Restriction map for
pFN21AE4411
(Linear) (Six-base) MAPSORT of: KIEE4411.seq Check: 484 from: 1 to: 1272
KIEE4411.orf
With 182 enzymes: SgfI *
March 22, 2012 17:56 ..
AccI GT'mk_AC
Cuts at: 0 563 1040 1272
Size: 563 477 232
Fragments arranged by size:
563 477 232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 638 1272
Size: 337 301 634
Fragments arranged by size:
634 337 301
AflIII A'CryG_T
Cuts at: 0 841 1272
Size: 841 431
ApoI r'AATT_y
Cuts at: 0 848 877 1272
Size: 848 29 395
Fragments arranged by size:
848 395 29
AvaI C'yCGr_G
Cuts at: 0 827 1272
Size: 827 445
AvrII C'CTAG_G
Cuts at: 0 573 1272
Size: 573 699
BamHI G'GATC_C
Cuts at: 0 1200 1272
Size: 1200 72
BanI G'GyrC_C
Cuts at: 0 774 1272
Size: 774 498
BanII G_rGCy'C
Cuts at: 0 51 1272
Size: 51 1221
BbsI GAAGACnn'nnnn_
Cuts at: 0 41 92 1272
Size: 41 51 1180
Fragments arranged by size:
1180 51 41
BglI GCCn_nnn'nGGC
Cuts at: 0 578 783 1272
Size: 578 205 489
Fragments arranged by size:
578 489 205
BlpI GC'TnA_GC
Cuts at: 0 683 1272
Size: 683 589
Bme1580I G_kGCm'C
Cuts at: 0 793 1272
Size: 793 479
BmrI ACTGGGnnnn_n'
Cuts at: 0 1000 1272
Size: 1000 272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 1272
Size: 624 648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 737 787 1017 1272
Size: 137 600 50 230 255
Fragments arranged by size:
600 255 230 137 50
BsaAI yAC'GTr
Cuts at: 0 1230 1272
Size: 1230 42
BsaWI w'CCGG_w
Cuts at: 0 506 1272
Size: 506 766
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 785 815 1272
Size: 785 30 457
Fragments arranged by size:
785 457 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 1168 1272
Size: 511 657 104
Fragments arranged by size:
657 511 104
BseYI C'CCAG_C
Cuts at: 0 56 60 916 1272
Size: 56 4 856 356
Fragments arranged by size:
856 356 56 4
BsiHKAI G_wGCw'C
Cuts at: 0 723 1272
Size: 723 549
Bsp1286I G_dGCh'C
Cuts at: 0 51 723 793 1272
Size: 51 672 70 479
Fragments arranged by size:
672 479 70 51
BspEI T'CCGG_A
Cuts at: 0 506 1272
Size: 506 766
BsrBI CCG'CTC
Cuts at: 0 113 1272
Size: 113 1159
BsrGI T'GTAC_A
Cuts at: 0 543 1272
Size: 543 729
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1120 1272
Size: 1120 152
BstYI r'GATC_y
Cuts at: 0 1200 1272
Size: 1200 72
BtgI C'CryG_G
Cuts at: 0 9 1143 1272
Size: 9 1134 129
Fragments arranged by size:
1134 129 9
BtsI GCAGTG_nn'
Cuts at: 0 821 1053 1256 1272
Size: 821 232 203 16
Fragments arranged by size:
821 232 203 16
ClaI AT'CG_AT
Cuts at: 0 752 1272
Size: 752 520
DraI TTT'AAA
Cuts at: 0 1131 1272
Size: 1131 141
EaeI y'GGCC_r
Cuts at: 0 407 1272
Size: 407 865
EarI CTCTTCn'nnn_
Cuts at: 0 305 1272
Size: 305 967
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 337 638 737 787 1017 1272
Size: 137 200 301 99 50 230 255
Fragments arranged by size:
301 255 230 200 137 99 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 572 964 1272
Size: 572 392 308
Fragments arranged by size:
572 392 308
EcoO109I rG'GnC_Cy
Cuts at: 0 704 978 1272
Size: 704 274 294
Fragments arranged by size:
704 294 274
EcoRI G'AATT_C
Cuts at: 0 848 1272
Size: 848 424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 627 970 1272
Size: 627 343 302
Fragments arranged by size:
627 343 302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 990 1022 1272
Size: 226 32 732 32 250
Fragments arranged by size:
732 250 226 32 32
HincII GTy'rAC
Cuts at: 0 1041 1272
Size: 1041 231
MfeI C'AATT_G
Cuts at: 0 466 1272
Size: 466 806
MscI TGG'CCA
Cuts at: 0 409 1272
Size: 409 863
MslI CAynn'nnrTG
Cuts at: 0 14 1272
Size: 14 1258
MspA1I CmG'CkG
Cuts at: 0 60 1272
Size: 60 1212
NcoI C'CATG_G
Cuts at: 0 1143 1272
Size: 1143 129
NspI r_CATG'y
Cuts at: 0 175 845 1272
Size: 175 670 427
Fragments arranged by size:
670 427 175
PciI A'CATG_T
Cuts at: 0 841 1272
Size: 841 431
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 1272
Size: 771 501
PfoI T'CCnGG_A
Cuts at: 0 1112 1272
Size: 1112 160
PmlI CAC'GTG
Cuts at: 0 1230 1272
Size: 1230 42
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 944 976 1272
Size: 944 32 296
Fragments arranged by size:
944 296 32
PpuMI rG'GwC_Cy
Cuts at: 0 704 1272
Size: 704 568
PstI C_TGCA'G
Cuts at: 0 1225 1272
Size: 1225 47
PvuII CAG'CTG
Cuts at: 0 60 1272
Size: 60 1212
SalI G'TCGA_C
Cuts at: 0 1039 1272
Size: 1039 233
SapI GCTCTTCn'nnn_
Cuts at: 0 305 1272
Size: 305 967
SfcI C'TryA_G
Cuts at: 0 1215 1221 1272
Size: 1215 6 51
Fragments arranged by size:
1215 51 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 578 1272
Size: 578 694
SmaI CCC'GGG
Cuts at: 0 829 1272
Size: 829 443
StyI C'CwwG_G
Cuts at: 0 573 1143 1272
Size: 573 570 129
Fragments arranged by size:
573 570 129
TatI w'GTAC_w
Cuts at: 0 543 1272
Size: 543 729
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 785 817 1272
Size: 785 32 455
Fragments arranged by size:
785 455 32
XmaI C'CCGG_G
Cuts at: 0 827 1272
Size: 827 445
XmnI GAAnn'nnTTC
Cuts at: 0 89 740 1272
Size: 89 651 532
Fragments arranged by size:
651 532 89
Enzymes that do cut:
AccI AcuI AflIII ApoI AvaI AvrII BamHI BanI
BanII BbsI BglI BlpI Bme1580I BmrI BplI BpmI
BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI
BsrBI BsrGI BstXI BstYI BtgI BtsI ClaI DraI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII MfeI MscI MslI MspA1I NcoI NspI PciI
PflMI PfoI PmlI PpiI PpuMI PstI PvuII SalI
SapI SfcI SfiI SmaI StyI TatI TstI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI Bpu10I BpuEI BsaI BsaBI BsaHI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI CspCI DraIII DrdI EagI EciI EcoICRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SanDI SbfI
ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XhoI ZraI