 25 419 266
Fragments arranged by size:
667 419 266 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 823 1377
Size: 823 554
SfoI GGC'GCC
Cuts at: 0 802 1377
Size: 802 575
SmaI CCC'GGG
Cuts at: 0 451 648 806 1377
Size: 451 197 158 571
Fragments arranged by size:
571 451 197 158
SmlI C'TyrA_G
Cuts at: 0 365 1377
Size: 365 1012
StuI AGG'CCT
Cuts at: 0 59 793 812 1162 1377
Size: 59 734 19 350 215
Fragments arranged by size:
734 350 215 59 19
StyI C'CwwG_G
Cuts at: 0 143 794 1377
Size: 143 651 583
Fragments arranged by size:
651 583 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 228 1377
Size: 228 1149
XmaI C'CCGG_G
Cuts at: 0 449 646 804 1377
Size: 449 197 158 573
Fragments arranged by size:
573 449 197 158
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbvCI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BstAPI BstYI BtsI
DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII KasI MscI MspA1I
NarI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PstI PvuII SanDI SapI SexAI SfcI SfiI SfoI
SmaI SmlI StuI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DrdI EagI EcoICRI FalI FseI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MslI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI