Restriction map for
pFN21AE4429
(Linear) (Six-base) MAPSORT of: KIEE4429.seq Check: 4524 from: 1 to: 1335
KIEE4429.orf
With 182 enzymes: SgfI *
March 22, 2012 18:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1307 1335
Size: 1307 28
Acc65I G'GTAC_C
Cuts at: 0 1247 1335
Size: 1247 88
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 874 1335
Size: 554 320 461
Fragments arranged by size:
554 461 320
AflIII A'CryG_T
Cuts at: 0 995 1335
Size: 995 340
AleI CACnn'nnGTG
Cuts at: 0 739 1335
Size: 739 596
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 484 516 1335
Size: 484 32 819
Fragments arranged by size:
819 484 32
AlwNI CAG_nnn'CTG
Cuts at: 0 649 943 957 1009 1335
Size: 649 294 14 52 326
Fragments arranged by size:
649 326 294 52 14
ApaI G_GGCC'C
Cuts at: 0 1329 1335
Size: 1329 6
ApoI r'AATT_y
Cuts at: 0 1285 1335
Size: 1285 50
AvaI C'yCGr_G
Cuts at: 0 26 910 1328 1335
Size: 26 884 418 7
Fragments arranged by size:
884 418 26 7
AvrII C'CTAG_G
Cuts at: 0 330 1335
Size: 330 1005
BanI G'GyrC_C
Cuts at: 0 1247 1335
Size: 1247 88
BanII G_rGCy'C
Cuts at: 0 70 884 1329 1335
Size: 70 814 445 6
Fragments arranged by size:
814 445 70 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 862 977 1335
Size: 862 115 358
Fragments arranged by size:
862 358 115
BglI GCCn_nnn'nGGC
Cuts at: 0 1157 1335
Size: 1157 178
BglII A'GATC_T
Cuts at: 0 752 1224 1335
Size: 752 472 111
Fragments arranged by size:
752 472 111
BlpI GC'TnA_GC
Cuts at: 0 433 1335
Size: 433 902
Bme1580I G_kGCm'C
Cuts at: 0 1329 1335
Size: 1329 6
BmgBI CAC'GTC
Cuts at: 0 996 1335
Size: 996 339
Bpu10I CC'TnA_GC
Cuts at: 0 698 1335
Size: 698 637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1213 1335
Size: 1156 57 122
Fragments arranged by size:
1156 122 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 487 1029 1335
Size: 487 542 306
Fragments arranged by size:
542 487 306
BsaWI w'CCGG_w
Cuts at: 0 595 1335
Size: 595 740
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 484 514 532 562 694 724 871 901
Size: 484 30 18 30 132 30 147 30
Cuts at: 901 1335
Size: 434
Fragments arranged by size:
484 434 147 132 30 30 30 30
18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 60 87 553 1335
Size: 60 27 466 782
Fragments arranged by size:
782 466 60 27
BseYI C'CCAG_C
Cuts at: 0 676 1320 1335
Size: 676 644 15
Fragments arranged by size:
676 644 15
BsiHKAI G_wGCw'C
Cuts at: 0 603 884 1172 1335
Size: 603 281 288 163
Fragments arranged by size:
603 288 281 163
BsmI GAATG_Cn'
Cuts at: 0 397 1335
Size: 397 938
BsmBI CGTCTCn'nnnn_
Cuts at: 0 474 1335
Size: 474 861
Bsp1286I G_dGCh'C
Cuts at: 0 70 603 884 1172 1329 1335
Size: 70 533 281 288 157 6
Fragments arranged by size:
533 288 281 157 70 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 188 464 1307 1335
Size: 188 276 843 28
Fragments arranged by size:
843 276 188 28
BsrBI CCG'CTC
Cuts at: 0 705 1062 1335
Size: 705 357 273
Fragments arranged by size:
705 357 273
BsrGI T'GTAC_A
Cuts at: 0 812 1335
Size: 812 523
BssSI C'ACGA_G
Cuts at: 0 883 1335
Size: 883 452
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1009 1335
Size: 1009 326
BstXI CCAn_nnnn'nTGG
Cuts at: 0 741 1335
Size: 741 594
BstYI r'GATC_y
Cuts at: 0 752 953 1224 1335
Size: 752 201 271 111
Fragments arranged by size:
752 271 201 111
BtgI C'CryG_G
Cuts at: 0 53 576 734 1335
Size: 53 523 158 601
Fragments arranged by size:
601 523 158 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 558 1335
Size: 558 777
BtsI GCAGTG_nn'
Cuts at: 0 746 1335
Size: 746 589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1263 1298 1335
Size: 1263 35 37
Fragments arranged by size:
1263 37 35
DraI TTT'AAA
Cuts at: 0 273 1335
Size: 273 1062
DraIII CAC_nnn'GTG
Cuts at: 0 1108 1335
Size: 1108 227
DrdI GACnn_nn'nnGTC
Cuts at: 0 295 387 514 1335
Size: 295 92 127 821
Fragments arranged by size:
821 295 127 92
EaeI y'GGCC_r
Cuts at: 0 1178 1271 1335
Size: 1178 93 64
Fragments arranged by size:
1178 93 64
EarI CTCTTCn'nnn_
Cuts at: 0 169 190 530 761 1335
Size: 169 21 340 231 574
Fragments arranged by size:
574 340 231 169 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 101 136 706 1335
Size: 101 35 570 629
Fragments arranged by size:
629 570 101 35
EcoICRI GAG'CTC
Cuts at: 0 882 1335
Size: 882 453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 874 1335
Size: 554 320 461
Fragments arranged by size:
554 461 320
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1335
Size: 523 812
EcoO109I rG'GnC_Cy
Cuts at: 0 1325 1335
Size: 1325 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 1295 1327 1335
Size: 643 32 620 32 8
Fragments arranged by size:
643 620 32 32 8
KpnI G_GTAC'C
Cuts at: 0 1251 1335
Size: 1251 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1335
Size: 470 865
MslI CAynn'nnrTG
Cuts at: 0 739 1130 1335
Size: 739 391 205
Fragments arranged by size:
739 391 205
PasI CC'CwG_GG
Cuts at: 0 14 63 1153 1335
Size: 14 49 1090 182
Fragments arranged by size:
1090 182 49 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 756 1335
Size: 756 579
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 484 516 601 633 1335
Size: 484 32 85 32 702
Fragments arranged by size:
702 484 85 32 32
PspOMI G'GGCC_C
Cuts at: 0 1325 1335
Size: 1325 10
PstI C_TGCA'G
Cuts at: 0 350 908 962 1005 1335
Size: 350 558 54 43 330
Fragments arranged by size:
558 350 330 54 43
SacI G_AGCT'C
Cuts at: 0 884 1335
Size: 884 451
SapI GCTCTTCn'nnn_
Cuts at: 0 190 1335
Size: 190 1145
SexAI A'CCwGG_T
Cuts at: 0 496 1335
Size: 496 839
SfcI C'TryA_G
Cuts at: 0 346 904 958 1001 1335
Size: 346 558 54 43 334
Fragments arranged by size:
558 346 334 54 43
SmaI CCC'GGG
Cuts at: 0 1330 1335
Size: 1330 5
SmlI C'TyrA_G
Cuts at: 0 1171 1228 1335
Size: 1171 57 107
Fragments arranged by size:
1171 107 57
SrfI GCCC'GGGC
Cuts at: 0 1330 1335
Size: 1330 5
StyI C'CwwG_G
Cuts at: 0 330 1030 1335
Size: 330 700 305
Fragments arranged by size:
700 330 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 354 1088 1335
Size: 354 734 247
Fragments arranged by size:
734 354 247
TatI w'GTAC_w
Cuts at: 0 263 710 812 1335
Size: 263 447 102 523
Fragments arranged by size:
523 447 263 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 741 1335
Size: 741 594
XmaI C'CCGG_G
Cuts at: 0 1328 1335
Size: 1328 7
XmnI GAAnn'nnTTC
Cuts at: 0 688 859 1335
Size: 688 171 476
Fragments arranged by size:
688 476 171
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AloI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbsI BglI BglII
BlpI Bme1580I BmgBI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I KpnI MmeI MslI PasI
PflMI PpiI PspOMI PstI SacI SapI SexAI SfcI
SmaI SmlI SrfI StyI TaqII TatI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BmrI BmtI BplI BpmI
BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BspEI BspHI
BsrDI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I ClaI
EagI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I
XbaI XhoI ZraI