Restriction map for
pFN21AE4412
(Linear) (Six-base) MAPSORT of: KIEE4412.seq Check: 9988 from: 1 to: 1275
KIEE4412.orf
With 182 enzymes: SgfI *
March 22, 2012 17:56 ..
AccI GT'mk_AC
Cuts at: 0 329 1275
Size: 329 946
Acc65I G'GTAC_C
Cuts at: 0 586 1275
Size: 586 689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 1275
Size: 406 869
AlwNI CAG_nnn'CTG
Cuts at: 0 1221 1275
Size: 1221 54
ApoI r'AATT_y
Cuts at: 0 715 1275
Size: 715 560
AvaI C'yCGr_G
Cuts at: 0 1050 1275
Size: 1050 225
BamHI G'GATC_C
Cuts at: 0 1172 1275
Size: 1172 103
BanI G'GyrC_C
Cuts at: 0 130 444 586 1275
Size: 130 314 142 689
Fragments arranged by size:
689 314 142 130
BanII G_rGCy'C
Cuts at: 0 350 665 797 1080 1275
Size: 350 315 132 283 195
Fragments arranged by size:
350 315 283 195 132
BbeI G_GCGC'C
Cuts at: 0 134 1275
Size: 134 1141
BbsI GAAGACnn'nnnn_
Cuts at: 0 9 504 1275
Size: 9 495 771
Fragments arranged by size:
771 495 9
BbvCI CC'TCA_GC
Cuts at: 0 811 1275
Size: 811 464
BclI T'GATC_A
Cuts at: 0 47 1275
Size: 47 1228
BglI GCCn_nnn'nGGC
Cuts at: 0 677 1275
Size: 677 598
BlpI GC'TnA_GC
Cuts at: 0 148 1275
Size: 148 1127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 879 1275
Size: 879 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 445 724 1275
Size: 313 132 279 551
Fragments arranged by size:
551 313 279 132
Bpu10I CC'TnA_GC
Cuts at: 0 811 1275
Size: 811 464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1275
Size: 583 692
BsaHI Gr'CG_yC
Cuts at: 0 131 1275
Size: 131 1144
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 310 313 1275
Size: 136 174 3 962
Fragments arranged by size:
962 174 136 3
BseYI C'CCAG_C
Cuts at: 0 633 955 1275
Size: 633 322 320
Fragments arranged by size:
633 322 320
BsiEI CG_ry'CG
Cuts at: 0 756 1275
Size: 756 519
BsiHKAI G_wGCw'C
Cuts at: 0 95 350 656 665 797 1275
Size: 95 255 306 9 132 478
Fragments arranged by size:
478 306 255 132 95 9
BsmI GAATG_Cn'
Cuts at: 0 225 623 1275
Size: 225 398 652
Fragments arranged by size:
652 398 225
BsmBI CGTCTCn'nnnn_
Cuts at: 0 421 1170 1275
Size: 421 749 105
Fragments arranged by size:
749 421 105
Bsp1286I G_dGCh'C
Cuts at: 0 95 350 656 665 797 1080 1275
Size: 95 255 306 9 132 283 195
Fragments arranged by size:
306 283 255 195 132 95 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 548 1275
Size: 548 727
BsrFI r'CCGG_y
Cuts at: 0 1121 1275
Size: 1121 154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 1275
Size: 99 1176
BstEII G'GTnAC_C
Cuts at: 0 1210 1275
Size: 1210 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 836 933 1275
Size: 836 97 342
Fragments arranged by size:
836 342 97
BstYI r'GATC_y
Cuts at: 0 386 1148 1172 1275
Size: 386 762 24 103
Fragments arranged by size:
762 386 103 24
BtgI C'CryG_G
Cuts at: 0 153 1275
Size: 153 1122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 83 974 1275
Size: 83 891 301
Fragments arranged by size:
891 301 83
DrdI GACnn_nn'nnGTC
Cuts at: 0 319 435 1275
Size: 319 116 840
Fragments arranged by size:
840 319 116
EaeI y'GGCC_r
Cuts at: 0 74 156 203 923 1275
Size: 74 82 47 720 352
Fragments arranged by size:
720 352 82 74 47
EarI CTCTTCn'nnn_
Cuts at: 0 661 1103 1275
Size: 661 442 172
Fragments arranged by size:
661 442 172
EcoICRI GAG'CTC
Cuts at: 0 348 663 795 1275
Size: 348 315 132 480
Fragments arranged by size:
480 348 315 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 445 724 1275
Size: 313 132 279 551
Fragments arranged by size:
551 313 279 132
EcoO109I rG'GnC_Cy
Cuts at: 0 1218 1275
Size: 1218 57
EcoRV GAT'ATC
Cuts at: 0 941 1275
Size: 941 334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1083 1275
Size: 1083 192
FseI GG_CCGG'CC
Cuts at: 0 1125 1275
Size: 1125 150
HaeII r_GCGC'y
Cuts at: 0 134 480 1001 1275
Size: 134 346 521 274
Fragments arranged by size:
521 346 274 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 784 816 952 984 1275
Size: 784 32 136 32 291
Fragments arranged by size:
784 291 136 32 32
HincII GTy'rAC
Cuts at: 0 537 1275
Size: 537 738
KasI G'GCGC_C
Cuts at: 0 130 1275
Size: 130 1145
KpnI G_GTAC'C
Cuts at: 0 590 1275
Size: 590 685
MscI TGG'CCA
Cuts at: 0 76 205 925 1275
Size: 76 129 720 350
Fragments arranged by size:
720 350 129 76
MspA1I CmG'CkG
Cuts at: 0 117 144 1275
Size: 117 27 1131
Fragments arranged by size:
1131 117 27
NaeI GCC'GGC
Cuts at: 0 1123 1275
Size: 1123 152
NarI GG'CG_CC
Cuts at: 0 131 1275
Size: 131 1144
NgoMIV G'CCGG_C
Cuts at: 0 1121 1275
Size: 1121 154
PasI CC'CwG_GG
Cuts at: 0 1226 1275
Size: 1226 49
PfoI T'CCnGG_A
Cuts at: 0 308 1275
Size: 308 967
PstI C_TGCA'G
Cuts at: 0 395 1275
Size: 395 880
SacI G_AGCT'C
Cuts at: 0 350 665 797 1275
Size: 350 315 132 478
Fragments arranged by size:
478 350 315 132
SapI GCTCTTCn'nnn_
Cuts at: 0 661 1275
Size: 661 614
SbfI CC_TGCA'GG
Cuts at: 0 395 1275
Size: 395 880
ScaI AGT'ACT
Cuts at: 0 1066 1275
Size: 1066 209
SfcI C'TryA_G
Cuts at: 0 391 1275
Size: 391 884
SfoI GGC'GCC
Cuts at: 0 132 1275
Size: 132 1143
SmaI CCC'GGG
Cuts at: 0 1052 1275
Size: 1052 223
SmlI C'TyrA_G
Cuts at: 0 598 1275
Size: 598 677
StyI C'CwwG_G
Cuts at: 0 896 1275
Size: 896 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 465 832 1258 1275
Size: 465 367 426 17
Fragments arranged by size:
465 426 367 17
TatI w'GTAC_w
Cuts at: 0 239 398 1064 1275
Size: 239 159 666 211
Fragments arranged by size:
666 239 211 159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 1275
Size: 231 1044
XmaI C'CCGG_G
Cuts at: 0 1050 1275
Size: 1050 225
Enzymes that do cut:
AccI Acc65I AlfI AlwNI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BclI BglI BlpI BplI
BpmI Bpu10I BpuEI BsaHI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrFI BstAPI BstEII BstXI
BstYI BtgI BtgZI DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRV FalI FseI HaeII Hin4I HincII KasI
KpnI MscI MspA1I NaeI NarI NgoMIV PasI PfoI
PstI SacI SapI SbfI ScaI SfcI SfoI SmaI
SmlI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BfrBI BglII Bme1580I BmgBI
BmrI BmtI BsaI BsaAI BsaBI BsaWI BsaXI BsgI
BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
EagI EciI EcoNI EcoRI FspI FspAI HindIII HpaI
MfeI MluI MmeI MslI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI