Restriction map for
pFN21AE4427
(Linear) (Six-base) MAPSORT of: KIEE4427.seq Check: 9127 from: 1 to: 1332
KIEE4427.orf
With 182 enzymes: SgfI *
March 22, 2012 17:59 ..
AatII G_ACGT'C
Cuts at: 0 466 1332
Size: 466 866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 631 1332
Size: 543 88 701
Fragments arranged by size:
701 543 88
AfeI AGC'GCT
Cuts at: 0 82 1332
Size: 82 1250
AleI CACnn'nnGTG
Cuts at: 0 928 1332
Size: 928 404
ApaI G_GGCC'C
Cuts at: 0 621 812 1332
Size: 621 191 520
Fragments arranged by size:
621 520 191
AvaI C'yCGr_G
Cuts at: 0 167 1332
Size: 167 1165
BamHI G'GATC_C
Cuts at: 0 204 1332
Size: 204 1128
BanI G'GyrC_C
Cuts at: 0 545 1332
Size: 545 787
BanII G_rGCy'C
Cuts at: 0 166 305 371 621 812 1332
Size: 166 139 66 250 191 520
Fragments arranged by size:
520 250 191 166 139 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 381 549 1332
Size: 381 168 783
Fragments arranged by size:
783 381 168
BbvCI CC'TCA_GC
Cuts at: 0 813 1332
Size: 813 519
BclI T'GATC_A
Cuts at: 0 1041 1332
Size: 1041 291
BfrBI ATG'CAT
Cuts at: 0 702 1332
Size: 702 630
Bme1580I G_kGCm'C
Cuts at: 0 548 621 812 1332
Size: 548 73 191 520
Fragments arranged by size:
548 520 191 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 1071 1332
Size: 1071 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1063 1332
Size: 266 797 269
Fragments arranged by size:
797 269 266
Bpu10I CC'TnA_GC
Cuts at: 0 35 84 813 1332
Size: 35 49 729 519
Fragments arranged by size:
729 519 49 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 19 1332
Size: 19 1313
BsaBI GATnn'nnATC
Cuts at: 0 1319 1332
Size: 1319 13
BsaHI Gr'CG_yC
Cuts at: 0 463 1332
Size: 463 869
BsaWI w'CCGG_w
Cuts at: 0 1049 1332
Size: 1049 283
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 472 1332
Size: 109 363 860
Fragments arranged by size:
860 363 109
BseYI C'CCAG_C
Cuts at: 0 77 292 1073 1332
Size: 77 215 781 259
Fragments arranged by size:
781 259 215 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 452 524 1228 1332
Size: 394 58 72 704 104
Fragments arranged by size:
704 394 104 72 58
BsiEI CG_ry'CG
Cuts at: 0 117 1332
Size: 117 1215
BsiHKAI G_wGCw'C
Cuts at: 0 1154 1332
Size: 1154 178
Bsp1286I G_dGCh'C
Cuts at: 0 166 305 371 548 621 812 1154 1332
Size: 166 139 66 177 73 191 342 178
Fragments arranged by size:
342 191 178 177 166 139 73 66
BspHI T'CATG_A
Cuts at: 0 371 1044 1332
Size: 371 673 288
Fragments arranged by size:
673 371 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 413 1332
Size: 413 919
BsrBI CCG'CTC
Cuts at: 0 99 360 1332
Size: 99 261 972
Fragments arranged by size:
972 261 99
BsrDI GCAATG_nn'
Cuts at: 0 1098 1332
Size: 1098 234
BsrFI r'CCGG_y
Cuts at: 0 149 1332
Size: 149 1183
BsrGI T'GTAC_A
Cuts at: 0 125 1332
Size: 125 1207
BstAPI GCAn_nnn'nTGC
Cuts at: 0 417 1332
Size: 417 915
BstYI r'GATC_y
Cuts at: 0 204 728 1332
Size: 204 524 604
Fragments arranged by size:
604 524 204
BtgI C'CryG_G
Cuts at: 0 29 200 1332
Size: 29 171 1132
Fragments arranged by size:
1132 171 29
DraIII CAC_nnn'GTG
Cuts at: 0 1194 1332
Size: 1194 138
EaeI y'GGCC_r
Cuts at: 0 17 603 1332
Size: 17 586 729
Fragments arranged by size:
729 586 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 543 631 1063 1332
Size: 266 277 88 432 269
Fragments arranged by size:
432 277 269 266 88
EcoO109I rG'GnC_Cy
Cuts at: 0 40 234 617 618 809 1332
Size: 40 194 383 1 191 523
Fragments arranged by size:
523 383 194 191 40 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1125 1332
Size: 1125 207
HaeII r_GCGC'y
Cuts at: 0 84 1332
Size: 84 1248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 18 50 1228 1260 1332
Size: 18 32 1178 32 72
Fragments arranged by size:
1178 72 32 32 18
MscI TGG'CCA
Cuts at: 0 605 1332
Size: 605 727
MslI CAynn'nnrTG
Cuts at: 0 697 928 1332
Size: 697 231 404
Fragments arranged by size:
697 404 231
MspA1I CmG'CkG
Cuts at: 0 31 1332
Size: 31 1301
NaeI GCC'GGC
Cuts at: 0 151 1332
Size: 151 1181
NgoMIV G'CCGG_C
Cuts at: 0 149 1332
Size: 149 1183
NsiI A_TGCA'T
Cuts at: 0 704 1332
Size: 704 628
NspI r_CATG'y
Cuts at: 0 645 702 1091 1332
Size: 645 57 389 241
Fragments arranged by size:
645 389 241 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 632 1194 1332
Size: 632 562 138
Fragments arranged by size:
632 562 138
PfoI T'CCnGG_A
Cuts at: 0 335 983 1332
Size: 335 648 349
Fragments arranged by size:
648 349 335
PpuMI rG'GwC_Cy
Cuts at: 0 40 1332
Size: 40 1292
PspOMI G'GGCC_C
Cuts at: 0 617 808 1332
Size: 617 191 524
Fragments arranged by size:
617 524 191
PstI C_TGCA'G
Cuts at: 0 533 1332
Size: 533 799
SacII CC_GC'GG
Cuts at: 0 32 1332
Size: 32 1300
SfcI C'TryA_G
Cuts at: 0 529 1332
Size: 529 803
SmaI CCC'GGG
Cuts at: 0 169 1332
Size: 169 1163
StyI C'CwwG_G
Cuts at: 0 719 1332
Size: 719 613
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 543 867 996 1087 1332
Size: 543 324 129 91 245
Fragments arranged by size:
543 324 245 129 91
TatI w'GTAC_w
Cuts at: 0 125 1332
Size: 125 1207
XmaI C'CCGG_G
Cuts at: 0 167 1332
Size: 167 1165
XmnI GAAnn'nnTTC
Cuts at: 0 952 1332
Size: 952 380
ZraI GAC'GTC
Cuts at: 0 464 1332
Size: 464 868
Enzymes that do cut:
AatII AcuI AfeI AleI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BclI BfrBI Bme1580I BmrI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstYI BtgI DraIII EaeI Eco57MI EcoO109I
FalI HaeII Hin4I MscI MslI MspA1I NaeI NgoMIV
NsiI NspI PflMI PfoI PpuMI PspOMI PstI SacII
SfcI SmaI StyI TaqII TatI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BglI BglII BlpI
BmgBI BmtI BplI BpuEI BsaAI BsaXI BsiWI BsmI
BsmBI BspEI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI