Restriction map for
pFN21AE4438
(Linear) (Six-base) MAPSORT of: KIEE4438.seq Check: 3005 from: 1 to: 1365
KIEE4438.orf
With 182 enzymes: SgfI *
March 22, 2012 18:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 306 1365
Size: 306 1059
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 289 1365
Size: 8 281 1076
Fragments arranged by size:
1076 281 8
AfeI AGC'GCT
Cuts at: 0 1114 1365
Size: 1114 251
AhdI GACnn_n'nnGTC
Cuts at: 0 681 1365
Size: 681 684
AleI CACnn'nnGTG
Cuts at: 0 25 1365
Size: 25 1340
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 1365
Size: 1027 32 306
Fragments arranged by size:
1027 306 32
ApaLI G'TGCA_C
Cuts at: 0 439 909 1365
Size: 439 470 456
Fragments arranged by size:
470 456 439
ApoI r'AATT_y
Cuts at: 0 298 655 1006 1365
Size: 298 357 351 359
Fragments arranged by size:
359 357 351 298
AvaI C'yCGr_G
Cuts at: 0 59 1365
Size: 59 1306
BanI G'GyrC_C
Cuts at: 0 714 1093 1365
Size: 714 379 272
Fragments arranged by size:
714 379 272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 411 445 1365
Size: 411 34 920
Fragments arranged by size:
920 411 34
Bme1580I G_kGCm'C
Cuts at: 0 443 719 913 1184 1365
Size: 443 276 194 271 181
Fragments arranged by size:
443 276 271 194 181
BmrI ACTGGGnnnn_n'
Cuts at: 0 687 1365
Size: 687 678
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 1365
Size: 597 768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1365
Size: 1039 326
Bpu10I CC'TnA_GC
Cuts at: 0 514 1365
Size: 514 851
BsaAI yAC'GTr
Cuts at: 0 387 726 1365
Size: 387 339 639
Fragments arranged by size:
639 387 339
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 829 859 1365
Size: 829 30 506
Fragments arranged by size:
829 506 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 187 775 1095 1321 1365
Size: 7 180 588 320 226 44
Fragments arranged by size:
588 320 226 180 44 7
BseYI C'CCAG_C
Cuts at: 0 30 398 642 707 718 1109 1365
Size: 30 368 244 65 11 391 256
Fragments arranged by size:
391 368 256 244 65 30 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 721 1365
Size: 678 43 644
Fragments arranged by size:
678 644 43
BsiHKAI G_wGCw'C
Cuts at: 0 443 527 913 1365
Size: 443 84 386 452
Fragments arranged by size:
452 443 386 84
Bsp1286I G_dGCh'C
Cuts at: 0 443 527 719 913 1184 1365
Size: 443 84 192 194 271 181
Fragments arranged by size:
443 271 194 192 181 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 306 1067 1365
Size: 306 761 298
Fragments arranged by size:
761 306 298
BsrBI CCG'CTC
Cuts at: 0 890 1164 1359 1365
Size: 890 274 195 6
Fragments arranged by size:
890 274 195 6
BssSI C'ACGA_G
Cuts at: 0 111 1365
Size: 111 1254
BstYI r'GATC_y
Cuts at: 0 176 617 1365
Size: 176 441 748
Fragments arranged by size:
748 441 176
BtgI C'CryG_G
Cuts at: 0 507 1365
Size: 507 858
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 533 1365
Size: 533 832
ClaI AT'CG_AT
Cuts at: 0 200 1365
Size: 200 1165
EaeI y'GGCC_r
Cuts at: 0 605 1365
Size: 605 760
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 133 306 1365
Size: 133 173 1059
Fragments arranged by size:
1059 173 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 289 1039 1365
Size: 8 281 750 326
Fragments arranged by size:
750 326 281 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1135 1365
Size: 1135 230
EcoO109I rG'GnC_Cy
Cuts at: 0 683 1365
Size: 683 682
EcoRI G'AATT_C
Cuts at: 0 655 1006 1365
Size: 655 351 359
Fragments arranged by size:
655 359 351
HaeII r_GCGC'y
Cuts at: 0 1116 1365
Size: 1116 249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 1365
Size: 667 32 666
Fragments arranged by size:
667 666 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1365
Size: 284 1081
MscI TGG'CCA
Cuts at: 0 607 1365
Size: 607 758
MslI CAynn'nnrTG
Cuts at: 0 25 385 700 1365
Size: 25 360 315 665
Fragments arranged by size:
665 360 315 25
MspA1I CmG'CkG
Cuts at: 0 236 402 711 1365
Size: 236 166 309 654
Fragments arranged by size:
654 309 236 166
NcoI C'CATG_G
Cuts at: 0 507 1365
Size: 507 858
NspI r_CATG'y
Cuts at: 0 900 1365
Size: 900 465
PflMI CCAn_nnn'nTGG
Cuts at: 0 47 1365
Size: 47 1318
PfoI T'CCnGG_A
Cuts at: 0 650 1365
Size: 650 715
PmlI CAC'GTG
Cuts at: 0 387 726 1365
Size: 387 339 639
Fragments arranged by size:
639 387 339
PpuMI rG'GwC_Cy
Cuts at: 0 683 1365
Size: 683 682
PshAI GACnn'nnGTC
Cuts at: 0 1069 1365
Size: 1069 296
PsiI TTA'TAA
Cuts at: 0 473 1365
Size: 473 892
PvuII CAG'CTG
Cuts at: 0 236 402 711 1365
Size: 236 166 309 654
Fragments arranged by size:
654 309 236 166
SanDI GG'GwC_CC
Cuts at: 0 683 1365
Size: 683 682
SfcI C'TryA_G
Cuts at: 0 870 1365
Size: 870 495
SmaI CCC'GGG
Cuts at: 0 61 1365
Size: 61 1304
StuI AGG'CCT
Cuts at: 0 734 1031 1365
Size: 734 297 334
Fragments arranged by size:
734 334 297
StyI C'CwwG_G
Cuts at: 0 507 1365
Size: 507 858
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 83 1224 1365
Size: 83 1141 141
Fragments arranged by size:
1141 141 83
XbaI T'CTAG_A
Cuts at: 0 975 1365
Size: 975 390
XmaI C'CCGG_G
Cuts at: 0 59 1365
Size: 59 1306
XmnI GAAnn'nnTTC
Cuts at: 0 592 865 1365
Size: 592 273 500
Fragments arranged by size:
592 500 273
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI AloI ApaLI ApoI
AvaI BanI BcgI Bme1580I BmrI BplI BpmI Bpu10I
BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BssSI BstYI BtgI BtgZI ClaI EaeI EciI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MmeI MscI
MslI MspA1I NcoI NspI PflMI PfoI PmlI PpuMI
PshAI PsiI PvuII SanDI SfcI SmaI StuI StyI
TaqII XbaI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AlfI
AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PpiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XcmI XhoI ZraI