Restriction map for
pFN21AE4421
(Linear) (Six-base) MAPSORT of: KIEE4421.seq Check: 8204 from: 1 to: 1311
KIEE4421.orf
With 182 enzymes: SgfI *
March 22, 2012 17:58 ..
AccI GT'mk_AC
Cuts at: 0 670 1311
Size: 670 641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1311
Size: 148 1163
AhdI GACnn_n'nnGTC
Cuts at: 0 84 1311
Size: 84 1227
AleI CACnn'nnGTG
Cuts at: 0 175 406 686 1311
Size: 175 231 280 625
Fragments arranged by size:
625 280 231 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 554 586 1311
Size: 554 32 725
Fragments arranged by size:
725 554 32
AlwNI CAG_nnn'CTG
Cuts at: 0 436 1311
Size: 436 875
ApoI r'AATT_y
Cuts at: 0 781 818 1311
Size: 781 37 493
Fragments arranged by size:
781 493 37
AvaI C'yCGr_G
Cuts at: 0 41 1311
Size: 41 1270
BamHI G'GATC_C
Cuts at: 0 661 1311
Size: 661 650
BanII G_rGCy'C
Cuts at: 0 70 1311
Size: 70 1241
BbsI GAAGACnn'nnnn_
Cuts at: 0 169 457 1311
Size: 169 288 854
Fragments arranged by size:
854 288 169
BbvCI CC'TCA_GC
Cuts at: 0 90 1220 1311
Size: 90 1130 91
Fragments arranged by size:
1130 91 90
BciVI GTATCCnnnnn_n'
Cuts at: 0 128 1311
Size: 128 1183
BlpI GC'TnA_GC
Cuts at: 0 725 908 1311
Size: 725 183 403
Fragments arranged by size:
725 403 183
BmgBI CAC'GTC
Cuts at: 0 1161 1311
Size: 1161 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 347 380 667 1311
Size: 347 33 287 644
Fragments arranged by size:
644 347 287 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1311
Size: 946 365
Bpu10I CC'TnA_GC
Cuts at: 0 90 830 1220 1244 1311
Size: 90 740 390 24 67
Fragments arranged by size:
740 390 90 67 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 1276 1311
Size: 1276 35
BsaAI yAC'GTr
Cuts at: 0 74 925 971 1311
Size: 74 851 46 340
Fragments arranged by size:
851 340 74 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 554 584 1311
Size: 554 30 727
Fragments arranged by size:
727 554 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 1099 1311
Size: 286 813 212
Fragments arranged by size:
813 286 212
BseYI C'CCAG_C
Cuts at: 0 1286 1311
Size: 1286 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1277 1311
Size: 1277 34
BsiHKAI G_wGCw'C
Cuts at: 0 70 195 914 1311
Size: 70 125 719 397
Fragments arranged by size:
719 397 125 70
BsmI GAATG_Cn'
Cuts at: 0 1245 1311
Size: 1245 66
Bsp1286I G_dGCh'C
Cuts at: 0 70 195 914 1311
Size: 70 125 719 397
Fragments arranged by size:
719 397 125 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 1311
Size: 62 1249
BsrDI GCAATG_nn'
Cuts at: 0 1080 1311
Size: 1080 231
BssSI C'ACGA_G
Cuts at: 0 511 1311
Size: 511 800
BstAPI GCAn_nnn'nTGC
Cuts at: 0 10 349 1311
Size: 10 339 962
Fragments arranged by size:
962 339 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 1035 1311
Size: 462 573 276
Fragments arranged by size:
573 462 276
BstYI r'GATC_y
Cuts at: 0 661 1311
Size: 661 650
BtgI C'CryG_G
Cuts at: 0 176 1311
Size: 176 1135
BtsI GCAGTG_nn'
Cuts at: 0 437 447 525 1311
Size: 437 10 78 786
Fragments arranged by size:
786 437 78 10
DraI TTT'AAA
Cuts at: 0 1089 1311
Size: 1089 222
EaeI y'GGCC_r
Cuts at: 0 179 389 1311
Size: 179 210 922
Fragments arranged by size:
922 210 179
EcoICRI GAG'CTC
Cuts at: 0 68 1311
Size: 68 1243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 946 1311
Size: 148 798 365
Fragments arranged by size:
798 365 148
EcoO109I rG'GnC_Cy
Cuts at: 0 1207 1311
Size: 1207 104
HaeII r_GCGC'y
Cuts at: 0 332 1311
Size: 332 979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 177 209 735 767 1045 1077
Size: 70 32 75 32 526 32 278 32
Cuts at: 1077 1155 1187 1218 1250 1311
Size: 78 32 31 32 61
Fragments arranged by size:
526 278 78 75 70 61 32 32
32 32 32 32 31
HincII GTy'rAC
Cuts at: 0 51 1179 1311
Size: 51 1128 132
Fragments arranged by size:
1128 132 51
HindIII A'AGCT_T
Cuts at: 0 676 1311
Size: 676 635
HpaI GTT'AAC
Cuts at: 0 1179 1311
Size: 1179 132
MfeI C'AATT_G
Cuts at: 0 935 1311
Size: 935 376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 1013 1311
Size: 478 535 298
Fragments arranged by size:
535 478 298
MscI TGG'CCA
Cuts at: 0 181 391 1311
Size: 181 210 920
Fragments arranged by size:
920 210 181
MslI CAynn'nnrTG
Cuts at: 0 175 406 686 1039 1311
Size: 175 231 280 353 272
Fragments arranged by size:
353 280 272 231 175
MspA1I CmG'CkG
Cuts at: 0 1131 1224 1311
Size: 1131 93 87
Fragments arranged by size:
1131 93 87
PmlI CAC'GTG
Cuts at: 0 971 1311
Size: 971 340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 554 586 1311
Size: 554 32 725
Fragments arranged by size:
725 554 32
PpuMI rG'GwC_Cy
Cuts at: 0 1207 1311
Size: 1207 104
PstI C_TGCA'G
Cuts at: 0 1136 1311
Size: 1136 175
PvuII CAG'CTG
Cuts at: 0 1131 1224 1311
Size: 1131 93 87
Fragments arranged by size:
1131 93 87
SacI G_AGCT'C
Cuts at: 0 70 1311
Size: 70 1241
SfcI C'TryA_G
Cuts at: 0 1132 1311
Size: 1132 179
StyI C'CwwG_G
Cuts at: 0 59 377 996 1251 1311
Size: 59 318 619 255 60
Fragments arranged by size:
619 318 255 60 59
TatI w'GTAC_w
Cuts at: 0 103 1311
Size: 103 1208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 998 1030 1311
Size: 998 32 281
Fragments arranged by size:
998 281 32
Tth111I GACn'n_nGTC
Cuts at: 0 456 1230 1311
Size: 456 774 81
Fragments arranged by size:
774 456 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 384 1311
Size: 174 210 927
Fragments arranged by size:
927 210 174
Enzymes that do cut:
AccI AcuI AhdI AleI AloI AlwNI ApoI AvaI
BamHI BanII BbsI BbvCI BciVI BlpI BmgBI BmrI
BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI
BstYI BtgI BtsI DraI EaeI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI
MslI MspA1I PmlI PpiI PpuMI PstI PvuII SacI
SfcI StyI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BcgI BclI BfrBI BglI BglII Bme1580I
BmtI BplI BpuEI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI KasI KpnI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII XbaI XhoI XmaI XmnI ZraI