Restriction map for
pFN21AE4407
(Linear) (Six-base) MAPSORT of: KIEE4407.seq Check: 448 from: 1 to: 1263
KIEE4407.orf
With 182 enzymes: SgfI *
March 22, 2012 17:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 541 550 1263
Size: 94 447 9 713
Fragments arranged by size:
713 447 94 9
AhdI GACnn_n'nnGTC
Cuts at: 0 676 948 1263
Size: 676 272 315
Fragments arranged by size:
676 315 272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1225 1263
Size: 1225 38
AlwNI CAG_nnn'CTG
Cuts at: 0 918 1263
Size: 918 345
ApaI G_GGCC'C
Cuts at: 0 894 1263
Size: 894 369
ApoI r'AATT_y
Cuts at: 0 350 1263
Size: 350 913
AvaI C'yCGr_G
Cuts at: 0 275 787 893 1263
Size: 275 512 106 370
Fragments arranged by size:
512 370 275 106
BanI G'GyrC_C
Cuts at: 0 229 1237 1263
Size: 229 1008 26
Fragments arranged by size:
1008 229 26
BanII G_rGCy'C
Cuts at: 0 449 595 695 894 1263
Size: 449 146 100 199 369
Fragments arranged by size:
449 369 199 146 100
BbeI G_GCGC'C
Cuts at: 0 1241 1263
Size: 1241 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 615 1263
Size: 615 648
BglII A'GATC_T
Cuts at: 0 44 1263
Size: 44 1219
BlpI GC'TnA_GC
Cuts at: 0 1099 1263
Size: 1099 164
Bme1580I G_kGCm'C
Cuts at: 0 477 894 1214 1263
Size: 477 417 320 49
Fragments arranged by size:
477 417 320 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 896 1263
Size: 896 367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 562 1263
Size: 412 150 701
Fragments arranged by size:
701 412 150
Bpu10I CC'TnA_GC
Cuts at: 0 89 1263
Size: 89 1174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 1263
Size: 466 797
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 726 1263
Size: 592 134 537
Fragments arranged by size:
592 537 134
BsaHI Gr'CG_yC
Cuts at: 0 1238 1263
Size: 1238 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 293 307 811 1099 1263
Size: 293 14 504 288 164
Fragments arranged by size:
504 293 288 164 14
BseYI C'CCAG_C
Cuts at: 0 476 636 1263
Size: 476 160 627
Fragments arranged by size:
627 476 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1045 1236 1263
Size: 1025 20 191 27
Fragments arranged by size:
1025 191 27 20
BsiHKAI G_wGCw'C
Cuts at: 0 595 1263
Size: 595 668
Bsp1286I G_dGCh'C
Cuts at: 0 449 477 595 695 894 1214 1263
Size: 449 28 118 100 199 320 49
Fragments arranged by size:
449 320 199 118 100 49 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 974 1263
Size: 974 289
BsrBI CCG'CTC
Cuts at: 0 561 1263
Size: 561 702
BsrDI GCAATG_nn'
Cuts at: 0 750 1134 1263
Size: 750 384 129
Fragments arranged by size:
750 384 129
BsrFI r'CCGG_y
Cuts at: 0 613 1263
Size: 613 650
BstAPI GCAn_nnn'nTGC
Cuts at: 0 918 1263
Size: 918 345
BstEII G'GTnAC_C
Cuts at: 0 714 1263
Size: 714 549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1161 1263
Size: 1161 102
BstYI r'GATC_y
Cuts at: 0 44 1263
Size: 44 1219
Bsu36I CC'TnA_GG
Cuts at: 0 69 1263
Size: 69 1194
BtgI C'CryG_G
Cuts at: 0 970 1263
Size: 970 293
EaeI y'GGCC_r
Cuts at: 0 128 410 615 928 1151 1263
Size: 128 282 205 313 223 112
Fragments arranged by size:
313 282 223 205 128 112
EarI CTCTTCn'nnn_
Cuts at: 0 160 583 626 1263
Size: 160 423 43 637
Fragments arranged by size:
637 423 160 43
EcoICRI GAG'CTC
Cuts at: 0 593 1263
Size: 593 670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 412 541 550 562 1263
Size: 94 318 129 9 12 701
Fragments arranged by size:
701 318 129 94 12 9
EcoO109I rG'GnC_Cy
Cuts at: 0 315 890 1263
Size: 315 575 373
Fragments arranged by size:
575 373 315
EcoRI G'AATT_C
Cuts at: 0 350 1263
Size: 350 913
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 66 171 726 1263
Size: 66 105 555 537
Fragments arranged by size:
555 537 105 66
HaeII r_GCGC'y
Cuts at: 0 275 1137 1241 1263
Size: 275 862 104 22
Fragments arranged by size:
862 275 104 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 662 694 934 966 1263
Size: 662 32 240 32 297
Fragments arranged by size:
662 297 240 32 32
KasI G'GCGC_C
Cuts at: 0 1237 1263
Size: 1237 26
MscI TGG'CCA
Cuts at: 0 130 1153 1263
Size: 130 1023 110
Fragments arranged by size:
1023 130 110
MslI CAynn'nnrTG
Cuts at: 0 235 1263
Size: 235 1028
MspA1I CmG'CkG
Cuts at: 0 504 972 1009 1263
Size: 504 468 37 254
Fragments arranged by size:
504 468 254 37
NaeI GCC'GGC
Cuts at: 0 615 1263
Size: 615 648
NarI GG'CG_CC
Cuts at: 0 1238 1263
Size: 1238 25
NgoMIV G'CCGG_C
Cuts at: 0 613 1263
Size: 613 650
PasI CC'CwG_GG
Cuts at: 0 682 695 1263
Size: 682 13 568
Fragments arranged by size:
682 568 13
PfoI T'CCnGG_A
Cuts at: 0 626 1263
Size: 626 637
PpuMI rG'GwC_Cy
Cuts at: 0 315 1263
Size: 315 948
PspOMI G'GGCC_C
Cuts at: 0 890 1263
Size: 890 373
PspXI vC'TCGA_Gb
Cuts at: 0 275 1263
Size: 275 988
PstI C_TGCA'G
Cuts at: 0 98 923 1263
Size: 98 825 340
Fragments arranged by size:
825 340 98
PvuII CAG'CTG
Cuts at: 0 504 1009 1263
Size: 504 505 254
Fragments arranged by size:
505 504 254
SacI G_AGCT'C
Cuts at: 0 595 1263
Size: 595 668
SacII CC_GC'GG
Cuts at: 0 973 1263
Size: 973 290
SapI GCTCTTCn'nnn_
Cuts at: 0 160 583 626 1263
Size: 160 423 43 637
Fragments arranged by size:
637 423 160 43
SexAI A'CCwGG_T
Cuts at: 0 428 1263
Size: 428 835
SfcI C'TryA_G
Cuts at: 0 94 919 1263
Size: 94 825 344
Fragments arranged by size:
825 344 94
SfoI GGC'GCC
Cuts at: 0 1239 1263
Size: 1239 24
SmaI CCC'GGG
Cuts at: 0 789 895 1263
Size: 789 106 368
Fragments arranged by size:
789 368 106
SmlI C'TyrA_G
Cuts at: 0 275 481 1263
Size: 275 206 782
Fragments arranged by size:
782 275 206
StuI AGG'CCT
Cuts at: 0 652 841 1263
Size: 652 189 422
Fragments arranged by size:
652 422 189
StyI C'CwwG_G
Cuts at: 0 77 836 1121 1263
Size: 77 759 285 142
Fragments arranged by size:
759 285 142 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 891 969 1263
Size: 891 78 294
Fragments arranged by size:
891 294 78
TatI w'GTAC_w
Cuts at: 0 296 1263
Size: 296 967
Tth111I GACn'n_nGTC
Cuts at: 0 712 1263
Size: 712 551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 702 1263
Size: 702 561
XhoI C'TCGA_G
Cuts at: 0 275 1263
Size: 275 988
XmaI C'CCGG_G
Cuts at: 0 787 893 1263
Size: 787 106 370
Fragments arranged by size:
787 370 106
XmnI GAAnn'nnTTC
Cuts at: 0 550 1263
Size: 550 713
Enzymes that do cut:
AcuI AhdI AlfI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI Bsu36I BtgI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NgoMIV PasI PfoI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SapI SexAI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BmgBI BmtI BplI BsaAI BsaBI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
ZraI