(Linear) (Six-base) MAPSORT of: KIEE4426.seq  Check: 7691  from: 1  to: 1329



KIEE4426.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     918    1329 
   Size:        918     411
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1137    1329 
   Size:       1137     192
 
AleI CACnn'nnGTG

Cuts at:      0     938    1123    1329 
   Size:        938     185     206

  Fragments arranged by size: 

                 938     206     185
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1099    1329 
   Size:       1099     230
 
ApaI G_GGCC'C

Cuts at:      0     819    1329 
   Size:        819     510
 
ApaLI G'TGCA_C

Cuts at:      0     931    1329 
   Size:        931     398
 
ApoI r'AATT_y

Cuts at:      0     462    1251    1329 
   Size:        462     789      78

  Fragments arranged by size: 

                 789     462      78
 
AseI AT'TA_AT

Cuts at:      0     910    1329 
   Size:        910     419
 
AvrII C'CTAG_G

Cuts at:      0     936    1329 
   Size:        936     393
 
BanI G'GyrC_C

Cuts at:      0     574    1329 
   Size:        574     755
 
BanII G_rGCy'C

Cuts at:      0     819    1329 
   Size:        819     510
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     103    1329 
   Size:        103    1226
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     347     757     785    1329 
   Size:        347     410      28     544

  Fragments arranged by size: 

                 544     410     347      28
 
BclI T'GATC_A

Cuts at:      0     338     960    1329 
   Size:        338     622     369

  Fragments arranged by size: 

                 622     369     338
 
BfrBI ATG'CAT

Cuts at:      0     246    1329 
   Size:        246    1083
 
BglI GCCn_nnn'nGGC

Cuts at:      0     583    1329 
   Size:        583     746
 
Bme1580I G_kGCm'C

Cuts at:      0     579     819     935    1329 
   Size:        579     240     116     394

  Fragments arranged by size: 

                 579     394     240     116
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     629     813    1204    1329 
   Size:        629     184     391     125

  Fragments arranged by size: 

                 629     391     184     125
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480    1329 
   Size:        480     849
 
BsaAI yAC'GTr

Cuts at:      0     234    1329 
   Size:        234    1095
 
BsaBI GATnn'nnATC

Cuts at:      0     358    1329 
   Size:        358     971
 
BsaWI w'CCGG_w

Cuts at:      0    1134    1329 
   Size:       1134     195
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     883    1329 
   Size:        883     446
 
BsiHKAI G_wGCw'C

Cuts at:      0     935    1161    1329 
   Size:        935     226     168

  Fragments arranged by size: 

                 935     226     168
 
BsmI GAATG_Cn'

Cuts at:      0     797    1329 
   Size:        797     532
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     535     710    1329 
   Size:        535     175     619

  Fragments arranged by size: 

                 619     535     175
 
Bsp1286I G_dGCh'C

Cuts at:      0     579     819     935    1161    1329 
   Size:        579     240     116     226     168

  Fragments arranged by size: 

                 579     240     226     168     116
 
BspEI T'CCGG_A

Cuts at:      0    1134    1329 
   Size:       1134     195
 
BspHI T'CATG_A

Cuts at:      0     521    1329 
   Size:        521     808
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     918    1329 
   Size:        918     411
 
BssSI C'ACGA_G

Cuts at:      0     580     863    1329 
   Size:        580     283     466

  Fragments arranged by size: 

                 580     466     283
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     887    1329 
   Size:        887     442
 
BtgI C'CryG_G

Cuts at:      0     567    1329 
   Size:        567     762
 
EaeI y'GGCC_r

Cuts at:      0     584    1329 
   Size:        584     745
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1137    1329 
   Size:       1137     192
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     279     311    1329 
   Size:        279      32    1018

  Fragments arranged by size: 

                1018     279      32
 
HindIII A'AGCT_T

Cuts at:      0     639     874    1329 
   Size:        639     235     455

  Fragments arranged by size: 

                 639     455     235
 
MfeI C'AATT_G

Cuts at:      0    1195    1329 
   Size:       1195     134
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     818    1329 
   Size:        818     511
 
MscI TGG'CCA

Cuts at:      0     586    1329 
   Size:        586     743
 
MslI CAynn'nnrTG

Cuts at:      0     251     572     683     938    1123    1329 
   Size:        251     321     111     255     185     206

  Fragments arranged by size: 

                 321     255     251     206     185     111
 
MspA1I CmG'CkG

Cuts at:      0    1270    1329 
   Size:       1270      59
 
NcoI C'CATG_G

Cuts at:      0     567    1329 
   Size:        567     762
 
NsiI A_TGCA'T

Cuts at:      0     248    1329 
   Size:        248    1081
 
PshAI GACnn'nnGTC

Cuts at:      0     547    1329 
   Size:        547     782
 
PsiI TTA'TAA

Cuts at:      0     440    1329 
   Size:        440     889
 
PspOMI G'GGCC_C

Cuts at:      0     815    1329 
   Size:        815     514
 
PvuII CAG'CTG

Cuts at:      0    1270    1329 
   Size:       1270      59
 
SfcI C'TryA_G

Cuts at:      0     114     417    1026    1329 
   Size:        114     303     609     303

  Fragments arranged by size: 

                 609     303     303     114
 
SmlI C'TyrA_G

Cuts at:      0     495    1329 
   Size:        495     834
 
StyI C'CwwG_G

Cuts at:      0     567     783     936    1188    1329 
   Size:        567     216     153     252     141

  Fragments arranged by size: 

                 567     252     216     153     141
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27    1329 
   Size:         27    1302
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     990    1329 
   Size:        990     339
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AvrII     BanI    BanII     BbsI    BciVI     BclI    BfrBI     BglI
 Bme1580I     BmrI    BpuEI    BsaAI    BsaBI    BsaWI    BseRI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BssSI    BstXI
     BtgI     EaeI  Eco57MI    Hin4I  HindIII     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NsiI    PshAI     PsiI   PspOMI    PvuII
     SfcI     SmlI     StyI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     AscI    AsiSI     AvaI     BaeI    BamHI
     BbeI    BbvCI     BcgI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI