Restriction map for
pFN21AE4419
(Linear) (Six-base) MAPSORT of: KIEE4419.seq Check: 7041 from: 1 to: 1296
KIEE4419.orf
With 182 enzymes: SgfI *
March 22, 2012 17:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 960 1296
Size: 960 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1205 1296
Size: 154 1051 91
Fragments arranged by size:
1051 154 91
AgeI A'CCGG_T
Cuts at: 0 977 1296
Size: 977 319
AleI CACnn'nnGTG
Cuts at: 0 622 1296
Size: 622 674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1027 1296
Size: 1027 269
AlwNI CAG_nnn'CTG
Cuts at: 0 351 438 693 1183 1248 1296
Size: 351 87 255 490 65 48
Fragments arranged by size:
490 351 255 87 65 48
ApaI G_GGCC'C
Cuts at: 0 371 1296
Size: 371 925
BamHI G'GATC_C
Cuts at: 0 512 1296
Size: 512 784
BanI G'GyrC_C
Cuts at: 0 139 522 998 1296
Size: 139 383 476 298
Fragments arranged by size:
476 383 298 139
BanII G_rGCy'C
Cuts at: 0 371 711 1296
Size: 371 340 585
Fragments arranged by size:
585 371 340
BbeI G_GCGC'C
Cuts at: 0 143 1002 1296
Size: 143 859 294
Fragments arranged by size:
859 294 143
BbvCI CC'TCA_GC
Cuts at: 0 215 1296
Size: 215 1081
Bme1580I G_kGCm'C
Cuts at: 0 371 687 1296
Size: 371 316 609
Fragments arranged by size:
609 371 316
BmgBI CAC'GTC
Cuts at: 0 909 1296
Size: 909 387
BmrI ACTGGGnnnn_n'
Cuts at: 0 485 1236 1296
Size: 485 751 60
Fragments arranged by size:
751 485 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 132 1296
Size: 132 1164
Bpu10I CC'TnA_GC
Cuts at: 0 215 1296
Size: 215 1081
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1296
Size: 502 794
BsaI GGTCTCn'nnnn_
Cuts at: 0 187 460 1296
Size: 187 273 836
Fragments arranged by size:
836 273 187
BsaHI Gr'CG_yC
Cuts at: 0 140 999 1296
Size: 140 859 297
Fragments arranged by size:
859 297 140
BsaWI w'CCGG_w
Cuts at: 0 291 977 1296
Size: 291 686 319
Fragments arranged by size:
686 319 291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 229 259 316 346 698 728
Size: 106 30 93 30 57 30 352 30
Cuts at: 728 1296
Size: 568
Fragments arranged by size:
568 352 106 93 57 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 478 1296
Size: 337 141 818
Fragments arranged by size:
818 337 141
BseYI C'CCAG_C
Cuts at: 0 1060 1296
Size: 1060 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 241 1296
Size: 145 96 1055
Fragments arranged by size:
1055 145 96
BsiEI CG_ry'CG
Cuts at: 0 182 1296
Size: 182 1114
BsmI GAATG_Cn'
Cuts at: 0 287 1296
Size: 287 1009
BsmBI CGTCTCn'nnnn_
Cuts at: 0 169 1296
Size: 169 1127
Bsp1286I G_dGCh'C
Cuts at: 0 371 687 711 1296
Size: 371 316 24 585
Fragments arranged by size:
585 371 316 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 960 1296
Size: 960 336
BsrBI CCG'CTC
Cuts at: 0 111 1296
Size: 111 1185
BsrDI GCAATG_nn'
Cuts at: 0 68 272 1296
Size: 68 204 1024
Fragments arranged by size:
1024 204 68
BsrFI r'CCGG_y
Cuts at: 0 634 851 977 1296
Size: 634 217 126 319
Fragments arranged by size:
634 319 217 126
BstAPI GCAn_nnn'nTGC
Cuts at: 0 438 1296
Size: 438 858
BstEII G'GTnAC_C
Cuts at: 0 774 1296
Size: 774 522
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1167 1296
Size: 1167 129
BstYI r'GATC_y
Cuts at: 0 512 1296
Size: 512 784
BtgI C'CryG_G
Cuts at: 0 170 758 782 944 1049 1296
Size: 170 588 24 162 105 247
Fragments arranged by size:
588 247 170 162 105 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 132 1296
Size: 132 1164
BtsI GCAGTG_nn'
Cuts at: 0 229 745 1184 1296
Size: 229 516 439 112
Fragments arranged by size:
516 439 229 112
ClaI AT'CG_AT
Cuts at: 0 917 1296
Size: 917 379
EaeI y'GGCC_r
Cuts at: 0 179 503 849 1016 1296
Size: 179 324 346 167 280
Fragments arranged by size:
346 324 280 179 167
EagI C'GGCC_G
Cuts at: 0 179 1296
Size: 179 1117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1205 1296
Size: 154 1051 91
Fragments arranged by size:
1051 154 91
EcoO109I rG'GnC_Cy
Cuts at: 0 60 368 690 1296
Size: 60 308 322 606
Fragments arranged by size:
606 322 308 60
HaeII r_GCGC'y
Cuts at: 0 143 1002 1296
Size: 143 859 294
Fragments arranged by size:
859 294 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 698 730 1296
Size: 698 32 566
Fragments arranged by size:
698 566 32
HincII GTy'rAC
Cuts at: 0 1035 1217 1296
Size: 1035 182 79
Fragments arranged by size:
1035 182 79
KasI G'GCGC_C
Cuts at: 0 139 998 1296
Size: 139 859 298
Fragments arranged by size:
859 298 139
MscI TGG'CCA
Cuts at: 0 505 1296
Size: 505 791
MslI CAynn'nnrTG
Cuts at: 0 169 292 622 1296
Size: 169 123 330 674
Fragments arranged by size:
674 330 169 123
MspA1I CmG'CkG
Cuts at: 0 233 1296
Size: 233 1063
NaeI GCC'GGC
Cuts at: 0 636 853 1296
Size: 636 217 443
Fragments arranged by size:
636 443 217
NarI GG'CG_CC
Cuts at: 0 140 999 1296
Size: 140 859 297
Fragments arranged by size:
859 297 140
NcoI C'CATG_G
Cuts at: 0 758 782 1049 1296
Size: 758 24 267 247
Fragments arranged by size:
758 267 247 24
NgoMIV G'CCGG_C
Cuts at: 0 634 851 1296
Size: 634 217 445
Fragments arranged by size:
634 445 217
NspI r_CATG'y
Cuts at: 0 1257 1296
Size: 1257 39
PasI CC'CwG_GG
Cuts at: 0 207 1296
Size: 207 1089
PflMI CCAn_nnn'nTGG
Cuts at: 0 1098 1248 1296
Size: 1098 150 48
Fragments arranged by size:
1098 150 48
PshAI GACnn'nnGTC
Cuts at: 0 181 199 478 1296
Size: 181 18 279 818
Fragments arranged by size:
818 279 181 18
PspOMI G'GGCC_C
Cuts at: 0 367 1296
Size: 367 929
PstI C_TGCA'G
Cuts at: 0 752 1296
Size: 752 544
PvuII CAG'CTG
Cuts at: 0 233 1296
Size: 233 1063
SexAI A'CCwGG_T
Cuts at: 0 1091 1296
Size: 1091 205
SfcI C'TryA_G
Cuts at: 0 748 1296
Size: 748 548
SfoI GGC'GCC
Cuts at: 0 141 1000 1296
Size: 141 859 296
Fragments arranged by size:
859 296 141
SmlI C'TyrA_G
Cuts at: 0 517 1296
Size: 517 779
StuI AGG'CCT
Cuts at: 0 793 1296
Size: 793 503
StyI C'CwwG_G
Cuts at: 0 278 758 782 846 1049 1296
Size: 278 480 24 64 203 247
Fragments arranged by size:
480 278 247 203 64 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 265 1296
Size: 199 66 1031
Fragments arranged by size:
1031 199 66
TatI w'GTAC_w
Cuts at: 0 353 1296
Size: 353 943
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 1296
Size: 316 32 948
Fragments arranged by size:
948 316 32
Tth111I GACn'n_nGTC
Cuts at: 0 547 1296
Size: 547 749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1095 1296
Size: 1095 201
Enzymes that do cut:
AarI AcuI AgeI AleI AlfI AlwNI ApaI BamHI
BanI BanII BbeI BbvCI Bme1580I BmgBI BmrI BplI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI EaeI EagI Eco57MI EcoO109I HaeII Hin4I HincII
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PshAI PspOMI PstI PvuII SexAI
SfcI SfoI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmtI BpmI BsaAI BsaBI BsiHKAI BsiWI BspEI
BspHI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI
XmnI ZraI