Restriction map for
pFN21AE4425
(Linear) (Six-base) MAPSORT of: KIEE4425.seq Check: 5272 from: 1 to: 1320
KIEE4425.orf
With 182 enzymes: SgfI *
March 22, 2012 17:59 ..
Acc65I G'GTAC_C
Cuts at: 0 1064 1320
Size: 1064 256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 982 1110 1320
Size: 596 386 128 210
Fragments arranged by size:
596 386 210 128
AflIII A'CryG_T
Cuts at: 0 868 1320
Size: 868 452
AhdI GACnn_n'nnGTC
Cuts at: 0 783 1320
Size: 783 537
AlwNI CAG_nnn'CTG
Cuts at: 0 208 681 1015 1320
Size: 208 473 334 305
Fragments arranged by size:
473 334 305 208
ApaI G_GGCC'C
Cuts at: 0 795 1320
Size: 795 525
ApoI r'AATT_y
Cuts at: 0 99 145 939 1102 1320
Size: 99 46 794 163 218
Fragments arranged by size:
794 218 163 99 46
AvaI C'yCGr_G
Cuts at: 0 415 788 1320
Size: 415 373 532
Fragments arranged by size:
532 415 373
AvrII C'CTAG_G
Cuts at: 0 264 1320
Size: 264 1056
BamHI G'GATC_C
Cuts at: 0 313 1320
Size: 313 1007
BanI G'GyrC_C
Cuts at: 0 1064 1185 1320
Size: 1064 121 135
Fragments arranged by size:
1064 135 121
BanII G_rGCy'C
Cuts at: 0 795 1023 1163 1320
Size: 795 228 140 157
Fragments arranged by size:
795 228 157 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 838 872 1320
Size: 838 34 448
Fragments arranged by size:
838 448 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 427 1320
Size: 427 893
Bme1580I G_kGCm'C
Cuts at: 0 795 1320
Size: 795 525
BmgBI CAC'GTC
Cuts at: 0 869 1320
Size: 869 451
BmrI ACTGGGnnnn_n'
Cuts at: 0 1017 1320
Size: 1017 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1037 1320
Size: 944 93 283
Fragments arranged by size:
944 283 93
Bpu10I CC'TnA_GC
Cuts at: 0 827 1320
Size: 827 493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1054 1320
Size: 856 198 266
Fragments arranged by size:
856 266 198
BsaI GGTCTCn'nnnn_
Cuts at: 0 1030 1320
Size: 1030 290
BsaBI GATnn'nnATC
Cuts at: 0 254 1320
Size: 254 1066
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 889 1320
Size: 889 431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1320
Size: 257 1063
BsiHKAI G_wGCw'C
Cuts at: 0 17 390 881 1163 1320
Size: 17 373 491 282 157
Fragments arranged by size:
491 373 282 157 17
Bsp1286I G_dGCh'C
Cuts at: 0 17 390 795 881 1023 1163 1320
Size: 17 373 405 86 142 140 157
Fragments arranged by size:
405 373 157 142 140 86 17
BsrBI CCG'CTC
Cuts at: 0 428 1320
Size: 428 892
BsrDI GCAATG_nn'
Cuts at: 0 854 1320
Size: 854 466
BssSI C'ACGA_G
Cuts at: 0 400 1320
Size: 400 920
BstAPI GCAn_nnn'nTGC
Cuts at: 0 208 244 1320
Size: 208 36 1076
Fragments arranged by size:
1076 208 36
BstYI r'GATC_y
Cuts at: 0 313 772 1320
Size: 313 459 548
Fragments arranged by size:
548 459 313
BtgI C'CryG_G
Cuts at: 0 157 1073 1181 1259 1320
Size: 157 916 108 78 61
Fragments arranged by size:
916 157 108 78 61
BtsI GCAGTG_nn'
Cuts at: 0 53 573 1320
Size: 53 520 747
Fragments arranged by size:
747 520 53
EaeI y'GGCC_r
Cuts at: 0 1076 1320
Size: 1076 244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 780 1320
Size: 780 540
EcoICRI GAG'CTC
Cuts at: 0 1161 1320
Size: 1161 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 944 982 1037 1110 1320
Size: 596 348 38 55 73 210
Fragments arranged by size:
596 348 210 73 55 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1193 1320
Size: 1193 127
EcoO109I rG'GnC_Cy
Cuts at: 0 792 1320
Size: 792 528
EcoRI G'AATT_C
Cuts at: 0 145 1320
Size: 145 1175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 764 769 796 801 1320
Size: 764 5 27 5 519
Fragments arranged by size:
764 519 27 5 5
KpnI G_GTAC'C
Cuts at: 0 1068 1320
Size: 1068 252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 800 1320
Size: 341 459 520
Fragments arranged by size:
520 459 341
MslI CAynn'nnrTG
Cuts at: 0 189 1320
Size: 189 1131
MspA1I CmG'CkG
Cuts at: 0 655 1320
Size: 655 665
NcoI C'CATG_G
Cuts at: 0 157 1073 1320
Size: 157 916 247
Fragments arranged by size:
916 247 157
PflMI CCAn_nnn'nTGG
Cuts at: 0 163 1320
Size: 163 1157
PspOMI G'GGCC_C
Cuts at: 0 791 1320
Size: 791 529
PstI C_TGCA'G
Cuts at: 0 933 1320
Size: 933 387
PvuII CAG'CTG
Cuts at: 0 655 1320
Size: 655 665
SacI G_AGCT'C
Cuts at: 0 1163 1320
Size: 1163 157
SfcI C'TryA_G
Cuts at: 0 665 929 1190 1320
Size: 665 264 261 130
Fragments arranged by size:
665 264 261 130
SmaI CCC'GGG
Cuts at: 0 790 1320
Size: 790 530
SmlI C'TyrA_G
Cuts at: 0 835 1033 1320
Size: 835 198 287
Fragments arranged by size:
835 287 198
StyI C'CwwG_G
Cuts at: 0 157 264 342 562 1073 1231 1320
Size: 157 107 78 220 511 158 89
Fragments arranged by size:
511 220 158 157 107 89 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 7 1320
Size: 7 1313
XmaI C'CCGG_G
Cuts at: 0 788 1320
Size: 788 532
XmnI GAAnn'nnTTC
Cuts at: 0 116 956 1320
Size: 116 840 364
Fragments arranged by size:
840 364 116
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BcgI BciVI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BseRI BsgI
BsiHKAI Bsp1286I BsrBI BsrDI BssSI BstAPI BstYI BtgI
BtsI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
Hin4I KpnI MmeI MslI MspA1I NcoI PflMI PspOMI
PstI PvuII SacI SfcI SmaI SmlI StyI TaqII
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BbeI
BbsI BbvCI BclI BfrBI BglI BglII BlpI BmtI
BplI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI ZraI