Restriction map for
pFN21AE4430
(Linear) (Six-base) MAPSORT of: KIEE4430.seq Check: 6451 from: 1 to: 1344
KIEE4430.orf
With 182 enzymes: SgfI *
March 22, 2012 18:00 ..
AatII G_ACGT'C
Cuts at: 0 971 1344
Size: 971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 1232 1344
Size: 570 662 112
Fragments arranged by size:
662 570 112
AleI CACnn'nnGTG
Cuts at: 0 626 1344
Size: 626 718
AlwNI CAG_nnn'CTG
Cuts at: 0 109 712 910 964 1344
Size: 109 603 198 54 380
Fragments arranged by size:
603 380 198 109 54
ApaI G_GGCC'C
Cuts at: 0 49 74 1344
Size: 49 25 1270
Fragments arranged by size:
1270 49 25
ApaLI G'TGCA_C
Cuts at: 0 562 1344
Size: 562 782
ApoI r'AATT_y
Cuts at: 0 535 1144 1344
Size: 535 609 200
Fragments arranged by size:
609 535 200
AvaI C'yCGr_G
Cuts at: 0 31 164 1344
Size: 31 133 1180
Fragments arranged by size:
1180 133 31
AvrII C'CTAG_G
Cuts at: 0 1102 1344
Size: 1102 242
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 276 309 1344
Size: 276 33 1035
Fragments arranged by size:
1035 276 33
BamHI G'GATC_C
Cuts at: 0 655 1344
Size: 655 689
BanII G_rGCy'C
Cuts at: 0 49 74 230 445 519 644 926 1048
Size: 49 25 156 215 74 125 282 122
Cuts at: 1048 1316 1344
Size: 268 28
Fragments arranged by size:
282 268 215 156 125 122 74 49
28 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1064 1344
Size: 1064 280
BclI T'GATC_A
Cuts at: 0 113 464 1344
Size: 113 351 880
Fragments arranged by size:
880 351 113
BglI GCCn_nnn'nGGC
Cuts at: 0 311 1333 1344
Size: 311 1022 11
Fragments arranged by size:
1022 311 11
BlpI GC'TnA_GC
Cuts at: 0 98 518 1310 1344
Size: 98 420 792 34
Fragments arranged by size:
792 420 98 34
Bme1580I G_kGCm'C
Cuts at: 0 49 74 566 1344
Size: 49 25 492 778
Fragments arranged by size:
778 492 49 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 320 620 780 1310 1344
Size: 320 300 160 530 34
Fragments arranged by size:
530 320 300 160 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 1344
Size: 123 1221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 244 358 658 887 1080 1289 1344
Size: 178 66 114 300 229 193 209 55
Fragments arranged by size:
300 229 209 193 178 114 66 55
BsaI GGTCTCn'nnnn_
Cuts at: 0 804 1344
Size: 804 540
BsaHI Gr'CG_yC
Cuts at: 0 849 968 1344
Size: 849 119 376
Fragments arranged by size:
849 376 119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 586 616 1263 1293 1344
Size: 586 30 647 30 51
Fragments arranged by size:
647 586 51 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 811 814 823 826 960 1112 1271 1344
Size: 811 3 9 3 134 152 159 73
Fragments arranged by size:
811 159 152 134 73 9 3 3
BseYI C'CCAG_C
Cuts at: 0 119 195 957 1344
Size: 119 76 762 387
Fragments arranged by size:
762 387 119 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 1344
Size: 1228 116
BsiHKAI G_wGCw'C
Cuts at: 0 512 519 566 644 1344
Size: 512 7 47 78 700
Fragments arranged by size:
700 512 78 47 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 699 1344
Size: 699 645
Bsp1286I G_dGCh'C
Cuts at: 0 49 74 230 445 512 519 566 644
Size: 49 25 156 215 67 7 47 78
Cuts at: 644 926 1048 1316 1344
Size: 282 122 268 28
Fragments arranged by size:
282 268 215 156 122 78 67 49
47 28 25 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 620 1344
Size: 620 724
BsrBI CCG'CTC
Cuts at: 0 474 1344
Size: 474 870
BstXI CCAn_nnnn'nTGG
Cuts at: 0 203 1344
Size: 203 1141
BstYI r'GATC_y
Cuts at: 0 655 1344
Size: 655 689
Bsu36I CC'TnA_GG
Cuts at: 0 405 713 1013 1344
Size: 405 308 300 331
Fragments arranged by size:
405 331 308 300
BtsI GCAGTG_nn'
Cuts at: 0 1076 1344
Size: 1076 268
EaeI y'GGCC_r
Cuts at: 0 1334 1344
Size: 1334 10
EarI CTCTTCn'nnn_
Cuts at: 0 832 1169 1344
Size: 832 337 175
Fragments arranged by size:
832 337 175
EcoICRI GAG'CTC
Cuts at: 0 517 642 1344
Size: 517 125 702
Fragments arranged by size:
702 517 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 244 358 570 658 887 1080 1232
Size: 178 66 114 212 88 229 193 152
Cuts at: 1232 1289 1344
Size: 57 55
Fragments arranged by size:
229 212 193 178 152 114 88 66
57 55
EcoO109I rG'GnC_Cy
Cuts at: 0 46 70 71 152 572 667 674 709
Size: 46 24 1 81 420 95 7 35
Cuts at: 709 936 1298 1344
Size: 227 362 46
Fragments arranged by size:
420 362 227 95 81 46 46 35
24 7 1
EcoRI G'AATT_C
Cuts at: 0 535 1144 1344
Size: 535 609 200
Fragments arranged by size:
609 535 200
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 1344
Size: 236 32 1076
Fragments arranged by size:
1076 236 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 864 918 1344
Size: 864 54 426
Fragments arranged by size:
864 426 54
MscI TGG'CCA
Cuts at: 0 1336 1344
Size: 1336 8
MslI CAynn'nnrTG
Cuts at: 0 626 1344
Size: 626 718
MspA1I CmG'CkG
Cuts at: 0 119 290 703 961 1309 1344
Size: 119 171 413 258 348 35
Fragments arranged by size:
413 348 258 171 119 35
NspI r_CATG'y
Cuts at: 0 63 1344
Size: 63 1281
PasI CC'CwG_GG
Cuts at: 0 203 1344
Size: 203 1141
PflMI CCAn_nnn'nTGG
Cuts at: 0 92 1138 1344
Size: 92 1046 206
Fragments arranged by size:
1046 206 92
PfoI T'CCnGG_A
Cuts at: 0 1148 1344
Size: 1148 196
PpuMI rG'GwC_Cy
Cuts at: 0 152 572 667 1344
Size: 152 420 95 677
Fragments arranged by size:
677 420 152 95
PspOMI G'GGCC_C
Cuts at: 0 45 70 1344
Size: 45 25 1274
Fragments arranged by size:
1274 45 25
PstI C_TGCA'G
Cuts at: 0 708 1344
Size: 708 636
PvuII CAG'CTG
Cuts at: 0 119 703 961 1309 1344
Size: 119 584 258 348 35
Fragments arranged by size:
584 348 258 119 35
SacI G_AGCT'C
Cuts at: 0 519 644 1344
Size: 519 125 700
Fragments arranged by size:
700 519 125
SanDI GG'GwC_CC
Cuts at: 0 152 667 1344
Size: 152 515 677
Fragments arranged by size:
677 515 152
SfcI C'TryA_G
Cuts at: 0 308 360 576 704 734 940 1344
Size: 308 52 216 128 30 206 404
Fragments arranged by size:
404 308 216 206 128 52 30
StyI C'CwwG_G
Cuts at: 0 659 926 1082 1102 1344
Size: 659 267 156 20 242
Fragments arranged by size:
659 267 242 156 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 696 1344
Size: 70 626 648
Fragments arranged by size:
648 626 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 401 433 586 618 1344
Size: 401 32 153 32 726
Fragments arranged by size:
726 401 153 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 633 1344
Size: 333 300 711
Fragments arranged by size:
711 333 300
ZraI GAC'GTC
Cuts at: 0 969 1344
Size: 969 375
Enzymes that do cut:
AatII AcuI AleI AlwNI ApaI ApaLI ApoI AvaI
AvrII BaeI BamHI BanII BbsI BclI BglI BlpI
Bme1580I BmrI BplI BpmI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BstXI
BstYI Bsu36I BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I MmeI MscI MslI MspA1I NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SfcI StyI TaqII TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AscI AseI AsiSI BanI BbeI
BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI