Restriction map for
pFN21AE4424
(Linear) (Six-base) MAPSORT of: KIEE4424.seq Check: 6716 from: 1 to: 1317
KIEE4424.orf
With 182 enzymes: SgfI *
March 22, 2012 17:59 ..
AccI GT'mk_AC
Cuts at: 0 530 1317
Size: 530 787
AfeI AGC'GCT
Cuts at: 0 883 1317
Size: 883 434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 1317
Size: 154 1163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 451 483 1317
Size: 451 32 834
Fragments arranged by size:
834 451 32
AlwNI CAG_nnn'CTG
Cuts at: 0 591 1317
Size: 591 726
ApaI G_GGCC'C
Cuts at: 0 683 1182 1317
Size: 683 499 135
Fragments arranged by size:
683 499 135
AvaI C'yCGr_G
Cuts at: 0 710 1032 1082 1317
Size: 710 322 50 235
Fragments arranged by size:
710 322 235 50
BamHI G'GATC_C
Cuts at: 0 647 1317
Size: 647 670
BanI G'GyrC_C
Cuts at: 0 322 790 874 1231 1317
Size: 322 468 84 357 86
Fragments arranged by size:
468 357 322 86 84
BanII G_rGCy'C
Cuts at: 0 197 287 683 1182 1244 1317
Size: 197 90 396 499 62 73
Fragments arranged by size:
499 396 197 90 73 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 703 1317
Size: 703 614
BclI T'GATC_A
Cuts at: 0 368 1317
Size: 368 949
Bme1580I G_kGCm'C
Cuts at: 0 683 1182 1317
Size: 683 499 135
Fragments arranged by size:
683 499 135
BmgBI CAC'GTC
Cuts at: 0 1134 1317
Size: 1134 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 1176 1317
Size: 1176 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 522 1317
Size: 522 795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 952 1294 1317
Size: 502 450 342 23
Fragments arranged by size:
502 450 342 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1317
Size: 85 1232
BsaI GGTCTCn'nnnn_
Cuts at: 0 437 1317
Size: 437 880
BsaBI GATnn'nnATC
Cuts at: 0 157 296 1317
Size: 157 139 1021
Fragments arranged by size:
1021 157 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 325 355 434 451 464 481
Size: 253 30 42 30 79 17 13 17
Cuts at: 481 1317
Size: 836
Fragments arranged by size:
836 253 79 42 30 30 17 17
13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 130 328 1015 1090 1159 1317
Size: 106 24 198 687 75 69 158
Fragments arranged by size:
687 198 158 106 75 69 24
BseYI C'CCAG_C
Cuts at: 0 761 901 1317
Size: 761 140 416
Fragments arranged by size:
761 416 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 937 1143 1300 1317
Size: 790 147 206 157 17
Fragments arranged by size:
790 206 157 147 17
BsiEI CG_ry'CG
Cuts at: 0 56 1317
Size: 56 1261
BsiHKAI G_wGCw'C
Cuts at: 0 101 197 287 1244 1317
Size: 101 96 90 957 73
Fragments arranged by size:
957 101 96 90 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 478 1317
Size: 478 839
Bsp1286I G_dGCh'C
Cuts at: 0 101 197 287 683 1182 1244 1317
Size: 101 96 90 396 499 62 73
Fragments arranged by size:
499 396 101 96 90 73 62
BspHI T'CATG_A
Cuts at: 0 161 1317
Size: 161 1156
BsrBI CCG'CTC
Cuts at: 0 1031 1317
Size: 1031 286
BssSI C'ACGA_G
Cuts at: 0 1249 1317
Size: 1249 68
BstXI CCAn_nnnn'nTGG
Cuts at: 0 952 1317
Size: 952 365
BstYI r'GATC_y
Cuts at: 0 581 647 1100 1317
Size: 581 66 453 217
Fragments arranged by size:
581 453 217 66
BtgI C'CryG_G
Cuts at: 0 110 521 542 1235 1317
Size: 110 411 21 693 82
Fragments arranged by size:
693 411 110 82 21
BtsI GCAGTG_nn'
Cuts at: 0 1219 1317
Size: 1219 98
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 983 1018 1317
Size: 983 35 299
Fragments arranged by size:
983 299 35
EaeI y'GGCC_r
Cuts at: 0 821 1317
Size: 821 496
EarI CTCTTCn'nnn_
Cuts at: 0 160 1317
Size: 160 1157
EcoICRI GAG'CTC
Cuts at: 0 195 285 1242 1317
Size: 195 90 957 75
Fragments arranged by size:
957 195 90 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 952 1294 1317
Size: 502 450 342 23
Fragments arranged by size:
502 450 342 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 659 823 1317
Size: 659 164 494
Fragments arranged by size:
659 494 164
EcoO109I rG'GnC_Cy
Cuts at: 0 680 1178 1317
Size: 680 498 139
Fragments arranged by size:
680 498 139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 756 1317
Size: 756 561
FspI TGC'GCA
Cuts at: 0 1225 1317
Size: 1225 92
HaeII r_GCGC'y
Cuts at: 0 838 885 1317
Size: 838 47 432
Fragments arranged by size:
838 432 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 639 671 1317
Size: 325 32 282 32 646
Fragments arranged by size:
646 325 282 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 675 842 1317
Size: 296 379 167 475
Fragments arranged by size:
475 379 296 167
MscI TGG'CCA
Cuts at: 0 823 1317
Size: 823 494
MspA1I CmG'CkG
Cuts at: 0 765 948 1157 1317
Size: 765 183 209 160
Fragments arranged by size:
765 209 183 160
NcoI C'CATG_G
Cuts at: 0 521 1317
Size: 521 796
PasI CC'CwG_GG
Cuts at: 0 685 1317
Size: 685 632
PflMI CCAn_nnn'nTGG
Cuts at: 0 591 1317
Size: 591 726
PfoI T'CCnGG_A
Cuts at: 0 287 479 584 803 1010 1271 1317
Size: 287 192 105 219 207 261 46
Fragments arranged by size:
287 261 219 207 192 105 46
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 1317
Size: 451 32 834
Fragments arranged by size:
834 451 32
PspOMI G'GGCC_C
Cuts at: 0 679 1178 1317
Size: 679 499 139
Fragments arranged by size:
679 499 139
PvuII CAG'CTG
Cuts at: 0 765 948 1317
Size: 765 183 369
Fragments arranged by size:
765 369 183
SacI G_AGCT'C
Cuts at: 0 197 287 1244 1317
Size: 197 90 957 73
Fragments arranged by size:
957 197 90 73
SexAI A'CCwGG_T
Cuts at: 0 446 1317
Size: 446 871
SmaI CCC'GGG
Cuts at: 0 712 1084 1317
Size: 712 372 233
Fragments arranged by size:
712 372 233
SmlI C'TyrA_G
Cuts at: 0 100 1317
Size: 100 1217
SrfI GCCC'GGGC
Cuts at: 0 712 1084 1317
Size: 712 372 233
Fragments arranged by size:
712 372 233
SspI AAT'ATT
Cuts at: 0 18 330 1317
Size: 18 312 987
Fragments arranged by size:
987 312 18
StyI C'CwwG_G
Cuts at: 0 521 824 1317
Size: 521 303 493
Fragments arranged by size:
521 493 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 442 985 1261 1317
Size: 202 240 543 276 56
Fragments arranged by size:
543 276 240 202 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 434 466 1317
Size: 434 32 851
Fragments arranged by size:
851 434 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 316 333 999 1317
Size: 316 17 666 318
Fragments arranged by size:
666 318 316 17
XmaI C'CCGG_G
Cuts at: 0 710 1082 1317
Size: 710 372 235
Fragments arranged by size:
710 372 235
Enzymes that do cut:
AccI AfeI AlfI AloI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BclI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BssSI BstXI
BstYI BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I MmeI MscI
MspA1I NcoI PasI PflMI PfoI PpiI PspOMI PvuII
SacI SexAI SmaI SmlI SrfI SspI StyI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI BmtI Bpu10I BsaAI BsaHI BsaWI BsiWI BsmI
BspEI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI StuI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI