Restriction map for
pFN21AE4439
(Linear) (Six-base) MAPSORT of: KIEE4439.seq Check: 4944 from: 1 to: 1368
KIEE4439.orf
With 182 enzymes: SgfI *
March 22, 2012 18:01 ..
AclI AA'CG_TT
Cuts at: 0 975 1368
Size: 975 393
AflIII A'CryG_T
Cuts at: 0 131 1368
Size: 131 1237
AhdI GACnn_n'nnGTC
Cuts at: 0 8 1368
Size: 8 1360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 419 1368
Size: 419 949
AlwNI CAG_nnn'CTG
Cuts at: 0 968 1076 1238 1368
Size: 968 108 162 130
Fragments arranged by size:
968 162 130 108
ApaI G_GGCC'C
Cuts at: 0 1256 1368
Size: 1256 112
ApoI r'AATT_y
Cuts at: 0 87 688 1368
Size: 87 601 680
Fragments arranged by size:
680 601 87
AseI AT'TA_AT
Cuts at: 0 449 1368
Size: 449 919
AvaI C'yCGr_G
Cuts at: 0 727 1368
Size: 727 641
BamHI G'GATC_C
Cuts at: 0 314 332 1368
Size: 314 18 1036
Fragments arranged by size:
1036 314 18
BanI G'GyrC_C
Cuts at: 0 460 1368
Size: 460 908
BanII G_rGCy'C
Cuts at: 0 1256 1368
Size: 1256 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 1158 1368
Size: 1158 210
BbvCI CC'TCA_GC
Cuts at: 0 1274 1368
Size: 1274 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1159 1193 1230 1264 1368
Size: 1159 34 37 34 104
Fragments arranged by size:
1159 104 37 34 34
BclI T'GATC_A
Cuts at: 0 371 431 1368
Size: 371 60 937
Fragments arranged by size:
937 371 60
BglI GCCn_nnn'nGGC
Cuts at: 0 548 565 1300 1368
Size: 548 17 735 68
Fragments arranged by size:
735 548 68 17
Bme1580I G_kGCm'C
Cuts at: 0 1256 1368
Size: 1256 112
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 1339 1368
Size: 315 1024 29
Fragments arranged by size:
1024 315 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 237 1368
Size: 237 1131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 1368
Size: 818 550
Bpu10I CC'TnA_GC
Cuts at: 0 212 1274 1368
Size: 212 1062 94
Fragments arranged by size:
1062 212 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1368
Size: 697 671
BsaI GGTCTCn'nnnn_
Cuts at: 0 733 1276 1368
Size: 733 543 92
Fragments arranged by size:
733 543 92
BsaBI GATnn'nnATC
Cuts at: 0 436 1368
Size: 436 932
BsaHI Gr'CG_yC
Cuts at: 0 176 184 1368
Size: 176 8 1184
Fragments arranged by size:
1184 176 8
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 730 760 791 821 1368
Size: 280 30 420 30 31 30 547
Fragments arranged by size:
547 420 280 31 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 784 1368
Size: 784 584
BseYI C'CCAG_C
Cuts at: 0 60 1368
Size: 60 1308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1368
Size: 415 953
BsiHKAI G_wGCw'C
Cuts at: 0 446 1175 1368
Size: 446 729 193
Fragments arranged by size:
729 446 193
BsiWI C'GTAC_G
Cuts at: 0 327 1368
Size: 327 1041
Bsp1286I G_dGCh'C
Cuts at: 0 446 1175 1256 1368
Size: 446 729 81 112
Fragments arranged by size:
729 446 112 81
BspHI T'CATG_A
Cuts at: 0 95 248 527 1368
Size: 95 153 279 841
Fragments arranged by size:
841 279 153 95
BsrBI CCG'CTC
Cuts at: 0 216 1368
Size: 216 1152
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 1368
Size: 360 1008
BstYI r'GATC_y
Cuts at: 0 314 332 1368
Size: 314 18 1036
Fragments arranged by size:
1036 314 18
Bsu36I CC'TnA_GG
Cuts at: 0 20 1368
Size: 20 1348
BtgI C'CryG_G
Cuts at: 0 82 563 1368
Size: 82 481 805
Fragments arranged by size:
805 481 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 936 1368
Size: 936 432
BtsI GCAGTG_nn'
Cuts at: 0 155 374 1117 1368
Size: 155 219 743 251
Fragments arranged by size:
743 251 219 155
EaeI y'GGCC_r
Cuts at: 0 101 350 534 566 1368
Size: 101 249 184 32 802
Fragments arranged by size:
802 249 184 101 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 1368
Size: 818 550
EcoNI CCTnn'n_nnAGG
Cuts at: 0 388 1368
Size: 388 980
EcoO109I rG'GnC_Cy
Cuts at: 0 540 724 1368
Size: 540 184 644
Fragments arranged by size:
644 540 184
EcoRI G'AATT_C
Cuts at: 0 688 1368
Size: 688 680
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 90 122 791 823 928 960 1368
Size: 26 64 32 669 32 105 32 408
Fragments arranged by size:
669 408 105 64 32 32 32 26
HindIII A'AGCT_T
Cuts at: 0 715 1368
Size: 715 653
MfeI C'AATT_G
Cuts at: 0 1361 1368
Size: 1361 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 836 1241 1368
Size: 728 108 405 127
Fragments arranged by size:
728 405 127 108
MscI TGG'CCA
Cuts at: 0 352 536 568 1368
Size: 352 184 32 800
Fragments arranged by size:
800 352 184 32
MslI CAynn'nnrTG
Cuts at: 0 385 427 913 988 1368
Size: 385 42 486 75 380
Fragments arranged by size:
486 385 380 75 42
MspA1I CmG'CkG
Cuts at: 0 399 1045 1076 1273 1368
Size: 399 646 31 197 95
Fragments arranged by size:
646 399 197 95 31
NspI r_CATG'y
Cuts at: 0 135 1245 1368
Size: 135 1110 123
Fragments arranged by size:
1110 135 123
PciI A'CATG_T
Cuts at: 0 131 1368
Size: 131 1237
PpuMI rG'GwC_Cy
Cuts at: 0 724 1368
Size: 724 644
PshAI GACnn'nnGTC
Cuts at: 0 1162 1368
Size: 1162 206
PspOMI G'GGCC_C
Cuts at: 0 1252 1368
Size: 1252 116
PstI C_TGCA'G
Cuts at: 0 201 473 1368
Size: 201 272 895
Fragments arranged by size:
895 272 201
PvuII CAG'CTG
Cuts at: 0 399 1045 1273 1368
Size: 399 646 228 95
Fragments arranged by size:
646 399 228 95
SbfI CC_TGCA'GG
Cuts at: 0 473 1368
Size: 473 895
SfcI C'TryA_G
Cuts at: 0 197 469 1368
Size: 197 272 899
Fragments arranged by size:
899 272 197
SmaI CCC'GGG
Cuts at: 0 729 1368
Size: 729 639
SmlI C'TyrA_G
Cuts at: 0 712 1368
Size: 712 656
StyI C'CwwG_G
Cuts at: 0 149 1368
Size: 149 1219
XmaI C'CCGG_G
Cuts at: 0 727 1368
Size: 727 641
XmnI GAAnn'nnTTC
Cuts at: 0 91 1368
Size: 91 1277
Enzymes that do cut:
AclI AflIII AhdI AlfI AlwNI ApaI ApoI AseI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI
Bsp1286I BspHI BsrBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI Eco57MI EcoNI EcoO109I EcoRI Hin4I HindIII
MfeI MmeI MscI MslI MspA1I NspI PciI PpuMI
PshAI PspOMI PstI PvuII SbfI SfcI SmaI SmlI
StyI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AgeI
AleI AloI ApaLI AscI AsiSI AvrII BaeI BbeI
BciVI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaWI
BsiEI BsmI BsmBI BspEI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
ZraI