Restriction map for
pFN21AE4431
(Linear) (Six-base) MAPSORT of: KIEE4431.seq Check: 5104 from: 1 to: 1350
KIEE4431.orf
With 182 enzymes: SgfI *
March 22, 2012 18:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 27 1350
Size: 27 1323
AatII G_ACGT'C
Cuts at: 0 19 1350
Size: 19 1331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 760 811 846 1350
Size: 367 393 51 35 504
Fragments arranged by size:
504 393 367 51 35
AfeI AGC'GCT
Cuts at: 0 58 1350
Size: 58 1292
AflII C'TTAA_G
Cuts at: 0 1306 1350
Size: 1306 44
AflIII A'CryG_T
Cuts at: 0 1162 1350
Size: 1162 188
AhdI GACnn_n'nnGTC
Cuts at: 0 349 375 1350
Size: 349 26 975
Fragments arranged by size:
975 349 26
AlwNI CAG_nnn'CTG
Cuts at: 0 72 591 720 1350
Size: 72 519 129 630
Fragments arranged by size:
630 519 129 72
ApoI r'AATT_y
Cuts at: 0 703 970 1350
Size: 703 267 380
Fragments arranged by size:
703 380 267
AseI AT'TA_AT
Cuts at: 0 908 1350
Size: 908 442
AvaI C'yCGr_G
Cuts at: 0 634 1350
Size: 634 716
BanI G'GyrC_C
Cuts at: 0 319 1350
Size: 319 1031
BbsI GAAGACnn'nnnn_
Cuts at: 0 348 602 1165 1217 1350
Size: 348 254 563 52 133
Fragments arranged by size:
563 348 254 133 52
BbvCI CC'TCA_GC
Cuts at: 0 334 650 1350
Size: 334 316 700
Fragments arranged by size:
700 334 316
BglII A'GATC_T
Cuts at: 0 500 1350
Size: 500 850
BmgBI CAC'GTC
Cuts at: 0 600 1350
Size: 600 750
BmrI ACTGGGnnnn_n'
Cuts at: 0 365 875 1245 1350
Size: 365 510 370 105
Fragments arranged by size:
510 370 365 105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 204 1350
Size: 204 1146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1036 1350
Size: 742 294 314
Fragments arranged by size:
742 314 294
Bpu10I CC'TnA_GC
Cuts at: 0 334 650 1350
Size: 334 316 700
Fragments arranged by size:
700 334 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 1350
Size: 1270 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 160 1350
Size: 160 1190
BsaBI GATnn'nnATC
Cuts at: 0 395 1350
Size: 395 955
BsaHI Gr'CG_yC
Cuts at: 0 16 1350
Size: 16 1334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1033 1063 1350
Size: 1033 30 287
Fragments arranged by size:
1033 287 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 206 211 583 586 1279 1350
Size: 206 5 372 3 693 71
Fragments arranged by size:
693 372 206 71 5 3
BseYI C'CCAG_C
Cuts at: 0 252 867 1350
Size: 252 615 483
Fragments arranged by size:
615 483 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 1350
Size: 1147 203
BsmI GAATG_Cn'
Cuts at: 0 421 1350
Size: 421 929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 27 1350
Size: 27 1323
BsrDI GCAATG_nn'
Cuts at: 0 1109 1350
Size: 1109 241
BssSI C'ACGA_G
Cuts at: 0 84 1350
Size: 84 1266
BstXI CCAn_nnnn'nTGG
Cuts at: 0 960 1350
Size: 960 390
BstYI r'GATC_y
Cuts at: 0 95 500 1350
Size: 95 405 850
Fragments arranged by size:
850 405 95
BtgI C'CryG_G
Cuts at: 0 323 890 1350
Size: 323 567 460
Fragments arranged by size:
567 460 323
BtsI GCAGTG_nn'
Cuts at: 0 1230 1276 1350
Size: 1230 46 74
Fragments arranged by size:
1230 74 46
DraI TTT'AAA
Cuts at: 0 708 1350
Size: 708 642
DraIII CAC_nnn'GTG
Cuts at: 0 409 1350
Size: 409 941
EaeI y'GGCC_r
Cuts at: 0 950 1350
Size: 950 400
EarI CTCTTCn'nnn_
Cuts at: 0 173 736 1136 1350
Size: 173 563 400 214
Fragments arranged by size:
563 400 214 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 742 760 811 846 1036 1350
Size: 367 375 18 51 35 190 314
Fragments arranged by size:
375 367 314 190 51 35 18
EcoO109I rG'GnC_Cy
Cuts at: 0 360 1350
Size: 360 990
HaeII r_GCGC'y
Cuts at: 0 60 1350
Size: 60 1290
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 335 361 367 393 651 683
Size: 217 32 86 26 6 26 258 32
Cuts at: 683 1266 1298 1350
Size: 583 32 52
Fragments arranged by size:
583 258 217 86 52 32 32 32
26 26 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 1350
Size: 44 1306
MscI TGG'CCA
Cuts at: 0 952 1350
Size: 952 398
MslI CAynn'nnrTG
Cuts at: 0 37 1350
Size: 37 1313
MspA1I CmG'CkG
Cuts at: 0 441 720 780 1350
Size: 441 279 60 570
Fragments arranged by size:
570 441 279 60
NcoI C'CATG_G
Cuts at: 0 323 1350
Size: 323 1027
PflMI CCAn_nnn'nTGG
Cuts at: 0 591 948 1104 1350
Size: 591 357 156 246
Fragments arranged by size:
591 357 246 156
PfoI T'CCnGG_A
Cuts at: 0 98 116 584 1115 1350
Size: 98 18 468 531 235
Fragments arranged by size:
531 468 235 98 18
PpuMI rG'GwC_Cy
Cuts at: 0 360 1350
Size: 360 990
PsiI TTA'TAA
Cuts at: 0 1343 1350
Size: 1343 7
PstI C_TGCA'G
Cuts at: 0 64 1350
Size: 64 1286
PvuII CAG'CTG
Cuts at: 0 441 720 780 1350
Size: 441 279 60 570
Fragments arranged by size:
570 441 279 60
SfcI C'TryA_G
Cuts at: 0 60 1179 1350
Size: 60 1119 171
Fragments arranged by size:
1119 171 60
SmlI C'TyrA_G
Cuts at: 0 634 1285 1306 1350
Size: 634 651 21 44
Fragments arranged by size:
651 634 44 21
StyI C'CwwG_G
Cuts at: 0 323 1350
Size: 323 1027
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 416 1314 1350
Size: 416 898 36
Fragments arranged by size:
898 416 36
Tth111I GACn'n_nGTC
Cuts at: 0 106 1350
Size: 106 1244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 888 1350
Size: 888 462
XhoI C'TCGA_G
Cuts at: 0 634 1350
Size: 634 716
XmnI GAAnn'nnTTC
Cuts at: 0 1005 1350
Size: 1005 345
ZraI GAC'GTC
Cuts at: 0 17 1350
Size: 17 1333
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AhdI AlwNI
ApoI AseI AvaI BanI BbsI BbvCI BglII BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsmI BspMI BsrDI BssSI
BstXI BstYI BtgI BtsI DraI DraIII EaeI EarI
Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I
NcoI PflMI PfoI PpuMI PsiI PstI PvuII SfcI
SmlI StyI TaqII Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AgeI AleI AlfI AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BanII BbeI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmtI
BsaAI BsaWI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI XbaI XmaI