Restriction map for
pFN21AE4416
(Linear) (Six-base) MAPSORT of: KIEE4416.seq Check: 6172 from: 1 to: 1287
KIEE4416.orf
With 182 enzymes: SgfI *
March 22, 2012 17:57 ..
Acc65I G'GTAC_C
Cuts at: 0 983 1287
Size: 983 304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 406 916 1228 1287
Size: 301 105 510 312 59
Fragments arranged by size:
510 312 301 105 59
AfeI AGC'GCT
Cuts at: 0 145 334 1287
Size: 145 189 953
Fragments arranged by size:
953 189 145
AflIII A'CryG_T
Cuts at: 0 557 1287
Size: 557 730
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1255 1287
Size: 1255 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1234 1287
Size: 1234 53
ApoI r'AATT_y
Cuts at: 0 502 534 681 1287
Size: 502 32 147 606
Fragments arranged by size:
606 502 147 32
BanI G'GyrC_C
Cuts at: 0 983 1287
Size: 983 304
BanII G_rGCy'C
Cuts at: 0 224 1287
Size: 224 1063
BbvCI CC'TCA_GC
Cuts at: 0 7 329 823 1287
Size: 7 322 494 464
Fragments arranged by size:
494 464 322 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 445 479 647 681 1287
Size: 445 34 168 34 606
Fragments arranged by size:
606 445 168 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 442 1287
Size: 442 845
BglI GCCn_nnn'nGGC
Cuts at: 0 859 1287
Size: 859 428
BglII A'GATC_T
Cuts at: 0 492 1287
Size: 492 795
BlpI GC'TnA_GC
Cuts at: 0 1038 1287
Size: 1038 249
BmrI ACTGGGnnnn_n'
Cuts at: 0 147 492 1287
Size: 147 345 795
Fragments arranged by size:
795 345 147
BmtI G_CTAG'C
Cuts at: 0 361 1287
Size: 361 926
Bpu10I CC'TnA_GC
Cuts at: 0 7 329 823 1287
Size: 7 322 494 464
Fragments arranged by size:
494 464 322 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 1287
Size: 899 388
BsaI GGTCTCn'nnnn_
Cuts at: 0 234 1287
Size: 234 1053
BsaHI Gr'CG_yC
Cuts at: 0 115 1287
Size: 115 1172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1145 1175 1287
Size: 1145 30 112
Fragments arranged by size:
1145 112 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1150 1287
Size: 1150 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1287
Size: 118 1169
BsiHKAI G_wGCw'C
Cuts at: 0 93 224 1287
Size: 93 131 1063
Fragments arranged by size:
1063 131 93
Bsp1286I G_dGCh'C
Cuts at: 0 93 224 1287
Size: 93 131 1063
Fragments arranged by size:
1063 131 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 241 430 1287
Size: 241 189 857
Fragments arranged by size:
857 241 189
BsrFI r'CCGG_y
Cuts at: 0 1064 1287
Size: 1064 223
BssHII G'CGCG_C
Cuts at: 0 35 1287
Size: 35 1252
BstXI CCAn_nnnn'nTGG
Cuts at: 0 782 1287
Size: 782 505
BstYI r'GATC_y
Cuts at: 0 492 1287
Size: 492 795
BtgI C'CryG_G
Cuts at: 0 122 1287
Size: 122 1165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1083 1287
Size: 1083 204
EarI CTCTTCn'nnn_
Cuts at: 0 167 208 1287
Size: 167 41 1079
Fragments arranged by size:
1079 167 41
EcoICRI GAG'CTC
Cuts at: 0 222 1287
Size: 222 1065
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 406 916 1228 1287
Size: 301 105 510 312 59
Fragments arranged by size:
510 312 301 105 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1031 1287
Size: 1031 256
EcoO109I rG'GnC_Cy
Cuts at: 0 963 1287
Size: 963 324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 331 741 946 1287
Size: 331 410 205 341
Fragments arranged by size:
410 341 331 205
FspI TGC'GCA
Cuts at: 0 426 1287
Size: 426 861
HaeII r_GCGC'y
Cuts at: 0 147 336 1287
Size: 147 189 951
Fragments arranged by size:
951 189 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 721 753 1287
Size: 721 32 534
Fragments arranged by size:
721 534 32
KpnI G_GTAC'C
Cuts at: 0 987 1287
Size: 987 300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 390 681 763 924 1287
Size: 262 128 291 82 161 363
Fragments arranged by size:
363 291 262 161 128 82
MslI CAynn'nnrTG
Cuts at: 0 211 1287
Size: 211 1076
MspA1I CmG'CkG
Cuts at: 0 6 827 1287
Size: 6 821 460
Fragments arranged by size:
821 460 6
NcoI C'CATG_G
Cuts at: 0 122 1287
Size: 122 1165
NheI G'CTAG_C
Cuts at: 0 357 1287
Size: 357 930
NspI r_CATG'y
Cuts at: 0 561 1287
Size: 561 726
PasI CC'CwG_GG
Cuts at: 0 79 436 1113 1287
Size: 79 357 677 174
Fragments arranged by size:
677 357 174 79
PciI A'CATG_T
Cuts at: 0 557 1287
Size: 557 730
PflMI CCAn_nnn'nTGG
Cuts at: 0 128 1287
Size: 128 1159
PfoI T'CCnGG_A
Cuts at: 0 703 1287
Size: 703 584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 1287
Size: 1013 32 242
Fragments arranged by size:
1013 242 32
PpuMI rG'GwC_Cy
Cuts at: 0 963 1287
Size: 963 324
PsiI TTA'TAA
Cuts at: 0 551 1287
Size: 551 736
PvuII CAG'CTG
Cuts at: 0 6 827 1287
Size: 6 821 460
Fragments arranged by size:
821 460 6
SacI G_AGCT'C
Cuts at: 0 224 1287
Size: 224 1063
SapI GCTCTTCn'nnn_
Cuts at: 0 167 208 1287
Size: 167 41 1079
Fragments arranged by size:
1079 167 41
ScaI AGT'ACT
Cuts at: 0 664 1287
Size: 664 623
SfcI C'TryA_G
Cuts at: 0 1049 1287
Size: 1049 238
SmlI C'TyrA_G
Cuts at: 0 914 1287
Size: 914 373
StyI C'CwwG_G
Cuts at: 0 122 563 968 1287
Size: 122 441 405 319
Fragments arranged by size:
441 405 319 122
TatI w'GTAC_w
Cuts at: 0 596 662 1287
Size: 596 66 625
Fragments arranged by size:
625 596 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1145 1177 1287
Size: 1145 32 110
Fragments arranged by size:
1145 110 32
XmnI GAAnn'nnTTC
Cuts at: 0 202 1287
Size: 202 1085
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlfI AlwNI ApoI BanI
BanII BbvCI BcgI BciVI BglI BglII BlpI BmrI
BmtI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BssHII BstXI BstYI BtgI
BtgZI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KpnI MmeI MslI MspA1I NcoI NheI
NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI
PvuII SacI SapI ScaI SfcI SmlI StyI TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BclI BfrBI Bme1580I BmgBI
BplI BpmI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KasI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PmeI PmlI PshAI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI ZraI