Restriction map for
pFN21AE4435
(Linear) (Six-base) MAPSORT of: KIEE4435.seq Check: 6299 from: 1 to: 1356
KIEE4435.orf
With 182 enzymes: SgfI *
March 22, 2012 18:01 ..
AatII G_ACGT'C
Cuts at: 0 994 1356
Size: 994 362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1356
Size: 403 953
AflIII A'CryG_T
Cuts at: 0 1126 1356
Size: 1126 230
AlwNI CAG_nnn'CTG
Cuts at: 0 1191 1356
Size: 1191 165
AvaI C'yCGr_G
Cuts at: 0 250 1356
Size: 250 1106
BanI G'GyrC_C
Cuts at: 0 63 107 1356
Size: 63 44 1249
Fragments arranged by size:
1249 63 44
BanII G_rGCy'C
Cuts at: 0 257 452 1344 1356
Size: 257 195 892 12
Fragments arranged by size:
892 257 195 12
BbeI G_GCGC'C
Cuts at: 0 67 111 1356
Size: 67 44 1245
Fragments arranged by size:
1245 67 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 432 576 890 1024 1356
Size: 432 144 314 134 332
Fragments arranged by size:
432 332 314 144 134
BbvCI CC'TCA_GC
Cuts at: 0 1031 1264 1356
Size: 1031 233 92
Fragments arranged by size:
1031 233 92
BglII A'GATC_T
Cuts at: 0 803 845 1356
Size: 803 42 511
Fragments arranged by size:
803 511 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 699 1356
Size: 699 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1213 1356
Size: 1156 57 143
Fragments arranged by size:
1156 143 57
Bpu10I CC'TnA_GC
Cuts at: 0 884 1031 1264 1356
Size: 884 147 233 92
Fragments arranged by size:
884 233 147 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1356
Size: 877 479
BsaAI yAC'GTr
Cuts at: 0 222 1129 1356
Size: 222 907 227
Fragments arranged by size:
907 227 222
BsaHI Gr'CG_yC
Cuts at: 0 64 108 991 1356
Size: 64 44 883 365
Fragments arranged by size:
883 365 64 44
BsaWI w'CCGG_w
Cuts at: 0 416 1356
Size: 416 940
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1186 1216 1356
Size: 1186 30 140
Fragments arranged by size:
1186 140 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 964 1356
Size: 284 680 392
Fragments arranged by size:
680 392 284
BsiEI CG_ry'CG
Cuts at: 0 55 1356
Size: 55 1301
BsiHKAI G_wGCw'C
Cuts at: 0 748 860 1073 1142 1289 1356
Size: 748 112 213 69 147 67
Fragments arranged by size:
748 213 147 112 69 67
BsmI GAATG_Cn'
Cuts at: 0 1253 1356
Size: 1253 103
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1204 1356
Size: 1204 152
Bsp1286I G_dGCh'C
Cuts at: 0 257 452 748 860 1073 1142 1289 1344
Size: 257 195 296 112 213 69 147 55
Cuts at: 1344 1356
Size: 12
Fragments arranged by size:
296 257 213 195 147 112 69 55
12
BspEI T'CCGG_A
Cuts at: 0 416 1356
Size: 416 940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 1356
Size: 20 1336
BsrBI CCG'CTC
Cuts at: 0 1039 1356
Size: 1039 317
BsrDI GCAATG_nn'
Cuts at: 0 1275 1356
Size: 1275 81
BsrFI r'CCGG_y
Cuts at: 0 4 54 66 565 860 1082 1356
Size: 4 50 12 499 295 222 274
Fragments arranged by size:
499 295 274 222 50 12 4
BsrGI T'GTAC_A
Cuts at: 0 626 662 737 1356
Size: 626 36 75 619
Fragments arranged by size:
626 619 75 36
BstEII G'GTnAC_C
Cuts at: 0 285 1356
Size: 285 1071
BstXI CCAn_nnnn'nTGG
Cuts at: 0 970 1242 1356
Size: 970 272 114
Fragments arranged by size:
970 272 114
BstYI r'GATC_y
Cuts at: 0 803 845 1356
Size: 803 42 511
Fragments arranged by size:
803 511 42
Bsu36I CC'TnA_GG
Cuts at: 0 42 1356
Size: 42 1314
BtgI C'CryG_G
Cuts at: 0 103 146 522 1301 1356
Size: 103 43 376 779 55
Fragments arranged by size:
779 376 103 55 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1089 1356
Size: 1089 267
BtsI GCAGTG_nn'
Cuts at: 0 609 1356
Size: 609 747
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 531 566 1356
Size: 531 35 790
Fragments arranged by size:
790 531 35
EaeI y'GGCC_r
Cuts at: 0 2 52 305 730 1356
Size: 2 50 253 425 626
Fragments arranged by size:
626 425 253 50 2
EagI C'GGCC_G
Cuts at: 0 52 1356
Size: 52 1304
EarI CTCTTCn'nnn_
Cuts at: 0 365 1356
Size: 365 991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1156 1213 1356
Size: 403 753 57 143
Fragments arranged by size:
753 403 143 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 961 1356
Size: 961 395
EcoO109I rG'GnC_Cy
Cuts at: 0 888 1239 1356
Size: 888 351 117
Fragments arranged by size:
888 351 117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 810 846 1356
Size: 810 36 510
Fragments arranged by size:
810 510 36
FseI GG_CCGG'CC
Cuts at: 0 58 1086 1356
Size: 58 1028 270
Fragments arranged by size:
1028 270 58
FspI TGC'GCA
Cuts at: 0 695 1356
Size: 695 661
HaeII r_GCGC'y
Cuts at: 0 67 111 1356
Size: 67 44 1245
Fragments arranged by size:
1245 67 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 931 963 1356
Size: 931 32 393
Fragments arranged by size:
931 393 32
KasI G'GCGC_C
Cuts at: 0 63 107 1356
Size: 63 44 1249
Fragments arranged by size:
1249 63 44
MscI TGG'CCA
Cuts at: 0 307 1356
Size: 307 1049
MslI CAynn'nnrTG
Cuts at: 0 521 658 1356
Size: 521 137 698
Fragments arranged by size:
698 521 137
MspA1I CmG'CkG
Cuts at: 0 105 701 735 1356
Size: 105 596 34 621
Fragments arranged by size:
621 596 105 34
NaeI GCC'GGC
Cuts at: 0 6 56 68 567 1084 1356
Size: 6 50 12 499 517 272
Fragments arranged by size:
517 499 272 50 12 6
NarI GG'CG_CC
Cuts at: 0 64 108 1356
Size: 64 44 1248
Fragments arranged by size:
1248 64 44
NcoI C'CATG_G
Cuts at: 0 522 1356
Size: 522 834
NgoMIV G'CCGG_C
Cuts at: 0 4 54 66 565 1082 1356
Size: 4 50 12 499 517 274
Fragments arranged by size:
517 499 274 50 12 4
NspI r_CATG'y
Cuts at: 0 663 1356
Size: 663 693
PasI CC'CwG_GG
Cuts at: 0 963 1344 1356
Size: 963 381 12
Fragments arranged by size:
963 381 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 598 1191 1356
Size: 598 593 165
Fragments arranged by size:
598 593 165
PfoI T'CCnGG_A
Cuts at: 0 443 797 1356
Size: 443 354 559
Fragments arranged by size:
559 443 354
PmlI CAC'GTG
Cuts at: 0 222 1129 1356
Size: 222 907 227
Fragments arranged by size:
907 227 222
PpuMI rG'GwC_Cy
Cuts at: 0 888 1239 1356
Size: 888 351 117
Fragments arranged by size:
888 351 117
PshAI GACnn'nnGTC
Cuts at: 0 994 1356
Size: 994 362
PstI C_TGCA'G
Cuts at: 0 37 1356
Size: 37 1319
PvuII CAG'CTG
Cuts at: 0 701 1356
Size: 701 655
SacII CC_GC'GG
Cuts at: 0 106 1356
Size: 106 1250
SexAI A'CCwGG_T
Cuts at: 0 281 1356
Size: 281 1075
SfcI C'TryA_G
Cuts at: 0 33 1356
Size: 33 1323
SfoI GGC'GCC
Cuts at: 0 65 109 1356
Size: 65 44 1247
Fragments arranged by size:
1247 65 44
SgrAI Cr'CCGG_yG
Cuts at: 0 66 1356
Size: 66 1290
SmlI C'TyrA_G
Cuts at: 0 892 1356
Size: 892 464
StyI C'CwwG_G
Cuts at: 0 522 978 1235 1356
Size: 522 456 257 121
Fragments arranged by size:
522 456 257 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 841 1356
Size: 325 516 515
Fragments arranged by size:
516 515 325
TatI w'GTAC_w
Cuts at: 0 317 533 626 662 737 1356
Size: 317 216 93 36 75 619
Fragments arranged by size:
619 317 216 93 75 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 464 496 1186 1218 1356
Size: 464 32 690 32 138
Fragments arranged by size:
690 464 138 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 283 1356
Size: 283 1073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 549 1356
Size: 549 807
ZraI GAC'GTC
Cuts at: 0 992 1356
Size: 992 364
Enzymes that do cut:
AatII AcuI AflIII AlwNI AvaI BanI BanII BbeI
BbsI BbvCI BglII BplI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI
EagI EarI Eco57MI EcoNI EcoO109I FalI FseI FspI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI
PshAI PstI PvuII SacII SexAI SfcI SfoI SgrAI
SmlI StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI BsaI BsaBI
BseRI BseYI BsiWI BspHI BssHII BssSI BstAPI BstBI
BstZ17I ClaI DraI DraIII DrdI EciI EcoICRI EcoRI
EcoRV FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SfiI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI XmaI XmnI