Restriction map for
pFN21AE4415
(Linear) (Six-base) MAPSORT of: KIEE4415.seq Check: 3566 from: 1 to: 1287
KIEE4415.orf
With 182 enzymes: SgfI *
March 22, 2012 17:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 1220 1287
Size: 1134 86 67
Fragments arranged by size:
1134 86 67
AlwNI CAG_nnn'CTG
Cuts at: 0 301 345 657 1287
Size: 301 44 312 630
Fragments arranged by size:
630 312 301 44
ApaI G_GGCC'C
Cuts at: 0 457 1287
Size: 457 830
BamHI G'GATC_C
Cuts at: 0 989 1287
Size: 989 298
BanI G'GyrC_C
Cuts at: 0 1087 1287
Size: 1087 200
BanII G_rGCy'C
Cuts at: 0 292 457 628 1287
Size: 292 165 171 659
Fragments arranged by size:
659 292 171 165
BglI GCCn_nnn'nGGC
Cuts at: 0 824 1222 1287
Size: 824 398 65
Fragments arranged by size:
824 398 65
Bme1580I G_kGCm'C
Cuts at: 0 457 522 804 1071 1287
Size: 457 65 282 267 216
Fragments arranged by size:
457 282 267 216 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 744 1287
Size: 744 543
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1088 1287
Size: 1088 199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 464 1287
Size: 306 158 823
Fragments arranged by size:
823 306 158
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 652 1011 1287
Size: 652 359 276
Fragments arranged by size:
652 359 276
BseYI C'CCAG_C
Cuts at: 0 345 450 1138 1287
Size: 345 105 688 149
Fragments arranged by size:
688 345 149 105
BsiHKAI G_wGCw'C
Cuts at: 0 228 292 646 686 1183 1287
Size: 228 64 354 40 497 104
Fragments arranged by size:
497 354 228 104 64 40
Bsp1286I G_dGCh'C
Cuts at: 0 228 292 457 522 628 646 686 804
Size: 228 64 165 65 106 18 40 118
Cuts at: 804 1071 1183 1287
Size: 267 112 104
Fragments arranged by size:
267 228 165 118 112 106 104 65
64 40 18
BspHI T'CATG_A
Cuts at: 0 917 1287
Size: 917 370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 579 730 1287
Size: 579 151 557
Fragments arranged by size:
579 557 151
BsrDI GCAATG_nn'
Cuts at: 0 1034 1157 1287
Size: 1034 123 130
Fragments arranged by size:
1034 130 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 178 657 1287
Size: 178 479 630
Fragments arranged by size:
630 479 178
BstEII G'GTnAC_C
Cuts at: 0 694 1287
Size: 694 593
BstXI CCAn_nnnn'nTGG
Cuts at: 0 16 1287
Size: 16 1271
BstYI r'GATC_y
Cuts at: 0 122 989 1287
Size: 122 867 298
Fragments arranged by size:
867 298 122
BtgI C'CryG_G
Cuts at: 0 860 900 1287
Size: 860 40 387
Fragments arranged by size:
860 387 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 816 1287
Size: 816 471
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1065 1100 1287
Size: 1065 35 187
Fragments arranged by size:
1065 187 35
DraIII CAC_nnn'GTG
Cuts at: 0 410 526 1287
Size: 410 116 761
Fragments arranged by size:
761 410 116
EaeI y'GGCC_r
Cuts at: 0 1115 1214 1287
Size: 1115 99 73
Fragments arranged by size:
1115 99 73
EarI CTCTTCn'nnn_
Cuts at: 0 113 241 246 955 1287
Size: 113 128 5 709 332
Fragments arranged by size:
709 332 128 113 5
EcoICRI GAG'CTC
Cuts at: 0 290 1287
Size: 290 997
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 464 1134 1220 1287
Size: 306 158 670 86 67
Fragments arranged by size:
670 306 158 86 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 754 1287
Size: 337 417 533
Fragments arranged by size:
533 417 337
EcoO109I rG'GnC_Cy
Cuts at: 0 369 454 816 1287
Size: 369 85 362 471
Fragments arranged by size:
471 369 362 85
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 645 1287
Size: 645 642
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 210 242 1287
Size: 210 32 1045
Fragments arranged by size:
1045 210 32
HincII GTy'rAC
Cuts at: 0 270 425 1287
Size: 270 155 862
Fragments arranged by size:
862 270 155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 905 1156 1287
Size: 561 344 251 131
Fragments arranged by size:
561 344 251 131
MscI TGG'CCA
Cuts at: 0 1117 1287
Size: 1117 170
MslI CAynn'nnrTG
Cuts at: 0 934 1258 1287
Size: 934 324 29
Fragments arranged by size:
934 324 29
MspA1I CmG'CkG
Cuts at: 0 491 838 1287
Size: 491 347 449
Fragments arranged by size:
491 449 347
NcoI C'CATG_G
Cuts at: 0 860 900 1287
Size: 860 40 387
Fragments arranged by size:
860 387 40
PasI CC'CwG_GG
Cuts at: 0 9 339 1287
Size: 9 330 948
Fragments arranged by size:
948 330 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 986 1287
Size: 345 641 301
Fragments arranged by size:
641 345 301
PfoI T'CCnGG_A
Cuts at: 0 755 1287
Size: 755 532
PpuMI rG'GwC_Cy
Cuts at: 0 369 1287
Size: 369 918
PspOMI G'GGCC_C
Cuts at: 0 453 1287
Size: 453 834
PstI C_TGCA'G
Cuts at: 0 653 1287
Size: 653 634
PvuII CAG'CTG
Cuts at: 0 491 838 1287
Size: 491 347 449
Fragments arranged by size:
491 449 347
SacI G_AGCT'C
Cuts at: 0 292 1287
Size: 292 995
SapI GCTCTTCn'nnn_
Cuts at: 0 241 1287
Size: 241 1046
SbfI CC_TGCA'GG
Cuts at: 0 653 1287
Size: 653 634
SfcI C'TryA_G
Cuts at: 0 60 486 649 1170 1287
Size: 60 426 163 521 117
Fragments arranged by size:
521 426 163 117 60
StyI C'CwwG_G
Cuts at: 0 860 900 1287
Size: 860 40 387
Fragments arranged by size:
860 387 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 669 1182 1287
Size: 669 513 105
Fragments arranged by size:
669 513 105
TatI w'GTAC_w
Cuts at: 0 727 1287
Size: 727 560
Tth111I GACn'n_nGTC
Cuts at: 0 936 1287
Size: 936 351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 435 1287
Size: 435 852
Enzymes that do cut:
AcuI AlwNI ApaI BamHI BanI BanII BglI Bme1580I
BmrI BplI BpmI BseRI BseYI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI
CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI Hin4I HincII MmeI MscI MslI MspA1I NcoI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SapI SbfI SfcI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI ClaI
DraI DrdI EagI EciI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmaI XmnI ZraI