Restriction map for
pFN21AE4418
(Linear) (Six-base) MAPSORT of: KIEE4418.seq Check: 4659 from: 1 to: 1293
KIEE4418.orf
With 182 enzymes: SgfI *
March 22, 2012 17:57 ..
Acc65I G'GTAC_C
Cuts at: 0 1271 1293
Size: 1271 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1239 1293
Size: 941 298 54
Fragments arranged by size:
941 298 54
AhdI GACnn_n'nnGTC
Cuts at: 0 1127 1293
Size: 1127 166
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 696 728 1293
Size: 696 32 565
Fragments arranged by size:
696 565 32
AlwNI CAG_nnn'CTG
Cuts at: 0 150 220 561 1293
Size: 150 70 341 732
Fragments arranged by size:
732 341 150 70
ApaLI G'TGCA_C
Cuts at: 0 131 1060 1210 1293
Size: 131 929 150 83
Fragments arranged by size:
929 150 131 83
ApoI r'AATT_y
Cuts at: 0 250 948 1293
Size: 250 698 345
Fragments arranged by size:
698 345 250
AvaI C'yCGr_G
Cuts at: 0 1146 1228 1293
Size: 1146 82 65
Fragments arranged by size:
1146 82 65
BanI G'GyrC_C
Cuts at: 0 1271 1293
Size: 1271 22
BclI T'GATC_A
Cuts at: 0 50 1293
Size: 50 1243
BfrBI ATG'CAT
Cuts at: 0 887 1293
Size: 887 406
Bme1580I G_kGCm'C
Cuts at: 0 135 1064 1187 1214 1293
Size: 135 929 123 27 79
Fragments arranged by size:
929 135 123 79 27
BmgBI CAC'GTC
Cuts at: 0 1241 1293
Size: 1241 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 383 484 1293
Size: 383 101 809
Fragments arranged by size:
809 383 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1293
Size: 704 589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 951 1293
Size: 755 196 342
Fragments arranged by size:
755 342 196
BsaI GGTCTCn'nnnn_
Cuts at: 0 454 1293
Size: 454 839
BsaAI yAC'GTr
Cuts at: 0 530 831 1293
Size: 530 301 462
Fragments arranged by size:
530 462 301
BsaHI Gr'CG_yC
Cuts at: 0 1053 1293
Size: 1053 240
BsaWI w'CCGG_w
Cuts at: 0 1064 1274 1293
Size: 1064 210 19
Fragments arranged by size:
1064 210 19
BseYI C'CCAG_C
Cuts at: 0 1139 1293
Size: 1139 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1263 1293
Size: 1263 30
BsiHKAI G_wGCw'C
Cuts at: 0 135 1064 1214 1293
Size: 135 929 150 79
Fragments arranged by size:
929 150 135 79
Bsp1286I G_dGCh'C
Cuts at: 0 135 1064 1187 1214 1293
Size: 135 929 123 27 79
Fragments arranged by size:
929 135 123 79 27
BspHI T'CATG_A
Cuts at: 0 449 1235 1293
Size: 449 786 58
Fragments arranged by size:
786 449 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1178 1293
Size: 1178 115
BsrBI CCG'CTC
Cuts at: 0 1161 1293
Size: 1161 132
BsrGI T'GTAC_A
Cuts at: 0 2 1293
Size: 2 1291
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 1293
Size: 150 1143
BstBI TT'CG_AA
Cuts at: 0 498 1293
Size: 498 795
BstYI r'GATC_y
Cuts at: 0 557 1293
Size: 557 736
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 72 1262 1293
Size: 72 1190 31
Fragments arranged by size:
1190 72 31
ClaI AT'CG_AT
Cuts at: 0 882 1293
Size: 882 411
DraIII CAC_nnn'GTG
Cuts at: 0 492 1293
Size: 492 801
EaeI y'GGCC_r
Cuts at: 0 422 1293
Size: 422 871
EarI CTCTTCn'nnn_
Cuts at: 0 725 1293
Size: 725 568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 941 1239 1293
Size: 704 237 298 54
Fragments arranged by size:
704 298 237 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 233 1293
Size: 233 1060
EcoRV GAT'ATC
Cuts at: 0 735 1293
Size: 735 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 486 518 1113 1145 1293
Size: 486 32 595 32 148
Fragments arranged by size:
595 486 148 32 32
KpnI G_GTAC'C
Cuts at: 0 1275 1293
Size: 1275 18
MfeI C'AATT_G
Cuts at: 0 203 1293
Size: 203 1090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 1293
Size: 681 612
MscI TGG'CCA
Cuts at: 0 424 1293
Size: 424 869
MslI CAynn'nnrTG
Cuts at: 0 518 785 914 1293
Size: 518 267 129 379
Fragments arranged by size:
518 379 267 129
MspA1I CmG'CkG
Cuts at: 0 63 1293
Size: 63 1230
NsiI A_TGCA'T
Cuts at: 0 889 1293
Size: 889 404
NspI r_CATG'y
Cuts at: 0 305 1293
Size: 305 988
PmlI CAC'GTG
Cuts at: 0 530 1293
Size: 530 763
PspXI vC'TCGA_Gb
Cuts at: 0 1146 1293
Size: 1146 147
PvuII CAG'CTG
Cuts at: 0 63 1293
Size: 63 1230
SfcI C'TryA_G
Cuts at: 0 345 983 1293
Size: 345 638 310
Fragments arranged by size:
638 345 310
SmaI CCC'GGG
Cuts at: 0 1230 1293
Size: 1230 63
SmlI C'TyrA_G
Cuts at: 0 770 966 1146 1293
Size: 770 196 180 147
Fragments arranged by size:
770 196 180 147
SnaBI TAC'GTA
Cuts at: 0 831 1293
Size: 831 462
SspI AAT'ATT
Cuts at: 0 1194 1293
Size: 1194 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 503 560 1293
Size: 503 57 733
Fragments arranged by size:
733 503 57
TatI w'GTAC_w
Cuts at: 0 2 1293
Size: 2 1291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1043 1075 1293
Size: 1043 32 218
Fragments arranged by size:
1043 218 32
Tth111I GACn'n_nGTC
Cuts at: 0 273 1293
Size: 273 1020
XhoI C'TCGA_G
Cuts at: 0 1146 1293
Size: 1146 147
XmaI C'CCGG_G
Cuts at: 0 1228 1293
Size: 1228 65
Enzymes that do cut:
Acc65I AcuI AhdI AloI AlwNI ApaLI ApoI AvaI
BanI BclI BfrBI Bme1580I BmgBI BmrI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrGI BstAPI BstBI BstYI BtgZI
ClaI DraIII EaeI EarI Eco57MI EcoNI EcoRV Hin4I
KpnI MfeI MmeI MscI MslI MspA1I NsiI NspI
PmlI PspXI PvuII SfcI SmaI SmlI SnaBI SspI
TaqII TatI TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbsI BbvCI BcgI BciVI BglI
BglII BlpI BmtI BplI Bpu10I BsaBI BsaXI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BssHII
BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtsI CspCI
DraI DrdI EagI EciI EcoICRI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SpeI SphI SrfI StuI
StyI SwaI XbaI XcmI XmnI ZraI