Restriction map for
pFN21AE4402
(Linear) (Six-base) MAPSORT of: KIEE4402.seq Check: 5562 from: 1 to: 1251
KIEE4402.orf
With 182 enzymes: SgfI *
March 22, 2012 17:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1251
Size: 613 638
AfeI AGC'GCT
Cuts at: 0 150 1251
Size: 150 1101
AflIII A'CryG_T
Cuts at: 0 564 1251
Size: 564 687
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 240 1251
Size: 240 1011
AlwNI CAG_nnn'CTG
Cuts at: 0 643 1251
Size: 643 608
ApaLI G'TGCA_C
Cuts at: 0 982 1251
Size: 982 269
ApoI r'AATT_y
Cuts at: 0 746 1251
Size: 746 505
AvaI C'yCGr_G
Cuts at: 0 36 144 539 722 1251
Size: 36 108 395 183 529
Fragments arranged by size:
529 395 183 108 36
BciVI GTATCCnnnnn_n'
Cuts at: 0 149 277 925 1251
Size: 149 128 648 326
Fragments arranged by size:
648 326 149 128
BlpI GC'TnA_GC
Cuts at: 0 1003 1251
Size: 1003 248
Bme1580I G_kGCm'C
Cuts at: 0 964 986 1251
Size: 964 22 265
Fragments arranged by size:
964 265 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 512 1054 1085 1219 1251
Size: 347 165 542 31 134 32
Fragments arranged by size:
542 347 165 134 32 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 77 331 1251
Size: 77 254 920
Fragments arranged by size:
920 254 77
BsaHI Gr'CG_yC
Cuts at: 0 1194 1251
Size: 1194 57
BsaWI w'CCGG_w
Cuts at: 0 99 1251
Size: 99 1152
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 32 62 898 928 1251
Size: 32 30 836 30 323
Fragments arranged by size:
836 323 32 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 805 1082 1251
Size: 349 456 277 169
Fragments arranged by size:
456 349 277 169
BseYI C'CCAG_C
Cuts at: 0 82 204 902 1251
Size: 82 122 698 349
Fragments arranged by size:
698 349 122 82
BsiEI CG_ry'CG
Cuts at: 0 100 1251
Size: 100 1151
BsiHKAI G_wGCw'C
Cuts at: 0 986 1251
Size: 986 265
Bsp1286I G_dGCh'C
Cuts at: 0 964 986 1251
Size: 964 22 265
Fragments arranged by size:
964 265 22
BsrBI CCG'CTC
Cuts at: 0 67 1251
Size: 67 1184
BssSI C'ACGA_G
Cuts at: 0 740 1251
Size: 740 511
BstAPI GCAn_nnn'nTGC
Cuts at: 0 214 1251
Size: 214 1037
BstEII G'GTnAC_C
Cuts at: 0 598 1251
Size: 598 653
BstXI CCAn_nnnn'nTGG
Cuts at: 0 898 1057 1251
Size: 898 159 194
Fragments arranged by size:
898 194 159
BstYI r'GATC_y
Cuts at: 0 426 1028 1251
Size: 426 602 223
Fragments arranged by size:
602 426 223
BtgI C'CryG_G
Cuts at: 0 422 815 1251
Size: 422 393 436
Fragments arranged by size:
436 422 393
BtsI GCAGTG_nn'
Cuts at: 0 1076 1251
Size: 1076 175
ClaI AT'CG_AT
Cuts at: 0 1160 1251
Size: 1160 91
DraIII CAC_nnn'GTG
Cuts at: 0 586 1251
Size: 586 665
EaeI y'GGCC_r
Cuts at: 0 59 188 291 498 730 1251
Size: 59 129 103 207 232 521
Fragments arranged by size:
521 232 207 129 103 59
EarI CTCTTCn'nnn_
Cuts at: 0 760 832 1251
Size: 760 72 419
Fragments arranged by size:
760 419 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 18 1251
Size: 18 1233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 512 613 1054 1085 1219 1251
Size: 347 165 101 441 31 134 32
Fragments arranged by size:
441 347 165 134 101 32 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 871 1251
Size: 871 380
EcoRV GAT'ATC
Cuts at: 0 1215 1251
Size: 1215 36
HaeII r_GCGC'y
Cuts at: 0 152 1251
Size: 152 1099
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 123 155 243 275 971 1003
Size: 32 32 59 32 88 32 696 32
Cuts at: 1003 1251
Size: 248
Fragments arranged by size:
696 248 88 59 32 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 229 1251
Size: 120 109 1022
Fragments arranged by size:
1022 120 109
MscI TGG'CCA
Cuts at: 0 190 293 732 1251
Size: 190 103 439 519
Fragments arranged by size:
519 439 190 103
MslI CAynn'nnrTG
Cuts at: 0 758 1251
Size: 758 493
MspA1I CmG'CkG
Cuts at: 0 82 1251
Size: 82 1169
NcoI C'CATG_G
Cuts at: 0 815 1251
Size: 815 436
PflMI CCAn_nnn'nTGG
Cuts at: 0 248 1251
Size: 248 1003
PfoI T'CCnGG_A
Cuts at: 0 1031 1251
Size: 1031 220
PspXI vC'TCGA_Gb
Cuts at: 0 539 1251
Size: 539 712
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 432 464 1251
Size: 432 32 787
Fragments arranged by size:
787 432 32
PstI C_TGCA'G
Cuts at: 0 711 1251
Size: 711 540
SapI GCTCTTCn'nnn_
Cuts at: 0 760 1251
Size: 760 491
SfcI C'TryA_G
Cuts at: 0 618 707 1251
Size: 618 89 544
Fragments arranged by size:
618 544 89
SmaI CCC'GGG
Cuts at: 0 38 1251
Size: 38 1213
SmlI C'TyrA_G
Cuts at: 0 539 722 1251
Size: 539 183 529
Fragments arranged by size:
539 529 183
StuI AGG'CCT
Cuts at: 0 866 1251
Size: 866 385
StyI C'CwwG_G
Cuts at: 0 815 1251
Size: 815 436
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 255 771 1133 1251
Size: 255 516 362 118
Fragments arranged by size:
516 362 255 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 898 930 1220 1251
Size: 898 32 290 31
Fragments arranged by size:
898 290 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 518 1251
Size: 518 733
XbaI T'CTAG_A
Cuts at: 0 429 484 1251
Size: 429 55 767
Fragments arranged by size:
767 429 55
XhoI C'TCGA_G
Cuts at: 0 539 722 1251
Size: 539 183 529
Fragments arranged by size:
539 529 183
XmaI C'CCGG_G
Cuts at: 0 36 1251
Size: 36 1215
XmnI GAAnn'nnTTC
Cuts at: 0 769 1251
Size: 769 482
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI ApaLI ApoI AvaI
BciVI BlpI Bme1580I BpmI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BssSI BstAPI
BstEII BstXI BstYI BtgI BtsI ClaI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoRV HaeII Hin4I MmeI
MscI MslI MspA1I NcoI PflMI PfoI PspXI PsrI
PstI SapI SfcI SmaI SmlI StuI StyI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BglII BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsgI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI BstZ17I
Bsu36I BtgZI CspCI DraI DrdI EagI EcoICRI EcoO109I
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI XcmI ZraI