(Linear) (Six-base) MAPSORT of: KIEE4410.seq  Check: 4048  from: 1  to: 1269



KIEE4410.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     917    1269 
   Size:        917     352
 
AfeI AGC'GCT

Cuts at:      0     159    1138    1269 
   Size:        159     979     131

  Fragments arranged by size: 

                 979     159     131
 
ApaI G_GGCC'C

Cuts at:      0    1151    1269 
   Size:       1151     118
 
ApoI r'AATT_y

Cuts at:      0     373     824    1269 
   Size:        373     451     445

  Fragments arranged by size: 

                 451     445     373
 
AvaI C'yCGr_G

Cuts at:      0     273    1269 
   Size:        273     996
 
BanII G_rGCy'C

Cuts at:      0      35    1151    1269 
   Size:         35    1116     118

  Fragments arranged by size: 

                1116     118      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1062    1269 
   Size:       1062     207
 
BbvCI CC'TCA_GC

Cuts at:      0     323    1269 
   Size:        323     946
 
BglI GCCn_nnn'nGGC

Cuts at:      0     745    1269 
   Size:        745     524
 
BlpI GC'TnA_GC

Cuts at:      0     439    1269 
   Size:        439     830
 
Bme1580I G_kGCm'C

Cuts at:      0    1151    1269 
   Size:       1151     118
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1212    1269 
   Size:       1212      57
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1155    1269 
   Size:       1155     114
 
Bpu10I CC'TnA_GC

Cuts at:      0     323    1269 
   Size:        323     946
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     815    1269 
   Size:        815     454
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     712    1269 
   Size:        712     557
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1147    1269 
   Size:       1147     122
 
BsiHKAI G_wGCw'C

Cuts at:      0     818    1269 
   Size:        818     451
 
BsmI GAATG_Cn'

Cuts at:      0     838    1269 
   Size:        838     431
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     930    1269 
   Size:        930     339
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     818    1151    1269 
   Size:         35     783     333     118

  Fragments arranged by size: 

                 783     333     118      35
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     882    1269 
   Size:        882     387
 
BsrDI GCAATG_nn'

Cuts at:      0     572    1269 
   Size:        572     697
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1243    1269 
   Size:       1243      26
 
BstYI r'GATC_y

Cuts at:      0     200    1269 
   Size:        200    1069
 
DraI TTT'AAA

Cuts at:      0     641    1269 
   Size:        641     628
 
EarI CTCTTCn'nnn_

Cuts at:      0     592     921    1269 
   Size:        592     329     348

  Fragments arranged by size: 

                 592     348     329
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      91    1269 
   Size:         91    1178
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     917    1269 
   Size:        917     352
 
EcoO109I rG'GnC_Cy

Cuts at:      0     747    1147    1148    1170    1269 
   Size:        747     400       1      22      99

  Fragments arranged by size: 

                 747     400      99      22       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     912    1269 
   Size:        912     357
 
HaeII r_GCGC'y

Cuts at:      0     161    1140    1269 
   Size:        161     979     129

  Fragments arranged by size: 

                 979     161     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     976    1008    1269 
   Size:        256      32     688      32     261

  Fragments arranged by size: 

                 688     261     256      32      32
 
HincII GTy'rAC

Cuts at:      0     501    1269 
   Size:        501     768
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     252    1269 
   Size:        171      81    1017

  Fragments arranged by size: 

                1017     171      81
 
MspA1I CmG'CkG

Cuts at:      0      18     955    1269 
   Size:         18     937     314

  Fragments arranged by size: 

                 937     314      18
 
PsiI TTA'TAA

Cuts at:      0     773    1269 
   Size:        773     496
 
PspOMI G'GGCC_C

Cuts at:      0    1147    1269 
   Size:       1147     122
 
PstI C_TGCA'G

Cuts at:      0     861    1269 
   Size:        861     408
 
PvuII CAG'CTG

Cuts at:      0      18    1269 
   Size:         18    1251
 
SfcI C'TryA_G

Cuts at:      0     857    1269 
   Size:        857     412
 
SmlI C'TyrA_G

Cuts at:      0     830    1269 
   Size:        830     439
 
StyI C'CwwG_G

Cuts at:      0    1236    1269 
   Size:       1236      33
 
XmnI GAAnn'nnTTC

Cuts at:      0     680    1269 
   Size:        680     589
 

 Enzymes that do cut:

     AcuI     AfeI     ApaI     ApoI     AvaI    BanII     BbsI    BbvCI
     BglI     BlpI Bme1580I     BmrI     BplI   Bpu10I    BpuEI     BsaI
    BseRI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BstXI
    BstYI     DraI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I   HincII     MmeI   MspA1I     PsiI   PspOMI     PstI    PvuII
     SfcI     SmlI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI    BglII    BmgBI     BmtI     BpmI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI